FastQCFastQC Report
Sat 31 Dec 2022
SRR5846415.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846415.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47913
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC25685.35971448249953No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT20924.3662471563041345No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT6301.3148832258468475No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4931.0289483021309456No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2310.4821238494771774No Hit
GACATAGGGCTGGATGTGGACATTTGGAGCGCTTTCAGGCCTATGGTGAAAA2190.4570784546991422No Hit
GAGAATGGGGATGAACTCTCACCTAGTAGAATGGCTAAAATCCAAAACACTG2080.43412017615260995No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1980.4132490138375806No Hit
AATTGGGGACAGTTGTGATGGAACACAAGCACTGTATATAAGACAGGTGCTA1940.40490054891156885No Hit
GGTAAAGGGATTTGAGGAAGTGCTCAACCACATTTCATTCTTCTGGAGTAGA1930.402813432680066No Hit
GAACGAGGGTGAAAAGTAAAATGGACTACTTTTGTAACTTACCTGTTTGTTA1890.39446496775405426No Hit
GTCCTAGGGATAATTATGAATTTTAGAAAAATGTATGTTCTTAAATATTTTA1880.3923778515225513No Hit
AACCTTGGGATCTACAGACACGCATAAAGTAAACACAACTCTTGGAGGGATC1870.39029073529104835No Hit
CACATAGGGTCCCCGACTCCCTTCTTTATGGCGTCGCTCCTGTGCTGTGGGC1850.3861165028280425No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1820.3798551541335337No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1780.37150668920752195No Hit
AGGAGGGCAGTTTTCCTTAAATAAGTCAAAGATATGTTTACATTGTTATAAT1770.369419572976019No Hit
CAGAGCACAGGAAGCAGCTCAGCCCAATTATAGAACCTGACACCAAGCAAAG1750.36524534051301316No Hit
CGAACCGGGCTCCTTTCCATCTAGCACAGCATCTTTGTCTCTGTTCTGTCTC1720.3589839918185044No Hit
GCATTGGGGATTATTAGGCAGAAATGCACTTGTTTAAATCATAGAAGTAATT1690.3527226431239956No Hit
AGACATGGGCTCATTCAAGAGTATGTGCAGGCATGCTTATGATCGTAGGAAC1680.3506355268924926No Hit
CAACATGGGCACTGATGAACAGTTTCTGGTGCAGGATCAGTTCCCAGTGACG1640.3422870619664809No Hit
CTTACGACGGGTGTCTTTAAAGCATAATATCTCTTAGACTTGACGGTTTGAG1630.34019994573497797No Hit
CAACGAGGGTGAAGCATAGTCCTCATGCTTAATAAACTGACTGAAATCGTAG1600.3339385970404692No Hit
TCACTAGGGTGGTGGAATTTTATTTTCTCAAATGTTTTCAGCATTTAAAATT1570.3276772483459604No Hit
GATCATGGGTGGACTCTGGCGAAGAGCTTCACGGCGTCCTGCCCGGTCAGCG1560.3255901321144575No Hit
ATTTCGGGGGAACCCATGTTTGTCACCAAGGAGCTTTCCAGGGCACATGTCA1550.32350301588295455No Hit
TGCGCTGGGTCTTCTTTCATCTCCTCAAAGGAATGAAGAAATGGTGGTGGGC1530.3193287834199487No Hit
GCGTGAGGGGTCAGAAGGCTGGGCAGCCATGGCGTCCTATTTCGATGAACAC1500.31306743472543985No Hit
GACCATGGGTTATCAGGGAAATGCAAATCAAAACCACACTGAGATATCATCT1490.3109803184939369No Hit
AATACAGGAAGAGTACATGGGTCTCTAACTACCATGAACAAGTTCCAGTATT1480.308893202262434No Hit
CGCTACGGGGCCGGCTATGCCCCTGTATTGGATTGCCACACGGCTCACATTG1470.30680608603093107No Hit
TTTTTTGGGATGGAAGGCCAGTGAAGAAGCAAAGGAAGACATGAAAATTGAC1460.30471896979942814No Hit
TCTGCTGGGGTTTACCCCAAGTCACAGTTAGGACGGTAGATGGTGAGATCGC1450.3026318535679252No Hit
GCTGCTGGGGCACATTTAACCCAAAAGAAAAACAAAAGGCATAAAATGAGAT1440.3005447373364223No Hit
ATAGTGGGTCCCACTTACATTAACTAAATGAGGCATGAGCCACCATGCCTGG1430.29845762110491936No Hit
ATCTTGGGGACTCCAAAGGGGGAAAGACCCTTAGAAAGCCATCAGATCTCCT1400.2921962724104105No Hit
GTAATGGGGTACTCCCCAAACTAGAAACAAATTGATAAAAGCAGGTTAATAA1380.2880220399474047No Hit
TTTATTGGGTGGATGGGCTGAAGAAGAGAGACAAAAAGAACAAAACTAAGAA1370.28593492371590173No Hit
TGTGGGGGCTGTGATTCCTGAATCTACAATAATGAGAACTCTACATAGTGGA1330.27758645878989No Hit
CACCGTGGGAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAG1320.2754993425583871No Hit
CAATACGGGTGTGTCCCAAGCATCTGCTAATCAAATATTATGAGATTACTTG1310.27341222632688417No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1300.2713251100953812No Hit
ACGCGGGATGATATTTAAAGAAATATTTACTTTTCAATTTATAAATATACAT1300.2713251100953812No Hit
TAAACAGGGTACCAGCCCAACCAACATGGAGAAACCCCGTCTCTACTAAAAA1290.2692379938638783No Hit
TGTGACTGGGGAAGTTTTGTTTTAGTTTTGATAATAAATTCTTTGGAACTTT1270.2650637614008724No Hit
GCCAATGGGGGCAGAGGGCCGATCCAGTTCTTTTCGGCTGGAATGGGGAATT1250.26088952893786654No Hit
AACAGAGGGTGTCTGGGAAACAGGATACACACAGATATACACTTATCTTATG1240.2588024127063636No Hit
GCAGCTGTACTGCTCCCATTGTGCGTTCCTTCCCCTCTTCTCTGTGTTGTCC1210.25254106401185483No Hit
GCATCCGGGTGGAGAAGTCAATGGTACCTACTATCAATTTGGCAAACATTAT1150.2400183666228372No Hit
GAATCTGGGACTTGGGCCCACGCTTCTTAACTGACCCACTCCTCCCGTTGGC1120.23375701792832843No Hit
GCAATAGGGGTCTCCTGTTTCTTTTCAAAGAGGAAGAAGAGTTTACAACAGA1110.2316699016968255No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1100.22958278546532257No Hit
TTGGGGGTGAATGAGATGATATTGAGAGAGATATGGGAAAGAAAAAAAAAGT1100.22958278546532257No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG1070.22332143677081376No Hit
TTCTAAGGGAAGGTGAAATAAAAACAGAAGGACAAGAGCTCATAACCGTAAT1070.22332143677081376No Hit
CATGCTGGGATCAAAGAACTGAAACTCACCAAATCACTGCATCCAGACAATG1050.21914720430780793No Hit
CATCAGGGGTAGTGACCGAGACTAAGAGGATTGTTTGACTTAATCAATATTA1040.21706008807630497No Hit
ATTCGGGATTTGAGCATAGAACAAACCTCATAGTTTCTGTTGAGATTTTATT1020.21288585561329912No Hit
TGACGACGGGCTCTTAAGGCTGTCGAAGACATGTTGGAAACATTGCAGATCA1020.21288585561329912No Hit
GTTGCTGGGAGGTCGCGGCGACATGGCCAAACGTACCAAGAAAGTCGGGATC1020.21288585561329912No Hit
TGGGGGGTATTGCTGTTGTTGTCATCGTTAACTGTGGGCATGGAATGCGTGA1000.20871162315029324No Hit
TCACTGCGGGGAAATATAAGATATGCCAATACCTTGAGTTTTTCTGTAATTT1000.20871162315029324No Hit
TTTTTGGGGGATCTGTATCAGGGCAGGAGCCATGGAGAAGTGGCATCAAGAC1000.20871162315029324No Hit
GACAGTGGGAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCA980.20453739068728738No Hit
TTTGCGGGTAACCTTCCCCAGAAACAACAGTGATTGCGATTGTTTTCTAGAA970.20245027445578442No Hit
GGTTCAGGGATACAAATCCCGTATGGTTGTGTCATCCTCTCTTAATCATTTT970.20245027445578442No Hit
CTAATGGGGCACAATATTTTGTAAAAAGTACCCAGTAGCCCCATTTCATACA960.20036315822428152No Hit
GATAACGGGATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATT920.19201469329826978No Hit
AAGCGTGGGGTTCATATACTTTTTAATTTTATGTATTTTAAGATATATGTCA910.18992757706676686No Hit
CAGACAGGGTAGTTGGCCTCTGGTCTACAAAGATTTCATAACTCCTTGGAAA890.18575334460376097No Hit
CCGTTTGGGGTTCATGGATTCTAAAATGAACCTAAATACGTGGAGAATTTCT890.18575334460376097No Hit
ATGCGCGGCTTTCTTGTTAACGGGTATCTTTTGTTGGTGTGTTTTGCTCTTA870.18157911214075512No Hit
TCGAATGGGACTTTCTCCATACATAACACTTTAGCATTCAAGAAACATAGGA840.1753177634462463No Hit
GATTATGGGAGCAAGACCTCGTCTTTGTTTAGGGGAAAAAAAAGAAATTTAA840.1753177634462463No Hit
TTTATTGGGTACGTTCAAAATTTTACTTTTGCTTTAGCAAAAGAAAGTGAAG810.16905641475173752No Hit
TGAAGGGGGTTATGAAAGATAGCACAAAGTTTTTCTGAAACTACATTAAAAA800.1669692985202346No Hit
AAAATAGGGATAATTTCTATTGGAGAATATCAATAACAAAGAAAAAAGAAAT800.1669692985202346No Hit
CAGACAGGGATCTCTCTTCGTATTACTTATATACTCCTCTTCTGATTTCAGG780.16279506605722874No Hit
ACTGCCGGGTGCTTTATTGCTTAAAAATGCTGACACAGAGACATGAAGTGGG760.15862083359422285No Hit
TTTATTGGGATTGTCAAGCTAGCAAAAAAACTCTTGTTTTTTTAATTGGGAG760.15862083359422285No Hit
GTTTCTGGGAATTTTTGTTTGTTTGAAGATAGGTTAAATGGCTATATGATCT740.154446601131217No Hit
AAAACTGCGGCATCCAGCTTCATCTAGGTGGTCATGATTACCTGCATGCTTT740.154446601131217No Hit
AATCTAGGGATGGATTGGAAGGTGCTCACATGGCTTCGTTACACACTGTGGA730.15235948489971407No Hit
TGCTTAGGGATGGACGCCTGGCAACCAAGTATCATACCGTTCCACTGCCACC720.15027236866821114No Hit
GATGAGGGGGGGCCAGGCATGGTGTCTCATGCCTGTAATCCCAGCACTTTGG720.15027236866821114No Hit
TACTATGGGCCGATCTAAGAAGGGTGATGTAGGGGGAAAAGCAGCAGCCTCG710.1481852524367082No Hit
ATAGCTGGGTGCCTAAAGAATTGGCCCTGACATTTGTTAATCAAGTTAACTG700.14609813620520526No Hit
GGCTACGGGGGGGACAAGGATCTTAAACCCAAAGTACGAGAAATGTTTCTGT700.14609813620520526No Hit
GTACACGGGCACGACGCTCTTCCGTTCTACATAAGGGATAGGTTTGGTCCTA680.1419239037421994No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATA670.13983678751069648No Hit
CTCGTCGGGATCCCAACGAGCTGCCTCATGTTCTTTATTTTCTAAGGGCAGG660.13774967127919355No Hit
AAAGACGGGGGAAAGACAAATCAGTTATAACAAGCAATATTAATTGTGCACT660.13774967127919355No Hit
GCATTAGGGGTTTCTCAAAGTTTTCTATTTAGACCAGTACTCCTTTTCTCTA660.13774967127919355No Hit
ATCTGTGGCGTGCCACTGCACTCCAGCCTGAGCAACAGAGCAAGACTCTATC650.1356625550476906No Hit
TCTTTAGGGGCCCCAAGCCATTCATGAGGGATCTGCCTCCATGACCCAAACT650.1356625550476906No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC640.13357543881618766No Hit
AACTCTGGGAGCACGAGGAGGGTCGGGACTCGGAGGAGGGGCCAAGGTGTGG640.13357543881618766No Hit
TTCACAGGGGGGTCCTCCAGTTTTTGGCACAGATGTTCCGGAGCCCAGGAAG640.13357543881618766No Hit
AATATTGGGGTGGCCGGGCGTGGTGGCTCACGCCTATAATCCCAGCACTATG630.13148832258468474No Hit
AACCCTGGGGCTCTTCCGATCTCAAATGGGCTACAGCTCACTTCTATCAGAG630.13148832258468474No Hit
GTTCTGGGGGATCACAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGA630.13148832258468474No Hit
TTATGCCGGGTGATATAATGTAACTATCACAACCTTACAGTAAACATCATGC620.1294012063531818No Hit
GGCCATGGGGGGCTCAAATTCTCTACCTCTTCAGGGCTAATACTTTTAACTG620.1294012063531818No Hit
GCTGCAGGGCATGTCTCTCATCTTCCATGAGGCTGTTTTGGGCTTCATTACG600.12522697389017595No Hit
AGTAGCGGGTACTGACTTGTTGCCATTAAGTGAACTTGACTTCTTATGTGTG600.12522697389017595No Hit
GGTTATGGGACTGCCTTCTTGGATTTTTTTCTGATCACCTCAGCCAGAAGTG600.12522697389017595No Hit
TTTGACGGGCAGTTGAAACCTGTATAACCCTGTTACAAAGCTGTGTTGTTGC600.12522697389017595No Hit
TCTGGCAGTGGCTACAGGTGTGAGTCAACATGCTCGGCCTGTTACACACACA590.12313985765867301No Hit
GATGACGGGGGGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGTGCCATC580.12105274142717008No Hit
GATGATGGGATGGGTAAATTATTTAAACTCACTTAATTAAGGAAAATATTTT570.11896562519566715No Hit
GCCTGCCTTGGCCTCCCAAAGTGTTAGGATTACAGACGTGAGCCATCACGCC570.11896562519566715No Hit
GCTTCAGGGAGCTAGAGGCCGCCACGCAAGTGAAGCCAGCAGAGGAGTGATA560.11687850896416421No Hit
ATATCCGGGGGTTGAATACGGGAACTGCCACGGGGCAAGCTGTCAATGGTGG550.11479139273266128No Hit
TCCTCTAGGCAGTTGGGTTATTCTAGAAGCAGATGTGTTGGCTCTTTCTGAA550.11479139273266128No Hit
ATTAAGGGTAAAATAAATGGAATGTGCTACGTGATTCCTAGGACTGATTTAA540.11270427650115836No Hit
GCCAAAGGGATCCTGGAAGGCAGCAAGGTTTTGTGGATCTAGATTCATTAGG530.11061716026965542No Hit
AGTCAAGGGACAAATCTTCATACAGAGTAGGATAAAGAACTATTCAGATATA530.11061716026965542No Hit
TGGTACGGGTCTTTTTATTCAGTAATCATCAGTCTTTTCCAATGTTTGTTTA530.11061716026965542No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC520.10853004403815249No Hit
ACATACGGGGTTCTGCCTCCCTGGTTCAATCGATTCTCCTGCTTCGGCCTCC520.10853004403815249No Hit
TATTAAGGGGACGCTCTTCCGATCTTAGGCGAGGGTTATGATAATGGACTTT500.10435581157514662No Hit
CAGCGAGGGAGGGGGCCGGGTGTGGTGGCTCACACCTGTAATCCCAGCACTT500.10435581157514662No Hit
ATGGTCGGGCCTGAAAGCAGTAACCAAGAGTTAAGATATCCCTAATGTTTTG480.10018157911214076No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCG480.10018157911214076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)