FastQCFastQC Report
Sat 31 Dec 2022
SRR5846404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111695
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT21671.9401047495411612No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC21361.9123505976095616No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT4300.3849769461479923No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA2940.26321679573839474No Hit
CACTCTGGGAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCT2010.1799543399435964No Hit
ATTCACGGGCAGCACTGAAATAGGTTGAGGGTTCCCTTCCCATTTACCTTGA1940.1736872733783965No Hit
TTTGTCGGGGATCTGAGAAGAGCCTCATTCAATAGTTTTAAATGTTAAAAGG1860.16652491158959665No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1810.16204843547159678No Hit
TACTCCGGGTCTGCACATGTACTCCAGAACTTAAAGTATAATTAAAAAACAG1760.15757195935359686No Hit
TTTAACGGGATGTCTGCATTACTCATTGCTAAAAATGTGTAGCACAGAACTG1750.15667666412999687No Hit
ATGTTTGGGCTGGGAAGGAGAACAACAATTATATTCCCCCATTTCAAGAAGG1740.15578136890639688No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC1710.15309548323559694No Hit
ATGAAAGGGAGATTCTAGCTCTGACACTTACTAGTGATTTTGAGTATGTTGT1710.15309548323559694No Hit
TTATCTGGGATGGCGTTAGCATCCATATGATCCATAGGCTGGTTGCTGGGAA1680.150409597564797No Hit
GCGTTTGGGTCCGATCTTCATGTGGGCGATCTCAAACCGGGTGTTCTTACAG1680.150409597564797No Hit
CCACAAGGGATTTTGACCCCACTTACAGATCAGAAAACTGAGGCAGATATCT1670.149514302341197No Hit
CGGCTGGGATGGATAATATGTCCAAAAATTTATAAAAATCAAATGGAAATTT1630.1459331214467971No Hit
CTTTGTGGAGACTGCACATCCGGACCTGCCCATGTCTCAAAACAAACACATG1620.1450378262231971No Hit
ATTCGAGGGCAAAGCCATTTATGTTTCTGGCTCAGCAAGCCTTTCAGTTCTT1610.14414253099959712No Hit
AGGCTGGGGGCCTTTTCAGGCGTGGCCTCCTTTCCATCTAGCACAGCATCTT1610.14414253099959712No Hit
TTGCAAGGGATTGAACGCAACAGGAGGGTATTTCAGCAAATTCACGACAAGG1600.14324723577599713No Hit
GTCAGCGGGTGCCCTGAATGACCATTGAGTCAATGATGAAATTAATATGGAA1600.14324723577599713No Hit
GAAAAAGGGACTAGGTGATCCAGTAAGTTATTTGATAAGGGGCATTTTCTTC1590.14235194055239714No Hit
TCAAAGGGCGATCTATTAAAGGGGAGATCACTAACTTTACACACACACACAC1560.13966605488159722No Hit
TGTACGGGGCATTTAATTTTCTCATAGATTTAAGTAAACAGATGTATTTTGC1540.13787546443439724No Hit
ATTAATGGGCGTAAAATTAATCATTTGTGACTTAACTTTGAATTTTTATAAT1530.13698016921079725No Hit
TGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAG1520.13608487398719726No Hit
TCCATTGGGAAGACTTTAAAACCTAAATGACTTTTGACATACAAACTCTTCT1520.13608487398719726No Hit
AAATCAGGGATCTTTCCAAATTTGCCATAACAGATTTAGGTTTCTTTCCTTT1510.1351895787635973No Hit
CGAACCGGGGATCCTGAGAGATGAGAGGCAGCCTAAATAGACCTCTTTCCTG1490.13339898831639735No Hit
GCTGTAGGGGTGGTCTCTTGAATTTTGTAAGTTCATCTGAGGAGAAAAGATT1470.13160839786919737No Hit
CAATCGGGGATATTTTAAGAGTTTTCTTTTTTCTATGATGCTTTTATTTGTG1450.1298178074219974No Hit
GTAACCGGGGCGAGCGTTGGTGCTGCTTGATAACCCATGCACGGACGAAACC1450.1298178074219974No Hit
AGCTTCGGGGACCTTTGAAATTTGCATGTGACCTCATCTAGCTATGAATTCT1440.12892251219839743No Hit
CAACAAGGGCTATCACAGTATCTATAACATAGTAAGAGCTTTATTCGGGTCT1440.12892251219839743No Hit
CACTTTGGGACTGTAAGTCTCAATTAACAGCAGAATCTCAGAGAAAAGCTGT1440.12892251219839743No Hit
ATGAGTGGGCAGTCTTTACTAATTTTAAATATTATTCTTTTTTTATATTTCT1430.12802721697479744No Hit
TACAACGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1430.12802721697479744No Hit
CTTTAGGGGAATGCCTTATTTTTGAATTCTTTAGATTTTTAAATTGGAGAAA1430.12802721697479744No Hit
TTGTTAGGGGGGCTGGGCGCCGAGTGGGACAGCGCTGGTGCGGAGACTGCTT1420.12713192175119745No Hit
CAGCCTGGGCGACAGAGCAAGACTCCATCTCAAAAACAAACAAACAAAAAAG1410.12623662652759748No Hit
ATCCAGGAGAAAATAAAGTAAATTATTTTAAAAAATTACCTATATTCCCAAC1410.12623662652759748No Hit
ACACTGGGACCACTACTCTATGCACTACTCGGCGCTGCCCGGTTCGCGGCCC1390.12444603608039752No Hit
AAAAGCGGGATAAGTGAAATGATGAGTTATTTGTCTTTATGTGCCTGCCTTA1390.12444603608039752No Hit
GACAATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG1380.12355074085679753No Hit
TTATCAGGGAGAGACGCCAGAGGTGCAGCTCCAGCAGCAATGGCAGTGACGG1370.12265544563319754No Hit
ACACGGGTGGGGAGAAAAAGCCTTTAGTGAATTTTCTTGAGGGTGATAAATC1370.12265544563319754No Hit
TTGGTGGGAGATCCAGTCACAGGTTGTGGCTCAGATACAGGCTCAGCAAAGT1360.12176015040959758No Hit
TTCTTAGGGACTATTAATTTTTGTTTGTGGAGGAGGGGAAAAGTGCTTTAAA1360.12176015040959758No Hit
CATTTTGGGACTTCATTTTCTAGTTGAAAAAAGTGTTGTTTTGCCTTTGCTC1360.12176015040959758No Hit
TTACGCGGGCTGGTACTGAATGCCGCTATCACCATGGGGATGGTTCTTCTAA1360.12176015040959758No Hit
TAGCTAGGGCGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTA1360.12176015040959758No Hit
GCGTTGGGGTCCAGTAGACTAAGATTGAGTGTTCTTTTTGTTCAGCAACTCT1350.12086485518599757No Hit
AACAGAGGGTTAACAACCGATGCAACATTAAAAATGTGTTAGTGTGCTGTGC1340.11996955996239761No Hit
TTAGTGGGGTGATGGTTACAATGTGCAGCCAGACACCGTGGCCCCAATCTGG1340.11996955996239761No Hit
AGAAATGGGATGTGCATTTTAAATGCTAGAACTTAAAAACCCACTGAAACAC1340.11996955996239761No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1340.11996955996239761No Hit
TAGTTTGGGGAGGCCAAGTGTGCGTCCAGGGGAACAGCACAGGTTAATGCGT1340.11996955996239761No Hit
TCAAATGGGGAACTGTATCGTCACACTATTTTTTAATTTCAGTAAGTTCTTC1330.11907426473879762No Hit
TGTCCAGGGCACCCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCC1330.11907426473879762No Hit
TTGTTAGGGCGATCTTGGAGAGGGATGACCCCAATACGCAAAACTAACCCCC1330.11907426473879762No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1330.11907426473879762No Hit
AGCTCTGGGTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTA1330.11907426473879762No Hit
TTCGATGGGCAGTCCACTCTGTCTTTAAAACATAGTGATTACAATATTTAGA1330.11907426473879762No Hit
CGCGACGGGGGGGTCAGCTGCCCCACTCCTGACTTTTTTATTTTTAATGGTC1330.11907426473879762No Hit
ACGCACGGGAATAAAGGAAAGGATCCAACAGAAAATTCATTAAGGAATCTTT1320.11817896951519763No Hit
ACGACGGGATATATAACGTGATATATATATATACACAAAGACACACACAGTG1320.11817896951519763No Hit
ATCATCGGGATAGGAGGTAATCACCAGACAACTGCGGAATGTGGAGCACTGA1310.11728367429159765No Hit
GATGAAGGGAACCTCTGTTGCTTAGAAAGCTTCCAGTGTTCTTAGATCTCTC1310.11728367429159765No Hit
GATTTTGGGAGGCTACAGTCTGTGCCATGTTGATGTACAGTTTCTGAAATTG1300.11638837906799766No Hit
TCGGTTGGGCTGGGAATCAGGAGCCTGGACACTGCCATCTCTAGAGCAGAGT1300.11638837906799766No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1290.1154930838443977No Hit
AAGTATGGGAACCATTTTATTAATATATAATGTCTTTGTCTCTTGTAACAAT1290.1154930838443977No Hit
TTTACTGGGTCCCAATTATAAATGAAACTGGCTTTCTTCAAAAGACCAGCTG1290.1154930838443977No Hit
TCAGCAGGGATATAGCAAAAAGTAATCATATCTGGCTAGATAGCCATTTCTC1290.1154930838443977No Hit
TCGACTGGGTGAGTGCATCTTGTATGTGCCTAATATTGATAGGATGAGATGT1280.11459778862079771No Hit
GTACATGGGACCTGCAGGAACAACATGGAGAGCTTTCTTTGGAATTCCTTAG1280.11459778862079771No Hit
TACGCAGGGATTCTTACTCTGTGATTAAGGTTTTTATGAGTAACCAAGGAAC1280.11459778862079771No Hit
GCAACTGGGTGATGAAAGTGGTGGACTTGAAATTTCCCGTTCATCTCCCAAA1270.11370249339719772No Hit
AACGCTGGGACCTCCTACTGTTGATTCAACTCAACAGCCTAACCCTTTGCCG1260.11280719817359774No Hit
CTTAAGGGTGCCGGTGTTTTAAGAAAGCCAGATGCCTGGATTGGACTCTGGG1260.11280719817359774No Hit
TCAGCGGGGCAATGAGTCATGTCTGCATGATTTCATTATGTAGTATTTCATT1260.11280719817359774No Hit
TTGGACGGGATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATTTCT1260.11280719817359774No Hit
CTTACGGGTATGCATCTGCAAGTCAGGAAGGCCAAAGATGACCACAAACCAC1260.11280719817359774No Hit
CAAGATGGGCTTGTGCCTTTCATGTAAGTTAAATTTGCTCATACAGCTTGAA1250.11191190294999775No Hit
GTACGAGGGATAACTATAAATTGCATACCCTAACGTAATTATGCATTAATTA1250.11191190294999775No Hit
GTCAGCGGGGCCTGTAAGTGTACATGTAGTGGTGTGGTGTTCTGTCTAGAGG1240.11101660772639779No Hit
CTCAGAGGAGATCTGTAGGGACTTGTACAACATTGTGGATGTGTAAACAGGC1230.1101213125027978No Hit
ACATTTGGGAAGAAGAAATGATCATTTTGGAACCAGACAAGAATCCAAAGAA1230.1101213125027978No Hit
TTGCTGGGGCAGCCATTTACTTGCTATTTTAATCAACATCAAAGACCAGATG1230.1101213125027978No Hit
TTATCCGGGGATCTTCACGTGGGAATGCCCCAACTAAATACTACCGTATGGC1220.10922601727919781No Hit
AGTAGAGGGGGCCAAGTGTAAAACTAAAGTTTATTCTACAAACAACAGAGTC1220.10922601727919781No Hit
TAGTATGGGCTGTTTGAGAATAAGAACCAACCACTTCTAATATTTGACACAC1220.10922601727919781No Hit
GACATTGGGATCGCTAAATACAATGCTGTGAGGTCTATAGTTCCTGTAACCC1210.10833072205559784No Hit
GCGATTGGGGTCTCAAATTCCTTAAATAATCTTTGGTGTTTTGGTAATAAAT1210.10833072205559784No Hit
CTTAGAGGGCACTGTAAGGCTAACTTAGCATTAACCTTTTAAGTTAAAGATT1210.10833072205559784No Hit
AGAAATGGGCTGTCTGAGGGCTTCTCGCACAGCCGCTGGCTCCGGAAGGTGA1210.10833072205559784No Hit
TGTCGCGGGATGAAATGTATTATTTTGAACAGAGTTTGTGATTTGGTAGTTA1210.10833072205559784No Hit
TTGGCTGGGAATAAGGATTGTGTGTAGGTGAATAAAGACAGCTAAGTGAATG1200.10743542683199785No Hit
TTTGTAGGGCAAATAAATAAAAATTCATTTGTACAGGCTTAAGAATGAAACT1190.10654013160839787No Hit
ATTGTGGGGTGAAATTACCATTTTTAAATACCTTTGAAATATCCTTTGTGTC1190.10654013160839787No Hit
ACGTTGGGGGCTTTCACAATAAAACTAAGGCTTTATTTTTCCCTGATTTCAC1190.10654013160839787No Hit
GCCAAGGGATGAACACTGTCATCAATGTTCAGTTCAAAAGCCTGAAAGTTTA1190.10654013160839787No Hit
GTCATCGGGAGAACGTTACCTGGTCACCAGCCTGATTCTGGTGGTTACCATG1190.10654013160839787No Hit
TCATGCCGGGAGCTCAGTTAATAAAACAATTTCAGAATTAAAGAAATTTTCT1190.10654013160839787No Hit
CACCGAGGGATCTCATCATCAAAACCTTCATTTTTTTTTTAAAAAAAAGCAT1190.10654013160839787No Hit
CTTACTGGGAAATTAACAAAGCTTATTCTCTTGGGGGCCAAGAGAATCTTGG1180.10564483638479788No Hit
TCGATAGGGGACTCAAATGATCACAATTAACAGAGATCTGTTAATGGCTTTT1180.10564483638479788No Hit
GTCTGCGGGAGATAAATACCTTTCAAGTGTGAGCTTAGACGTCAACCCTAAA1170.10474954116119789No Hit
ATGCCGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1170.10474954116119789No Hit
CATGGTGGGAGTCCAAATATAAGAAACTGTCATGTTTTGTGGGAAAATAATT1170.10474954116119789No Hit
TCATATAGGGCAAAGAAATTAGTTCATTTCTGCTTCCACTTTATGTAATTTA1170.10474954116119789No Hit
GCACATGGGTCTGGATTTGGGATCCTGGAGCCCAAATCAGTCATGTGGGTGG1160.10385424593759793No Hit
TTAGTAGGGAGAGCTAGACCTGGTGCTTGGAGAAGAGACATCTTTGGAAGAA1160.10385424593759793No Hit
GATGACGGGAGCTCTCAGGCTCCAATGTTCAGCACATTTTTTCATTTGTTTT1160.10385424593759793No Hit
TTGTGCTGGGAACGGAAGAAAAAATGAGTGCTTTTAAAGATTTTTTAAAATG1160.10385424593759793No Hit
TGCTCAGGGCTGTGGAGGAGGAGATGTCTGTGCCCTTGAAAGAACTGATTGA1160.10385424593759793No Hit
ACAGCTGGGGGGACCTCTGCCCAAGAAAGCCTGGGTGTTGTCCAAGGTTTCC1160.10385424593759793No Hit
GTGCAGGGGCATGATTCTCTCTTCGTTAGCTTCTGTCTTATAAATAAAGGAA1160.10385424593759793No Hit
AAACACGGGTGACATTTGGGGCCAGATAATTCTTTGTTGTGGGGCTGTCTGG1160.10385424593759793No Hit
ATCGCTGGGAATCAGGCTCTGTTTGCACTTTATATTTTAGCAGATACAGTCT1160.10385424593759793No Hit
AAAACTGGGGTACTACACAGGTTTGGAAAGCAGATCATTCTAACAGATATCC1160.10385424593759793No Hit
TTCTTTGGGCAAACCAGCCTTCCTGCTCTTTCTCATGCTTCATATCTCTCTC1150.10295895071399794No Hit
GTAATGGGGAATGAAATGCCTCCCATGACAGCCACAGTCCACAGTGGGGCCT1150.10295895071399794No Hit
TACTGGGGGGTTAATGTCAATTAAAATGGAATCACTAAAACTTTTATGGAAA1150.10295895071399794No Hit
TACATTGGGAAAATGGCACATTCTGCACATGTATTCCAGAACTTAAAGTATA1150.10295895071399794No Hit
TTTAAGGGGACATGTCCCATTAAAAAGTGGGAGATTTTAAATAATTTCTTTA1150.10295895071399794No Hit
TCGTCAGGGTGAATAACCAACCCAGTGATTTACATGCTGATAGCTAAGTGAG1140.10206365549039796No Hit
TTGGCTGGGGTTACCAGAAACATGATCATAGAGCAAATTCGGATATGAATGG1140.10206365549039796No Hit
GCTCTAGGGAAGAAGATGAAGATCCCAGAATGTGGAAACAAAAAGCTGACAA1140.10206365549039796No Hit
GCTTTAGGGCAGTAATTTATTCTTTTAGCAAACCTGATACTGATAACTGTTT1140.10206365549039796No Hit
TTTCTGGGGGCTTCCCAACATGGCGCAGTCTATTAACATCACGGAGCTGAAT1140.10206365549039796No Hit
CTAAATGGGTAAATGAGTTAACATAGTAAAGCTTTAGAATAGTGTGTAGCAA1130.10116836026679797No Hit
GAAGTTGGGATAGGATGAGCTATTCTACGTTTAACACTTAACACATGCCTGG1130.10116836026679797No Hit
ATGTCAGGGAGGTGGCCTCTGGACGTCACCGTTGCCCTGCATGGTGGCCCCA1130.10116836026679797No Hit
TTTTAAGGGATGTGTAGAAATGGACATGAAAAATCTGAAATGAAAAAACAAA1130.10116836026679797No Hit
ATGGATGGGAGTCTAGAATACGACTGCCTTTCATTTTGCATTCACCTTCTTC1130.10116836026679797No Hit
TAAACAGGGAGTCCCACAGTCTAGGGAGATCTGAGGGCATCCGACAAGGCCC1120.10027306504319801No Hit
TTTCATGGGGGATTGTGATGTGCTTTCTTGCTGTGGTTTTGATGTGCTTTTC1120.10027306504319801No Hit
AGCTTAGGGAAGCCTGCTATGTTTACTGTTTTATGAATGTCAACATTCTCCA1120.10027306504319801No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)