FastQCFastQC Report
Sat 31 Dec 2022
SRR5846403.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846403.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111936
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC21851.9520082904516864No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT20071.7929888507718694No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT3750.33501286449399653No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA2980.26622355631789596No Hit
ATTCACGGGCAGCACTGAAATAGGTTGAGGGTTCCCTTCCCATTTACCTTGA2130.19028730703259006No Hit
GCGTTTGGGTCCGATCTTCATGTGGGCGATCTCAAACCGGGTGTTCTTACAG2030.18135363064608348No Hit
CACTCTGGGAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCT2020.18046026300743281No Hit
CGAACCGGGGATCCTGAGAGATGAGAGGCAGCCTAAATAGACCTCTTTCCTG1930.1724199542595769No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC1860.1661663807890223No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1840.164379645511721No Hit
TACTCCGGGTCTGCACATGTACTCCAGAACTTAAAGTATAATTAAAAAACAG1820.16259291023441966No Hit
TTGTTAGGGGGGCTGGGCGCCGAGTGGGACAGCGCTGGTGCGGAGACTGCTT1810.16169954259576902No Hit
ATGAAAGGGAGATTCTAGCTCTGACACTTACTAGTGATTTTGAGTATGTTGT1780.15901943967981702No Hit
ATTCGAGGGCAAAGCCATTTATGTTTCTGGCTCAGCAAGCCTTTCAGTTCTT1770.15812607204116638No Hit
ATTAATGGGCGTAAAATTAATCATTTGTGACTTAACTTTGAATTTTTATAAT1760.15723270440251574No Hit
CGGCTGGGATGGATAATATGTCCAAAAATTTATAAAAATCAAATGGAAATTT1730.15455260148656375No Hit
CCACAAGGGATTTTGACCCCACTTACAGATCAGAAAACTGAGGCAGATATCT1720.15365923384791308No Hit
ATGTTTGGGCTGGGAAGGAGAACAACAATTATATTCCCCCATTTCAAGAAGG1700.15187249857061177No Hit
TTTAACGGGATGTCTGCATTACTCATTGCTAAAAATGTGTAGCACAGAACTG1690.15097913093196114No Hit
GTCAGCGGGGCCTGTAAGTGTACATGTAGTGGTGTGGTGTTCTGTCTAGAGG1640.14651229273870783No Hit
ACACTGGGACCACTACTCTATGCACTACTCGGCGCTGCCCGGTTCGCGGCCC1640.14651229273870783No Hit
TTATCTGGGATGGCGTTAGCATCCATATGATCCATAGGCTGGTTGCTGGGAA1630.14561892510005717No Hit
ACGCACGGGAATAAAGGAAAGGATCCAACAGAAAATTCATTAAGGAATCTTT1630.14561892510005717No Hit
TTGCTGGGGCAGCCATTTACTTGCTATTTTAATCAACATCAAAGACCAGATG1610.14383218982275586No Hit
TGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAG1610.14383218982275586No Hit
CTTTGTGGAGACTGCACATCCGGACCTGCCCATGTCTCAAAACAAACACATG1600.1429388221841052No Hit
TCAAAGGGCGATCTATTAAAGGGGAGATCACTAACTTTACACACACACACAC1570.14025871926815323No Hit
CAATCGGGGATATTTTAAGAGTTTTCTTTTTTCTATGATGCTTTTATTTGTG1550.13847198399085192No Hit
CTTTAGGGGAATGCCTTATTTTTGAATTCTTTAGATTTTTAAATTGGAGAAA1540.13757861635220126No Hit
AGGCTGGGGGCCTTTTCAGGCGTGGCCTCCTTTCCATCTAGCACAGCATCTT1520.13579188107489995No Hit
GCTGTAGGGGTGGTCTCTTGAATTTTGTAAGTTCATCTGAGGAGAAAAGATT1510.13489851343624928No Hit
TGTACGGGGCATTTAATTTTCTCATAGATTTAAGTAAACAGATGTATTTTGC1510.13489851343624928No Hit
GATGAAGGGAACCTCTGTTGCTTAGAAAGCTTCCAGTGTTCTTAGATCTCTC1510.13489851343624928No Hit
TCCATTGGGAAGACTTTAAAACCTAAATGACTTTTGACATACAAACTCTTCT1510.13489851343624928No Hit
AACAGAGGGTTAACAACCGATGCAACATTAAAAATGTGTTAGTGTGCTGTGC1500.13400514579759862No Hit
AGCTTCGGGGACCTTTGAAATTTGCATGTGACCTCATCTAGCTATGAATTCT1500.13400514579759862No Hit
TACGCAGGGATTCTTACTCTGTGATTAAGGTTTTTATGAGTAACCAAGGAAC1500.13400514579759862No Hit
GAAAAAGGGACTAGGTGATCCAGTAAGTTATTTGATAAGGGGCATTTTCTTC1490.13311177815894798No Hit
TTTGTCGGGGATCTGAGAAGAGCCTCATTCAATAGTTTTAAATGTTAAAAGG1490.13311177815894798No Hit
TTGTTAGGGCGATCTTGGAGAGGGATGACCCCAATACGCAAAACTAACCCCC1480.1322184105202973No Hit
TTCTTAGGGACTATTAATTTTTGTTTGTGGAGGAGGGGAAAAGTGCTTTAAA1480.1322184105202973No Hit
TTTCATGGGGGATTGTGATGTGCTTTCTTGCTGTGGTTTTGATGTGCTTTTC1460.13043167524299598No Hit
CAAGATGGGCTTGTGCCTTTCATGTAAGTTAAATTTGCTCATACAGCTTGAA1460.13043167524299598No Hit
ATCATCGGGATAGGAGGTAATCACCAGACAACTGCGGAATGTGGAGCACTGA1460.13043167524299598No Hit
GTCAGCGGGTGCCCTGAATGACCATTGAGTCAATGATGAAATTAATATGGAA1450.12953830760434534No Hit
TTGGACGGGATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATTTCT1450.12953830760434534No Hit
ACGTTGGGGGCTTTCACAATAAAACTAAGGCTTTATTTTTCCCTGATTTCAC1450.12953830760434534No Hit
TTCGATGGGCAGTCCACTCTGTCTTTAAAACATAGTGATTACAATATTTAGA1420.12685820468839337No Hit
AAAAGCGGGATAAGTGAAATGATGAGTTATTTGTCTTTATGTGCCTGCCTTA1420.12685820468839337No Hit
TTGCAAGGGATTGAACGCAACAGGAGGGTATTTCAGCAAATTCACGACAAGG1410.1259648370497427No Hit
GATTTTGGGAGGCTACAGTCTGTGCCATGTTGATGTACAGTTTCTGAAATTG1410.1259648370497427No Hit
TCGACTGGGTGAGTGCATCTTGTATGTGCCTAATATTGATAGGATGAGATGT1400.12507146941109204No Hit
TACAACGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1400.12507146941109204No Hit
ACACGGGTGGGGAGAAAAAGCCTTTAGTGAATTTTCTTGAGGGTGATAAATC1390.12417810177244139No Hit
TCAGCAGGGATATAGCAAAAAGTAATCATATCTGGCTAGATAGCCATTTCTC1390.12417810177244139No Hit
TCAGCGGGGCAATGAGTCATGTCTGCATGATTTCATTATGTAGTATTTCATT1380.12328473413379074No Hit
GATGACGGGAGCTCTCAGGCTCCAATGTTCAGCACATTTTTTCATTTGTTTT1380.12328473413379074No Hit
TATGGCGGGACTTCCTAGCCCTTTTATCAACTCCTAGAATAGTTAAAGAGAG1380.12328473413379074No Hit
AAGTATGGGAACCATTTTATTAATATATAATGTCTTTGTCTCTTGTAACAAT1370.12239136649514008No Hit
AGAAATGGGCTGTCTGAGGGCTTCTCGCACAGCCGCTGGCTCCGGAAGGTGA1370.12239136649514008No Hit
TCGGTTGGGCTGGGAATCAGGAGCCTGGACACTGCCATCTCTAGAGCAGAGT1360.12149799885648942No Hit
CACTTTGGGACTGTAAGTCTCAATTAACAGCAGAATCTCAGAGAAAAGCTGT1360.12149799885648942No Hit
ATGAGTGGGCAGTCTTTACTAATTTTAAATATTATTCTTTTTTTATATTTCT1330.11881789594053743No Hit
CTTAAGGGTGCCGGTGTTTTAAGAAAGCCAGATGCCTGGATTGGACTCTGGG1330.11881789594053743No Hit
CGCGACGGGGGGGTCAGCTGCCCCACTCCTGACTTTTTTATTTTTAATGGTC1330.11881789594053743No Hit
AGTAGAGGGGGCCAAGTGTAAAACTAAAGTTTATTCTACAAACAACAGAGTC1330.11881789594053743No Hit
TTAGTGAGGGCGATCTGAAAAAGGGAGACTCCAGAGTGGCAGTATAGTGTCT1320.1179245283018868No Hit
TTAGTGGGGTGATGGTTACAATGTGCAGCCAGACACCGTGGCCCCAATCTGG1320.1179245283018868No Hit
GCAACTGGGTGATGAAAGTGGTGGACTTGAAATTTCCCGTTCATCTCCCAAA1320.1179245283018868No Hit
TGTTAAGGGGGAACTGGAAGTCGCAGTTTAGGCCTGAAAATACTAGAACCTT1310.11703116066323614No Hit
AGCTCTGGGTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTA1300.11613779302458549No Hit
CATTTTGGGACTTCATTTTCTAGTTGAAAAAAGTGTTGTTTTGCCTTTGCTC1300.11613779302458549No Hit
ATGCCGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1300.11613779302458549No Hit
TTGGCTGGGGTTACCAGAAACATGATCATAGAGCAAATTCGGATATGAATGG1290.11524442538593481No Hit
CTTACTGGGAAATTAACAAAGCTTATTCTCTTGGGGGCCAAGAGAATCTTGG1290.11524442538593481No Hit
GACAATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG1290.11524442538593481No Hit
GTAGGCGGGATATAATCTCCTGGTGTGCCATTTTTTAAGCCCGTTGGAAAAG1290.11524442538593481No Hit
TGTCGCGGGATGAAATGTATTATTTTGAACAGAGTTTGTGATTTGGTAGTTA1290.11524442538593481No Hit
ACGACGGGATATATAACGTGATATATATATATACACAAAGACACACACAGTG1290.11524442538593481No Hit
TGTCCAGGGCACCCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCC1280.11435105774728416No Hit
AAATCAGGGATCTTTCCAAATTTGCCATAACAGATTTAGGTTTCTTTCCTTT1280.11435105774728416No Hit
TTGGTGGGAGATCCAGTCACAGGTTGTGGCTCAGATACAGGCTCAGCAAAGT1280.11435105774728416No Hit
ACAGCTGGGGGGACCTCTGCCCAAGAAAGCCTGGGTGTTGTCCAAGGTTTCC1280.11435105774728416No Hit
AACACTGGGATATTTTTATAAGTAAAAAAATCTTTCTAAACAACAAATACCT1270.1134576901086335No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1270.1134576901086335No Hit
CAACAAGGGCTATCACAGTATCTATAACATAGTAAGAGCTTTATTCGGGTCT1270.1134576901086335No Hit
CCGCGAAGGGAAGATATGAGAATATTATTGATGAATTTTCATAAGGTGGAAT1260.11256432246998285No Hit
AACATGGGGACCTCAACTGTGACTTGTAAGGTCTGCTTTGTATATAAGATAT1260.11256432246998285No Hit
GCCTAGGGCACATATTTTTCAAACCTATACGAAATGCCTCTTCTCATGTTTT1260.11256432246998285No Hit
CATGGTGGGAGTCCAAATATAAGAAACTGTCATGTTTTGTGGGAAAATAATT1260.11256432246998285No Hit
TTGGCTGGGAATAAGGATTGTGTGTAGGTGAATAAAGACAGCTAAGTGAATG1260.11256432246998285No Hit
GCACATGGGTCTGGATTTGGGATCCTGGAGCCCAAATCAGTCATGTGGGTGG1250.11167095483133219No Hit
GAAACTGGGAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGG1250.11167095483133219No Hit
ATCCAGGAGAAAATAAAGTAAATTATTTTAAAAAATTACCTATATTCCCAAC1250.11167095483133219No Hit
GACATTGGGATCGCTAAATACAATGCTGTGAGGTCTATAGTTCCTGTAACCC1240.11077758719268153No Hit
TTACGCGGGCTGGTACTGAATGCCGCTATCACCATGGGGATGGTTCTTCTAA1240.11077758719268153No Hit
CTCAGAGGAGATCTGTAGGGACTTGTACAACATTGTGGATGTGTAAACAGGC1230.10988421955403088No Hit
TGATGGGGAGACGAATTTGGAGACTGGGATTAGAGCCTTTGGAGGTCAAAGC1220.10899085191538022No Hit
AGAAATGGGATGTGCATTTTAAATGCTAGAACTTAAAAACCCACTGAAACAC1220.10899085191538022No Hit
ACATTTGGGAAGAAGAAATGATCATTTTGGAACCAGACAAGAATCCAAAGAA1220.10899085191538022No Hit
GTACGAGGGATAACTATAAATTGCATACCCTAACGTAATTATGCATTAATTA1220.10899085191538022No Hit
GCACCTCGGGCGAGGCAAAGATGTTAATTCTTCCCAAATTGATATATAGGTT1210.10809748427672956No Hit
ATATCAGGGACCCTGGGCCCCTGGGCCCTGCTTCTCTGCTCCCCTGGGTGAT1210.10809748427672956No Hit
AACGCTGGGACCTCCTACTGTTGATTCAACTCAACAGCCTAACCCTTTGCCG1200.10720411663807891No Hit
TTATCAGGGAGAGACGCCAGAGGTGCAGCTCCAGCAGCAATGGCAGTGACGG1200.10720411663807891No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1200.10720411663807891No Hit
TTAGTAGGGAGAGCTAGACCTGGTGCTTGGAGAAGAGACATCTTTGGAAGAA1200.10720411663807891No Hit
GTAATGGGGAATGAAATGCCTCCCATGACAGCCACAGTCCACAGTGGGGCCT1190.10631074899942826No Hit
CTAAATGGGTAAATGAGTTAACATAGTAAAGCTTTAGAATAGTGTGTAGCAA1190.10631074899942826No Hit
TTTTAAGGGATGTGTAGAAATGGACATGAAAAATCTGAAATGAAAAAACAAA1190.10631074899942826No Hit
GCGATTGGGGTCTCAAATTCCTTAAATAATCTTTGGTGTTTTGGTAATAAAT1190.10631074899942826No Hit
TACATTGGGAAAATGGCACATTCTGCACATGTATTCCAGAACTTAAAGTATA1190.10631074899942826No Hit
GCCAAGGGATGAACACTGTCATCAATGTTCAGTTCAAAAGCCTGAAAGTTTA1190.10631074899942826No Hit
ATGGATGGGAGTCTAGAATACGACTGCCTTTCATTTTGCATTCACCTTCTTC1190.10631074899942826No Hit
ATTGGCGGGGTGTAACCAAGGCCTGTTTGAATGCCTGGCCCTAAACCTGCAC1180.10541738136077758No Hit
GTGCAGGGGCATGATTCTCTCTTCGTTAGCTTCTGTCTTATAAATAAAGGAA1180.10541738136077758No Hit
TCATGCCGGGAGCTCAGTTAATAAAACAATTTCAGAATTAAAGAAATTTTCT1180.10541738136077758No Hit
TCGTCAGGGTGAATAACCAACCCAGTGATTTACATGCTGATAGCTAAGTGAG1170.10452401372212693No Hit
GCGTTGGGGTCCAGTAGACTAAGATTGAGTGTTCTTTTTGTTCAGCAACTCT1170.10452401372212693No Hit
GTCTTTGGGATAGCCATTGATGTATGCATCTCTTGGCTGTACTATAAGAACA1170.10452401372212693No Hit
GTTCATGGGTTTCTTAACCTGTCTAGCTGTCGGAAAAGGTAACAGAAGATGG1170.10452401372212693No Hit
TGTTCTGGGAGCTGAGAACTGCCCGATACACGGCATCATCCCATCTCTAATT1160.10363064608347627No Hit
GTAACCGGGGCGAGCGTTGGTGCTGCTTGATAACCCATGCACGGACGAAACC1160.10363064608347627No Hit
GCAACGAGAGAGAAAATATTCGTATTTGTTTATGGTTGTATACCTATTTTGA1160.10363064608347627No Hit
TGAAAAGGGGAATTCAACTATTTTATGTCATTCTCTTTCTCACGCTTTTATG1150.10273727844482561No Hit
GCTTTAGGGCAGTAATTTATTCTTTTAGCAAACCTGATACTGATAACTGTTT1150.10273727844482561No Hit
ATTGTGGGGTGAAATTACCATTTTTAAATACCTTTGAAATATCCTTTGTGTC1150.10273727844482561No Hit
CGATAGGGGTACTACAGTGATATAAAAACCAGCAGTTTTTACACTAAATTTT1140.10184391080617496No Hit
GTACATGGGACCTGCAGGAACAACATGGAGAGCTTTCTTTGGAATTCCTTAG1130.1009505431675243No Hit
TTATCCGGGGATCTTCACGTGGGAATGCCCCAACTAAATACTACCGTATGGC1130.1009505431675243No Hit
CTTACGGGTATGCATCTGCAAGTCAGGAAGGCCAAAGATGACCACAAACCAC1130.1009505431675243No Hit
TAGTTTGGGGAGGCCAAGTGTGCGTCCAGGGGAACAGCACAGGTTAATGCGT1130.1009505431675243No Hit
AAGCCCGGGATCACCCAACTAAAAATATTAAACACAAACTACCACCTACCTC1130.1009505431675243No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1120.10005717552887365No Hit
GAGATATGGCCTCGTGACAAGGGAAAAACCTGACCATCCCCCAGCCCAACAC1120.10005717552887365No Hit
TTTACTGGGTCCCAATTATAAATGAAACTGGCTTTCTTCAAAAGACCAGCTG1120.10005717552887365No Hit
TAGCTAGGGCGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTA1120.10005717552887365No Hit
CACCGAGGGATCTCATCATCAAAACCTTCATTTTTTTTTTAAAAAAAAGCAT1120.10005717552887365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)