FastQCFastQC Report
Sat 31 Dec 2022
SRR5846423.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846423.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142709
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT11290.7911203918463446No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC9610.6733983140516716No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2350.16467076358183436No Hit
TACAATGGGTGTAAGGCCATTAAATAAAAGCAGGATGACTTAGATATTTTTC2260.15836422369997688No Hit
ACGTTAGGGCTGGGTAGCTGGGATTACCGGGGCCTGCCACCACACTTTGCTA2220.155561317085818No Hit
TAAATGGGGTGTTCTTGTCTTTTACATTACAAAGGTTGGTTTAAAGCCAGGC2170.1520576838181194No Hit
GTAGGTGGGAGAGGTGGGGAAGAAAATAATTTAGAAAGCCTACATCGACTTT2110.14785332389688105No Hit
AACTCCGGGTGGACTGTGGATACTGAATCAGTGTACTATTGGCTGCAGAATT2070.14505041728272217No Hit
TTGAGGGGATAAAAGAGTGGCTTTAAAATTTGAAATACTGGGAAAAATATTT2050.14364896397564275No Hit
GTTGCAGGGGGCTAGACTTAGATTCATTAACTCAAATTCAATGCTTCTATCA2050.14364896397564275No Hit
CCATTAGGGAGCCAACGTCACTCACTAGGGAAAGGCAATTGGTAAATCCACT2040.14294823732210302No Hit
CGAGTAGCGGGGAAGCTCTTGGGATCCTAGAAGTATTCTTATTTTCTTCTCT2020.14154678401502357No Hit
GTAGTAGGGTATAACTCTTACTAACAATGTATAACAATTTGGGAAGAAGTTA2000.14014533070794413No Hit
ATGAGCGGGTAAGGGAGAAGAAGAGGTGAGAGTTTGTCAGTTGGGTTTTGTT2000.14014533070794413No Hit
AAGGTAGGGATTTAATGAGGAGTGTATCTTACCAAACAAAACAATGGAAATG2000.14014533070794413No Hit
GTTTGAGGGATACGAAAATTAGCTTTTTGTCCTTTTTATTTAAAAATGTTTT1960.13734242409378525No Hit
CGATGGGTAAAAATATAGGTTTTCCAGCACTGGGACTATGAAATTTGAGATT1910.13383879082608666No Hit
GTTAAGGGGTAAAAAGAATGAAAAAGTGCTTTTTCCTCAACTTGATAACTGT1880.13173661086546748No Hit
ATTCAAGGGTGCTACCAAATGGCAAAAAGCAACAATAAACAGTTTGATTTTT1880.13173661086546748No Hit
GCATGGGCTGCCAGTAGCGTAACAGTCATATTTGCTTTTATTCACATTCACC1860.13033515755838807No Hit
CAATACGGGCTTGTAATGTCTTTGTTAGAAGGAGTTTATATTAGGATTATGC1860.13033515755838807No Hit
ACTAGAGGGATTTGAAGTCCCTGGCACCTACAGCTTCAGAAAACATTAAACA1850.12963443090484833No Hit
TATTTTGGGCACTCAGTCACCATGGCTCCGTCTGTCAAACTCAACTCTTTTT1850.12963443090484833No Hit
GTTATAGGGGCGTAGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAA1830.1282329775977689No Hit
AAGTTTGGGATTGAGATATGATTCAAATTTCATAAAATCTCATAAAATCCTA1830.1282329775977689No Hit
GCGACAGGGATGTAAGGTAGACATTTAAAATACTTCCATATTTTATGGTTGC1810.12683152429068945No Hit
ATTGTGGGGGGAGCTCGTGTCCCTTTGCTGAATTAGTCATGTGCTGCATAGT1790.12543007098361No Hit
CTTACAGGGATTCAACAAAACCTTCAGGAAGCCCTTTGGAAGAAAAACACGA1770.12402861767653056No Hit
TTTGATGGGCAACAGCCTTTACTGCCATTTTTTTTTAAATGTTTTCACTCAG1770.12402861767653056No Hit
AGCTCGGGCTACTTGCTGATTGTACCTATTAAATATTTTATATTCAGTCAAG1760.12332789102299084No Hit
ACTGTTGGGATCTAATGGAATACTGTGTGGTCATTTAAATTTATATCATGGA1750.12262716436945112No Hit
ATTAATGGGGACAACAAAAAAATTTCTGTAATTTGACCTTATAGTGCTATAT1750.12262716436945112No Hit
GCAAGAGGGCAGATAAAGTTACTAGAGTACTGTATATACATTTTCCTTTCTA1750.12262716436945112No Hit
ACGAGGGACTTGGTGGCCATGTACTTGGAAAAAGGCCGCATGATCTTTCTGG1750.12262716436945112No Hit
CGTTGGGAGGAGTTTCCATGTCTTAGAAGGGCTTCATCATTTTCTTGCTGAA1740.12192643771591138No Hit
GCGCTACGGGACTTAAGTAAAATAAAGGCACTAATAAAGATAAGAGCAGAAA1740.12192643771591138No Hit
AGCTACGGGAACCAAACTGACTTACTGAGTCTTAGTGGAAAAACACTTTGTG1740.12192643771591138No Hit
CGTCAAGGGATTCCTCCTCCCCCATCATAACCATATTCAGCACCCATTTTTA1730.12122571106237168No Hit
ACGCCAGGGTTATGATACTGATTTTCCTTTAATGTTAATATGCCAGAAAGCA1720.12052498440883197No Hit
CATTGCGGGACTTACCTAAATCCTGGTAATAACAAATGGTTTTTGTAAAGTG1720.12052498440883197No Hit
CTCAACGGGATTAGCAAGTCAACAATGGCTTAGAATGATACAAACACTACAT1710.11982425775529223No Hit
CGACACGGGATTGTGCCCGGTCGGAGTAAACACAGAATGCTATCGAATCTAT1710.11982425775529223No Hit
ATACAAGGGTTATATGGCAAATAGCATCATCTTGAATTTTGTGAAATTATTT1710.11982425775529223No Hit
TTCCTAGGGAACTTGAAGGACTGTAGGAGGTAGAGGAACATGGAAAATTGAA1700.11912353110175253No Hit
TGCAGGGAATCATCTGTGTTTGTTTATTAAACATATGTCACTTTGAAATGCT1700.11912353110175253No Hit
ACAGGTGGGGAGTCTTCCAGGTGTGACAATCAGGATATAGAAAAACAAACGT1700.11912353110175253No Hit
GTATTCGGGGCCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCT1700.11912353110175253No Hit
TAACACGGGAACCTTGCTAAACTCTCCTGTTAGACCTGGTAAATCTCTGATA1690.11842280444821279No Hit
ATCCTGGGGTAAACAGTCATGTAACCCCTTTCAACTGTGTGCTTTTCCTAAT1690.11842280444821279No Hit
AGGAAAGGGAACCCTGATGATGATGTCAAGGGTCTTCTTCTGCTGTGTGGGC1690.11842280444821279No Hit
CGTTTTGGGGTAGGTCCTCTTGGGATTGCTAGTTTGTTCTTGCAGTCCATCT1690.11842280444821279No Hit
ATCTCTGGGAGAATCAGAAGACCTACTGCTGTTTGCACCTTAAAAAACTAAA1690.11842280444821279No Hit
TTAATGGGTATTCACATCTCTTTTTAAGACGAAGTATGCAAACATTTGTTTC1680.11772207779467307No Hit
CTTAACGGGTTCTCCCTGAAAAATACAAGGGCTGTTGGTGAGAGCAGACTTG1680.11772207779467307No Hit
ACCAGCGGGCTCTAGCAGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAG1680.11772207779467307No Hit
CCGAACGGGCCAGAGAAGGTGGTGCCACCTCCTCTTGTAGATAAATCTGCTG1680.11772207779467307No Hit
AGAAGCGGGATTTCCTTTCCTTTCCTTTGTAAAACATGCTCAGCAAACTGCA1680.11772207779467307No Hit
CTTTCAGTCTGCTAAACCAAAACCAGCAAAGAAAACAAATCCCTTTCCTCTC1670.11702135114113335No Hit
GATAATGGGGCCCGCGGGGGGGACCATGTGCCTGTGTCCCACGAGCAGCCGA1670.11702135114113335No Hit
TTCCACGGGGAGGAAATCAATCTCATTTACAATAACATCAAAAAATTGAAAT1670.11702135114113335No Hit
CATCAAGGGGATTTGTTGGAGTATGATATTTGCTGACAGCACTATATACTGG1670.11702135114113335No Hit
GTTGCTGGGAAGGTGTTAGCTGCATTCACAACCAGTCTAATCATTTGTCTCA1670.11702135114113335No Hit
AATCAGGGGAGCCTTTGAGAACCTGTATAAGAATACGTAAGTAATCCAGAGC1660.11632062448759363No Hit
AATAACGGGCTGCCCCAGTTTTGGTACTGAAAAATCTCATGTCCTGGGAAAC1660.11632062448759363No Hit
GGTAGTGGGATGTCTATCAGAAGAAACATCTGGTTGAAATTTTGTCATACCA1650.11561989783405392No Hit
AGCTAGTGGGATTATACTAGAAGAAGAAACCAGATCACTCCACCCCTCATCA1650.11561989783405392No Hit
CTTTATGGGAAGTGAGGGTACATACAGCATTTCACTGTGTTTTTAGTGTTGA1650.11561989783405392No Hit
GAGCAAGGGATTGAGTGTTGGTATTGGAGTATAGCATATCTATTATTCTGCT1650.11561989783405392No Hit
TCTGACGGGAGTTTCTCACTCTGTTGCTTAGTCTGGGATGCAGTGGCACAAT1650.11561989783405392No Hit
GACGCTGGGCCCAAATCCATATTCATGGAAGAATTTATAGTTGAAGCTCTCC1640.11491917118051419No Hit
GACACTACATTAGGAATTGGATTGTTTGGGTTTGCTTTGTGTTTTTTGAGGT1640.11491917118051419No Hit
GTTCTTGGGCGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCCT1640.11491917118051419No Hit
ACTGCAGGGATCATAGCAGGGATATATGCTAATGTGGAGAGCAGATAAAAAG1630.11421844452697448No Hit
GTAGTAGGGAAGACACAGGCACACAGAGACTTATTCAAAAGATCATAGAACA1630.11421844452697448No Hit
GAAACCGGGTGAGACATGCATTTTCACGTGTGGCCAATTGCACAGATGCGCA1630.11421844452697448No Hit
AAGAGGGGTTGCAAGTATTCAACTCTTCCTTTGTAGGGCAAAAGCAGCCATA1630.11421844452697448No Hit
TTTCGAGGGCAGGGCAATGAGAAAATTAGATGTGTAATATATCTTTCATCAA1630.11421844452697448No Hit
CCATAGGGGTAACACTATTTCGGCTGGTCAAGAGGATTTCCCATCTGTCCTG1620.11351771787343475No Hit
ATAAGAGGGTGATATCCAGATATGCGTACTTCAGCTAATATTTTATAGCCTT1620.11351771787343475No Hit
TATTGGGGGAGTACGCAGCCGCTTAGCGACTCTCGCGTTGCCCCCTGGGTGG1620.11351771787343475No Hit
CTTCCTGGGGCGTTAGCAGCCATTGAGGACTTCTTCCTAGAGCATGAAGCTC1620.11351771787343475No Hit
CTGTTAGGGGCATTCAGAAGACTTTTATCAAACTTATTAACCTATTTTCCTT1620.11351771787343475No Hit
CGGCTAGGGCACCTAACCTCTTATTGAAAGTAATTCAAAGTTATTTTCAAGC1620.11351771787343475No Hit
TATGCCAGGGCCAGGGGATAAACTCTGACTTAGTCTTGGAGTGGCAGATACA1620.11351771787343475No Hit
TGAACGGGACTGACCTTTCAGGCAATGGCATATGATCTACTACCAATTGAGA1620.11351771787343475No Hit
TATTGCGGGTGTCTTTGGAATACTAATACAAAAATTGTGTTAATGCTACTTT1610.11281699121989504No Hit
ACTCCAGGGGTTTTATAAATTCATATTTACTGTCCTGATCTAGCCACTATCA1610.11281699121989504No Hit
TACTTAGGGACAGGACGTATTCTTCCATCGTCTTATATATAACAAGCTGTTT1610.11281699121989504No Hit
CCATATGGGATGATTAAATTAGGACATAAGAGAAACTCAGCAGATCAGGGGG1610.11281699121989504No Hit
TGGCTCGGGTTGATTGAAGTATATTGTCATATAATAAAGTGCCTGTTATTTA1600.11211626456635532No Hit
GACACCGGGTGATGCGGGGCTGTGAATAAAAGGCTTACATTTGTTCCTGCAT1600.11211626456635532No Hit
CGGCGGGCAATGGCTGCAGAATACACATTCTTCTCCTCAGCACATGGATAAG1590.11141553791281558No Hit
GGACCCTCAATACCTACTTAACCTCATCTATTTCCGTTTGTCCTTTTGCCAG1590.11141553791281558No Hit
AATTCCGGGGTATTTGTCATGTATAACACAATATTTTGAAGTACAGTCCTGT1580.11071481125927587No Hit
AGTGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1580.11071481125927587No Hit
TTTGCCGGGGTGGCTTTGCAACCATTTTCATTTGTTTGTTTTCCTTTTATTT1580.11071481125927587No Hit
GCTATGGGGGGGACATTTTAGAGATTAGGATCCCCAGACAAGTAGTGATGTT1580.11071481125927587No Hit
TGCGCCGGGACCTGGTTAAATACTGTCTTAAAGCTCATACAAAACAAATAGG1570.11001408460573614No Hit
AAGCACGGGAAGAATTGTTTAGGTAAAAGGTTTTTAACTACTTAAAAGAGAT1570.11001408460573614No Hit
CACTAAGGGTACTAAAGCAAAAATAATTTAGGGTCTTATCCTGGGTAAAATA1560.10931335795219643No Hit
CTAAGCGGGCTGCCATGTCACCCTTCTGATTACAGCCACCTTTTGGGTTTCA1550.1086126312986567No Hit
ACTCGCGGGCGATCTCACAAGGGGAGACCAAACAACCCCATCAAAAAGTGGG1540.10791190464511699No Hit
GTCGCAGGGGTTTGTATGATTTTTGTTTTCAATACATGTGATGGGTTACTGT1540.10791190464511699No Hit
ACAGCGGGCCGATCTACGTTGGGGGTGCTTCTTTCTTTGGGAATATTTGAAT1540.10791190464511699No Hit
GTCCTTGGGTATGCAACACATCATAAGCCGCTAAAATAAATCTGTTGACTTT1540.10791190464511699No Hit
GCCTCAGGGCAGCTAATGTAATACACCATGTCAAAAGAATGAAAACAAAAAC1530.10721117799157727No Hit
CTGATTTCCCTAGCAATGATTCTGTTTCCATTGATTCAAATTACTAAAAATT1530.10721117799157727No Hit
TATGTGGGTCATTGGCTTAGTAAGAAAGGAAGCTATTAGGAGTTTGAAAAGA1530.10721117799157727No Hit
ACACACGGGATTCAACCTGAGAAAACTGATTAAAAAATTAGTTTAAATTTGC1530.10721117799157727No Hit
GCCTCGGGCAGAGCTAATATTTTTAGAGTAACTGATCTGGATCAATGATCTA1520.10651045133803755No Hit
CCAAAGGGGGAGAGAAAGCATTTTCCAAGCCAGGTTCACTTGGCTGCAAAGC1520.10651045133803755No Hit
TCTACTGGGACCTCAGAGGCAGAGGAACACCACCAGCTCTTCGATCTGATTG1520.10651045133803755No Hit
TCTTATGGGAGGAGAAGTTTGGAATGCACTTAATAGCTGGTTTTTACACCTT1510.10580972468449783No Hit
ACTACGGGGAAGAATGGTTCTAATTCACAGAGGTCTATTAGGCTGTGTTTAC1500.10510899803095809No Hit
GCGTATGGGAGTGTGGTGATTCCTCAAGGATCTAGAACCAGAAATACCATTT1500.10510899803095809No Hit
TTAGGCGGGGCTGGAGGTGCATTTTAAGTTTTAATAATAAGTGCTAGAATGA1500.10510899803095809No Hit
TATTTAGGGGAGACAACCACTGCTCACAGTTTGATGTATATCCTTCCAGAGA1490.10440827137741839No Hit
GTTCAAGGGAGAAAACATGGGTGTTTCCTTAAGCACATCCTTGGATTCTAGT1490.10440827137741839No Hit
CCACCTGGGGTGAGTGGATTAGAAATTTTGATAAGTATATTTCTTCCAATTT1490.10440827137741839No Hit
TATACTGGGAGTTAATCTTGGAAGATTCTTAATTTTTAAAGTCATTATTGAG1480.10370754472387865No Hit
TTCACCGGGACTTGGGAACGAGAATAAAAAACAAGCCAAAGCTTGTTAGCAG1480.10370754472387865No Hit
GTGATAGGGCAAGGACTTTTTGCATTCACAACTTGGCTAACAGCATAAGAGG1480.10370754472387865No Hit
TGGTACGGGACAGCTACCTGGGTAAGGCCCAAGATGGCTGTCTTCGCCTTAG1480.10370754472387865No Hit
AGTCTGGGCTCCCAGCTCCCTGCATTTGTGCCTTCTCTTTTAGGAATCATTG1480.10370754472387865No Hit
CGTCTAGGGATAGCACTTTTGTAGCATTTCACACATAGCAACTAATTTAATC1480.10370754472387865No Hit
CAGATAGGGTGGGTTTGTCCAGCTCAGGTAGCTCTAGGAGCACCATCCTGCC1480.10370754472387865No Hit
GAGATAGGGCTTACCCCATATTTACTTATTCTGATGTGCTTTTCAAACAAAA1470.10300681807033894No Hit
TGCTTTGGGGTATAGCAAAACATGAAAAGCACTGCATTACTGTGATAAAAGT1470.10300681807033894No Hit
GATACTACCATTCCTATCCAGCCATTTCTTGTCATTTCACCCCAATAAGCTG1470.10300681807033894No Hit
ATTGGTGGGAAGAGTGCAATTTTTAAAATCTAGAAGTTAGGGGTTTTGTTGG1470.10300681807033894No Hit
CTGCAGGGGGAGCAATATAGTGAGAACCCCGTCTCTATACAGACATAGATAA1470.10300681807033894No Hit
TGAAACGGGTACGTGGAATATCTTTTTCCACCCTACCACTTTCAATCTGCTT1460.10230609141679922No Hit
ATGAATGGGCTAGTTGTTTCTTGAAATCAAGCTGGCACGTGTGACTCCAGGA1460.10230609141679922No Hit
ACAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA1450.1016053647632595No Hit
CTAATAGGGATAATAACCAAGAGCTCTATGAGTCTTGTTTTTGGTTTTTAGT1450.1016053647632595No Hit
CTTAAAGGGCAGCATACTGAGGCGAGTTTAACACTGAATTCTATAAAAACTC1450.1016053647632595No Hit
TATTGTGGGAGAGTGGAATGGATTGGAACGGGGAGGTGTGAATAGCAGGTCA1450.1016053647632595No Hit
GTTATGGGGCTCACATTATGGCCTGGGAATTGCCAATATTATATTTCCTGAT1450.1016053647632595No Hit
TGAACAGGGAGCTCACTGCAACCTCCGCCTCCCAGTTCAAGTGATTCTCCTG1440.10090463810971978No Hit
TATTATGGGCAGACTGAATGGTCATGCTCAGTGCAAAAAGCTGTTTTGCTGT1440.10090463810971978No Hit
CCCAGGGCATATAAAGAAATAAAAAGAAGTCTCAGTTGCAGCTATTTGTCAA1440.10090463810971978No Hit
GTGGTGGGGGAGGTTGATTTGGAGCAGGTTACTTTTTTCATCTAAGATGTTT1440.10090463810971978No Hit
GTTGTAGGGGTAACTGCTGTAGCTGAAGCTACTGGAAGATTTGACTGAGTTG1430.10020391145618004No Hit
AGTCAGGGCACAATGGGATACGGACGAGCTGTAGTAAACTATGATTTGTCTG1430.10020391145618004No Hit
TTGATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1430.10020391145618004No Hit
ATGCCTGGGGCTGTGTGATGAAGTGACAGGGAGGGCCGGAGTCAGCAGGTGC1430.10020391145618004No Hit
CACCGAGGGAACAAAGCAAAACCTATCTTGGCAAAAAAAGGAAGTGAGTCAG1430.10020391145618004No Hit
TGCTCTGGGGATACTGGCAAGCCCTCTGGGTGTCCAGATCTCATGCAAACAC1430.10020391145618004No Hit
AAGATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1430.10020391145618004No Hit
TAGCTTGGGCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTA1430.10020391145618004No Hit
AACACAGGGATTGTCAGCATAAAGCCCAAACTCCTTGGTATGGTTTGTAGGT1430.10020391145618004No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)