FastQCFastQC Report
Sat 31 Dec 2022
SRR5846416.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846416.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48766
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC26065.3438871344789405No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT21994.509289258909896No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT6661.3657056145675266No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4991.0232539064102038No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2340.47984251322642824No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT2060.42242546036172746No Hit
GAACGAGGGTGAAAAGTAAAATGGACTACTTTTGTAACTTACCTGTTTGTTA2060.42242546036172746No Hit
GACATAGGGCTGGATGTGGACATTTGGAGCGCTTTCAGGCCTATGGTGAAAA2020.41422302423819873No Hit
AACCTTGGGATCTACAGACACGCATAAAGTAAACACAACTCTTGGAGGGATC2010.41217241520731657No Hit
TTTTTTGGGATGGAAGGCCAGTGAAGAAGCAAAGGAAGACATGAAAATTGAC1920.393716933929377No Hit
GTCCTAGGGATAATTATGAATTTTAGAAAAATGTATGTTCTTAAATATTTTA1890.3875651068367305No Hit
GAGAATGGGGATGAACTCTCACCTAGTAGAATGGCTAAAATCCAAAACACTG1880.38551449780584834No Hit
GGTAAAGGGATTTGAGGAAGTGCTCAACCACATTTCATTCTTCTGGAGTAGA1850.37936267071320184No Hit
CTTACGACGGGTGTCTTTAAAGCATAATATCTCTTAGACTTGACGGTTTGAG1720.3527047533117336No Hit
TGCGCTGGGTCTTCTTTCATCTCCTCAAAGGAATGAAGAAATGGTGGTGGGC1720.3527047533117336No Hit
AGGAGGGCAGTTTTCCTTAAATAAGTCAAAGATATGTTTACATTGTTATAAT1700.34860353524996923No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1700.34860353524996923No Hit
ATCTTGGGGACTCCAAAGGGGGAAAGACCCTTAGAAAGCCATCAGATCTCCT1670.34245170815732273No Hit
CACATAGGGTCCCCGACTCCCTTCTTTATGGCGTCGCTCCTGTGCTGTGGGC1660.34040109912644057No Hit
CAGAGCACAGGAAGCAGCTCAGCCCAATTATAGAACCTGACACCAAGCAAAG1630.334249272033794No Hit
GCATTGGGGATTATTAGGCAGAAATGCACTTGTTTAAATCATAGAAGTAATT1610.3301480539720297No Hit
AGACATGGGCTCATTCAAGAGTATGTGCAGGCATGCTTATGATCGTAGGAAC1610.3301480539720297No Hit
AATACAGGAAGAGTACATGGGTCTCTAACTACCATGAACAAGTTCCAGTATT1600.3280974449411475No Hit
CGAACCGGGCTCCTTTCCATCTAGCACAGCATCTTTGTCTCTGTTCTGTCTC1580.3239962268793832No Hit
GCCAATGGGGGCAGAGGGCCGATCCAGTTCTTTTCGGCTGGAATGGGGAATT1560.31989500881761884No Hit
CAACGAGGGTGAAGCATAGTCCTCATGCTTAATAAACTGACTGAAATCGTAG1550.3178443997867366No Hit
CAACATGGGCACTGATGAACAGTTTCTGGTGCAGGATCAGTTCCCAGTGACG1540.31579379075585445No Hit
ACGCGGGATGATATTTAAAGAAATATTTACTTTTCAATTTATAAATATACAT1510.30964196366320795No Hit
GATCATGGGTGGACTCTGGCGAAGAGCTTCACGGCGTCCTGCCCGGTCAGCG1480.30349013657056145No Hit
TGTGACTGGGGAAGTTTTGTTTTAGTTTTGATAATAAATTCTTTGGAACTTT1460.2993889185087971No Hit
TCACTAGGGTGGTGGAATTTTATTTTCTCAAATGTTTTCAGCATTTAAAATT1450.29733830947791495No Hit
GCTGCTGGGGCACATTTAACCCAAAAGAAAAACAAAAGGCATAAAATGAGAT1430.2932370914161506No Hit
AATTGGGGACAGTTGTGATGGAACACAAGCACTGTATATAAGACAGGTGCTA1410.28913587335438623No Hit
GACCATGGGTTATCAGGGAAATGCAAATCAAAACCACACTGAGATATCATCT1380.28298404626173973No Hit
ATTTCGGGGGAACCCATGTTTGTCACCAAGGAGCTTTCCAGGGCACATGTCA1360.2788828281999754No Hit
GCGTGAGGGGTCAGAAGGCTGGGCAGCCATGGCGTCCTATTTCGATGAACAC1360.2788828281999754No Hit
ATAGTGGGTCCCACTTACATTAACTAAATGAGGCATGAGCCACCATGCCTGG1340.274781610138211No Hit
CACCGTGGGAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAG1340.274781610138211No Hit
AACAGAGGGTGTCTGGGAAACAGGATACACACAGATATACACTTATCTTATG1330.2727310011073289No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1320.27068039207644673No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1310.26862978304556456No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1300.26657917401468234No Hit
TCTGCTGGGGTTTACCCCAAGTCACAGTTAGGACGGTAGATGGTGAGATCGC1300.26657917401468234No Hit
GTAATGGGGTACTCCCCAAACTAGAAACAAATTGATAAAAGCAGGTTAATAA1260.2583767378911537No Hit
TTTATTGGGTGGATGGGCTGAAGAAGAGAGACAAAAAGAACAAAACTAAGAA1260.2583767378911537No Hit
CATCAGGGGTAGTGACCGAGACTAAGAGGATTGTTTGACTTAATCAATATTA1210.2481236927367428No Hit
TTTGCGGGTAACCTTCCCCAGAAACAACAGTGATTGCGATTGTTTTCTAGAA1180.2419718656440963No Hit
CGCTACGGGGCCGGCTATGCCCCTGTATTGGATTGCCACACGGCTCACATTG1160.23787064758233195No Hit
TGTGGGGGCTGTGATTCCTGAATCTACAATAATGAGAACTCTACATAGTGGA1150.23582003855144978No Hit
TGACGACGGGCTCTTAAGGCTGTCGAAGACATGTTGGAAACATTGCAGATCA1140.23376942952056762No Hit
CAGACAGGGATCTCTCTTCGTATTACTTATATACTCCTCTTCTGATTTCAGG1140.23376942952056762No Hit
CATGCTGGGATCAAAGAACTGAAACTCACCAAATCACTGCATCCAGACAATG1130.23171882048968542No Hit
CAATACGGGTGTGTCCCAAGCATCTGCTAATCAAATATTATGAGATTACTTG1130.23171882048968542No Hit
TTCTAAGGGAAGGTGAAATAAAAACAGAAGGACAAGAGCTCATAACCGTAAT1120.22966821145880326No Hit
ATGCGCGGCTTTCTTGTTAACGGGTATCTTTTGTTGGTGTGTTTTGCTCTTA1110.2276176024279211No Hit
GAATCTGGGACTTGGGCCCACGCTTCTTAACTGACCCACTCCTCCCGTTGGC1070.21941516630439242No Hit
TTTTTGGGGGATCTGTATCAGGGCAGGAGCCATGGAGAAGTGGCATCAAGAC1060.21736455727351026No Hit
AAAATAGGGATAATTTCTATTGGAGAATATCAATAACAAAGAAAAAAGAAAT1050.21531394824262803No Hit
CAGACAGGGTAGTTGGCCTCTGGTCTACAAAGATTTCATAACTCCTTGGAAA1050.21531394824262803No Hit
TCACTGCGGGGAAATATAAGATATGCCAATACCTTGAGTTTTTCTGTAATTT1050.21531394824262803No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG1020.20916212114998156No Hit
CCGTTTGGGGTTCATGGATTCTAAAATGAACCTAAATACGTGGAGAATTTCT1020.20916212114998156No Hit
ATTCGGGATTTGAGCATAGAACAAACCTCATAGTTTCTGTTGAGATTTTATT1000.2050609030882172No Hit
TGGGGGGTATTGCTGTTGTTGTCATCGTTAACTGTGGGCATGGAATGCGTGA980.20095968502645287No Hit
TAAACAGGGTACCAGCCCAACCAACATGGAGAAACCCCGTCTCTACTAAAAA980.20095968502645287No Hit
GCAATAGGGGTCTCCTGTTTCTTTTCAAAGAGGAAGAAGAGTTTACAACAGA980.20095968502645287No Hit
TCTTTAGGGGCCCCAAGCCATTCATGAGGGATCTGCCTCCATGACCCAAACT970.19890907599557067No Hit
GCAGCTGTACTGCTCCCATTGTGCGTTCCTTCCCCTCTTCTCTGTGTTGTCC930.190706639872042No Hit
GGCCATGGGGGGCTCAAATTCTCTACCTCTTCAGGGCTAATACTTTTAACTG850.17430176762498462No Hit
GACAGTGGGAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCA850.17430176762498462No Hit
TTGGGGGTGAATGAGATGATATTGAGAGAGATATGGGAAAGAAAAAAAAAGT850.17430176762498462No Hit
TACTATGGGCCGATCTAAGAAGGGTGATGTAGGGGGAAAAGCAGCAGCCTCG850.17430176762498462No Hit
GCATCCGGGTGGAGAAGTCAATGGTACCTACTATCAATTTGGCAAACATTAT840.17225115859410245No Hit
GATGAGGGGGGGCCAGGCATGGTGTCTCATGCCTGTAATCCCAGCACTTTGG830.17020054956322028No Hit
GGTTCAGGGATACAAATCCCGTATGGTTGTGTCATCCTCTCTTAATCATTTT830.17020054956322028No Hit
CTAATGGGGCACAATATTTTGTAAAAAGTACCCAGTAGCCCCATTTCATACA820.16814994053233812No Hit
AAGCGTGGGGTTCATATACTTTTTAATTTTATGTATTTTAAGATATATGTCA820.16814994053233812No Hit
GATAACGGGATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATT810.16609933150145592No Hit
TCGAATGGGACTTTCTCCATACATAACACTTTAGCATTCAAGAAACATAGGA800.16404872247057375No Hit
AACTCTGGGAGCACGAGGAGGGTCGGGACTCGGAGGAGGGGCCAAGGTGTGG800.16404872247057375No Hit
GGTTATGGGACTGCCTTCTTGGATTTTTTTCTGATCACCTCAGCCAGAAGTG800.16404872247057375No Hit
GGCTACGGGGGGGACAAGGATCTTAAACCCAAAGTACGAGAAATGTTTCTGT800.16404872247057375No Hit
TTTATTGGGATTGTCAAGCTAGCAAAAAAACTCTTGTTTTTTTAATTGGGAG780.15994750440880942No Hit
GCATTAGGGGTTTCTCAAAGTTTTCTATTTAGACCAGTACTCCTTTTCTCTA780.15994750440880942No Hit
AATCTAGGGATGGATTGGAAGGTGCTCACATGGCTTCGTTACACACTGTGGA770.15789689537792723No Hit
GTTTCTGGGAATTTTTGTTTGTTTGAAGATAGGTTAAATGGCTATATGATCT770.15789689537792723No Hit
GATTATGGGAGCAAGACCTCGTCTTTGTTTAGGGGAAAAAAAAGAAATTTAA770.15789689537792723No Hit
AAAACTGCGGCATCCAGCTTCATCTAGGTGGTCATGATTACCTGCATGCTTT750.1537956773161629No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATA740.15174506828528073No Hit
TGCTTAGGGATGGACGCCTGGCAACCAAGTATCATACCGTTCCACTGCCACC730.14969445925439856No Hit
TTCACAGGGGGGTCCTCCAGTTTTTGGCACAGATGTTCCGGAGCCCAGGAAG720.1476438502235164No Hit
AACCCTGGGGCTCTTCCGATCTCAAATGGGCTACAGCTCACTTCTATCAGAG690.14149202313086987No Hit
TGGTACGGGTCTTTTTATTCAGTAATCATCAGTCTTTTCCAATGTTTGTTTA690.14149202313086987No Hit
AATATTGGGGTGGCCGGGCGTGGTGGCTCACGCCTATAATCCCAGCACTATG680.1394414140999877No Hit
ATAGCTGGGTGCCTAAAGAATTGGCCCTGACATTTGTTAATCAAGTTAACTG680.1394414140999877No Hit
GTTGCTGGGAGGTCGCGGCGACATGGCCAAACGTACCAAGAAAGTCGGGATC680.1394414140999877No Hit
GCCAAAGGGATCCTGGAAGGCAGCAAGGTTTTGTGGATCTAGATTCATTAGG660.13534019603822336No Hit
TACATTGGGTCGCGCCACTGCACTCCAGCCTGGGTGACAGAGCGAGACTCCG660.13534019603822336No Hit
TCTGGCAGTGGCTACAGGTGTGAGTCAACATGCTCGGCCTGTTACACACACA660.13534019603822336No Hit
GATGACGGGGGGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGTGCCATC660.13534019603822336No Hit
AAAGACGGGGGAAAGACAAATCAGTTATAACAAGCAATATTAATTGTGCACT650.13328958700734117No Hit
ACTGCCGGGTGCTTTATTGCTTAAAAATGCTGACACAGAGACATGAAGTGGG640.131238977976459No Hit
TGAAGGGGGTTATGAAAGATAGCACAAAGTTTTTCTGAAACTACATTAAAAA640.131238977976459No Hit
CTCGTCGGGATCCCAACGAGCTGCCTCATGTTCTTTATTTTCTAAGGGCAGG620.12713775991469467No Hit
ATTAAGGGTAAAATAAATGGAATGTGCTACGTGATTCCTAGGACTGATTTAA610.12508715088381248No Hit
ATATCCGGGGGTTGAATACGGGAACTGCCACGGGGCAAGCTGTCAATGGTGG580.11893532379116598No Hit
AGTAGCGGGTACTGACTTGTTGCCATTAAGTGAACTTGACTTCTTATGTGTG570.11688471476028381No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCG570.11688471476028381No Hit
ATCTGTGGCGTGCCACTGCACTCCAGCCTGAGCAACAGAGCAAGACTCTATC560.11483410572940163No Hit
TTATGCCGGGTGATATAATGTAACTATCACAACCTTACAGTAAACATCATGC560.11483410572940163No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC560.11483410572940163No Hit
TATTAAGGGGACGCTCTTCCGATCTTAGGCGAGGGTTATGATAATGGACTTT560.11483410572940163No Hit
TTTATTGGGTACGTTCAAAATTTTACTTTTGCTTTAGCAAAAGAAAGTGAAG550.11278349669851948No Hit
AGTCAAGGGACAAATCTTCATACAGAGTAGGATAAAGAACTATTCAGATATA550.11278349669851948No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTACCTCGTATGC540.11073288766763728No Hit
TCCACCGGTTCCTTTTCCCATTTTCACTGTCCTGTTTTCTCTTTCTTATCAT530.10868227863675513No Hit
GTACACGGGCACGACGCTCTTCCGTTCTACATAAGGGATAGGTTTGGTCCTA530.10868227863675513No Hit
GCCTGCCTTGGCCTCCCAAAGTGTTAGGATTACAGACGTGAGCCATCACGCC530.10868227863675513No Hit
GATGATGGGATGGGTAAATTATTTAAACTCACTTAATTAAGGAAAATATTTT520.10663166960587295No Hit
AGTGCAGGGTCCACCCGAGTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTG520.10663166960587295No Hit
CAGCGAGGGAGGGGGCCGGGTGTGGTGGCTCACACCTGTAATCCCAGCACTT510.10458106057499078No Hit
GTTCTGGGGGATCACAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGA500.1025304515441086No Hit
TCCTCTAGGCAGTTGGGTTATTCTAGAAGCAGATGTGTTGGCTCTTTCTGAA500.1025304515441086No Hit
CTATCAGGGAACCACGTTATTACTAAAACAATGGAATTAAAGCCCTTAAATG500.1025304515441086No Hit
TACAAGGGGGCCGAGGCGGCGGATCACGAGGTCAGGAGATCGAGACCATCCT490.10047984251322643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)