FastQCFastQC Report
Sat 31 Dec 2022
SRR5846402.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846402.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112377
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT21451.9087535705704903No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC21161.8829475782410992No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT3570.31768066419285085No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3140.2794166066009949No Hit
ATTCACGGGCAGCACTGAAATAGGTTGAGGGTTCCCTTCCCATTTACCTTGA2560.22780462194221238No Hit
ATGTTTGGGCTGGGAAGGAGAACAACAATTATATTCCCCCATTTCAAGAAGG2090.18598111713250934No Hit
GCGTTTGGGTCCGATCTTCATGTGGGCGATCTCAAACCGGGTGTTCTTACAG2020.17975208450127694No Hit
CACTCTGGGAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCT1940.1726331900655828No Hit
TACTCCGGGTCTGCACATGTACTCCAGAACTTAAAGTATAATTAAAAAACAG1890.16818388104327398No Hit
TTATCTGGGATGGCGTTAGCATCCATATGATCCATAGGCTGGTTGCTGGGAA1850.1646244338254269No Hit
ATGAAAGGGAGATTCTAGCTCTGACACTTACTAGTGATTTTGAGTATGTTGT1840.16373457202096514No Hit
TTTGTCGGGGATCTGAGAAGAGCCTCATTCAATAGTTTTAAATGTTAAAAGG1750.15572581578080924No Hit
CAATCGGGGATATTTTAAGAGTTTTCTTTTTTCTATGATGCTTTTATTTGTG1720.15305623036742394No Hit
CATTTTGGGACTTCATTTTCTAGTTGAAAAAAGTGTTGTTTTGCCTTTGCTC1710.15216636856296217No Hit
AGCTTCGGGGACCTTTGAAATTTGCATGTGACCTCATCTAGCTATGAATTCT1710.15216636856296217No Hit
CGAACCGGGGATCCTGAGAGATGAGAGGCAGCCTAAATAGACCTCTTTCCTG1700.1512765067585004No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1690.15038664495403864No Hit
CCACAAGGGATTTTGACCCCACTTACAGATCAGAAAACTGAGGCAGATATCT1690.15038664495403864No Hit
TTTAACGGGATGTCTGCATTACTCATTGCTAAAAATGTGTAGCACAGAACTG1680.14949678314957687No Hit
TGTCCAGGGCACCCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCC1670.1486069213451151No Hit
CTTTGTGGAGACTGCACATCCGGACCTGCCCATGTCTCAAAACAAACACATG1650.14682719773619157No Hit
GCGTTGGGGTCCAGTAGACTAAGATTGAGTGTTCTTTTTGTTCAGCAACTCT1640.1459373359317298No Hit
CGGCTGGGATGGATAATATGTCCAAAAATTTATAAAAATCAAATGGAAATTT1640.1459373359317298No Hit
TCAAAGGGCGATCTATTAAAGGGGAGATCACTAACTTTACACACACACACAC1630.14504747412726804No Hit
GCCAAGGGATGAACACTGTCATCAATGTTCAGTTCAAAAGCCTGAAAGTTTA1620.14415761232280627No Hit
GTCAGCGGGGCCTGTAAGTGTACATGTAGTGGTGTGGTGTTCTGTCTAGAGG1610.1432677505183445No Hit
AAATCAGGGATCTTTCCAAATTTGCCATAACAGATTTAGGTTTCTTTCCTTT1610.1432677505183445No Hit
TTCGATGGGCAGTCCACTCTGTCTTTAAAACATAGTGATTACAATATTTAGA1600.14237788871388274No Hit
ATTCGAGGGCAAAGCCATTTATGTTTCTGGCTCAGCAAGCCTTTCAGTTCTT1600.14237788871388274No Hit
TTACGCGGGCTGGTACTGAATGCCGCTATCACCATGGGGATGGTTCTTCTAA1590.14148802690942097No Hit
CTTAAGGGTGCCGGTGTTTTAAGAAAGCCAGATGCCTGGATTGGACTCTGGG1580.1405981651049592No Hit
TTAGTGGGGTGATGGTTACAATGTGCAGCCAGACACCGTGGCCCCAATCTGG1560.13881844149603567No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC1550.1379285796915739No Hit
GCTGTAGGGGTGGTCTCTTGAATTTTGTAAGTTCATCTGAGGAGAAAAGATT1540.13703871788711214No Hit
TTGTTAGGGGGGCTGGGCGCCGAGTGGGACAGCGCTGGTGCGGAGACTGCTT1520.1352589942781886No Hit
ATTAATGGGCGTAAAATTAATCATTTGTGACTTAACTTTGAATTTTTATAAT1520.1352589942781886No Hit
CAAGATGGGCTTGTGCCTTTCATGTAAGTTAAATTTGCTCATACAGCTTGAA1510.13436913247372684No Hit
TTGCAAGGGATTGAACGCAACAGGAGGGTATTTCAGCAAATTCACGACAAGG1500.13347927066926507No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1490.1325894088648033No Hit
ACGCACGGGAATAAAGGAAAGGATCCAACAGAAAATTCATTAAGGAATCTTT1490.1325894088648033No Hit
GCAACGAGAGAGAAAATATTCGTATTTGTTTATGGTTGTATACCTATTTTGA1480.13169954706034154No Hit
TGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAG1480.13169954706034154No Hit
ATCATCGGGATAGGAGGTAATCACCAGACAACTGCGGAATGTGGAGCACTGA1480.13169954706034154No Hit
AGGCTGGGGGCCTTTTCAGGCGTGGCCTCCTTTCCATCTAGCACAGCATCTT1480.13169954706034154No Hit
CACTTTGGGACTGTAAGTCTCAATTAACAGCAGAATCTCAGAGAAAAGCTGT1480.13169954706034154No Hit
GAAAAAGGGACTAGGTGATCCAGTAAGTTATTTGATAAGGGGCATTTTCTTC1460.12991982345141798No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1460.12991982345141798No Hit
TCCATTGGGAAGACTTTAAAACCTAAATGACTTTTGACATACAAACTCTTCT1460.12991982345141798No Hit
CTTTAGGGGAATGCCTTATTTTTGAATTCTTTAGATTTTTAAATTGGAGAAA1450.1290299616469562No Hit
GTGCAGGGGCATGATTCTCTCTTCGTTAGCTTCTGTCTTATAAATAAAGGAA1450.1290299616469562No Hit
TTGCTGGGGCAGCCATTTACTTGCTATTTTAATCAACATCAAAGACCAGATG1430.12725023803803268No Hit
TTCTTAGGGACTATTAATTTTTGTTTGTGGAGGAGGGGAAAAGTGCTTTAAA1420.1263603762335709No Hit
TCATGCCGGGAGCTCAGTTAATAAAACAATTTCAGAATTAAAGAAATTTTCT1420.1263603762335709No Hit
TTGTTAGGGCGATCTTGGAGAGGGATGACCCCAATACGCAAAACTAACCCCC1410.12547051442910914No Hit
GTAACCGGGGCGAGCGTTGGTGCTGCTTGATAACCCATGCACGGACGAAACC1410.12547051442910914No Hit
GACAATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG1410.12547051442910914No Hit
TAGTTTGGGGAGGCCAAGTGTGCGTCCAGGGGAACAGCACAGGTTAATGCGT1410.12547051442910914No Hit
ATCCAGGAGAAAATAAAGTAAATTATTTTAAAAAATTACCTATATTCCCAAC1410.12547051442910914No Hit
TGTTCTGGGAGCTGAGAACTGCCCGATACACGGCATCATCCCATCTCTAATT1380.12280092901572387No Hit
AACAGAGGGTTAACAACCGATGCAACATTAAAAATGTGTTAGTGTGCTGTGC1380.12280092901572387No Hit
CTTCAGGGAGACTACTGACTTGGACCAGCAGGGGAATGGCTGTGACAATAAA1380.12280092901572387No Hit
AACACTGGGATATTTTTATAAGTAAAAAAATCTTTCTAAACAACAAATACCT1370.1219110672112621No Hit
TTATCCGGGGATCTTCACGTGGGAATGCCCCAACTAAATACTACCGTATGGC1370.1219110672112621No Hit
TTGGACGGGATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATTTCT1360.12102120540680034No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1360.12102120540680034No Hit
TGTCGCGGGATGAAATGTATTATTTTGAACAGAGTTTGTGATTTGGTAGTTA1360.12102120540680034No Hit
TGTACGGGGCATTTAATTTTCTCATAGATTTAAGTAAACAGATGTATTTTGC1350.12013134360233857No Hit
AGAAATGGGATGTGCATTTTAAATGCTAGAACTTAAAAACCCACTGAAACAC1350.12013134360233857No Hit
CATGGTGGGAGTCCAAATATAAGAAACTGTCATGTTTTGTGGGAAAATAATT1350.12013134360233857No Hit
GCCTAGGGCACATATTTTTCAAACCTATACGAAATGCCTCTTCTCATGTTTT1340.1192414817978768No Hit
TCAGCAGGGATATAGCAAAAAGTAATCATATCTGGCTAGATAGCCATTTCTC1340.1192414817978768No Hit
ACAGCTGGGGGGACCTCTGCCCAAGAAAGCCTGGGTGTTGTCCAAGGTTTCC1340.1192414817978768No Hit
TCGACTGGGTGAGTGCATCTTGTATGTGCCTAATATTGATAGGATGAGATGT1330.11835161999341504No Hit
GATTTTGGGAGGCTACAGTCTGTGCCATGTTGATGTACAGTTTCTGAAATTG1330.11835161999341504No Hit
GTACGAGGGATAACTATAAATTGCATACCCTAACGTAATTATGCATTAATTA1330.11835161999341504No Hit
TCAGCGGGGCAATGAGTCATGTCTGCATGATTTCATTATGTAGTATTTCATT1320.11746175818895327No Hit
CGCGACGGGGGGGTCAGCTGCCCCACTCCTGACTTTTTTATTTTTAATGGTC1320.11746175818895327No Hit
ATGAGTGGGCAGTCTTTACTAATTTTAAATATTATTCTTTTTTTATATTTCT1300.11568203458002972No Hit
AGCTCTGGGTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTA1300.11568203458002972No Hit
ACATTTGGGAAGAAGAAATGATCATTTTGGAACCAGACAAGAATCCAAAGAA1300.11568203458002972No Hit
AACGCTGGGACCTCCTACTGTTGATTCAACTCAACAGCCTAACCCTTTGCCG1290.11479217277556795No Hit
TTGTGCTGGGAACGGAAGAAAAAATGAGTGCTTTTAAAGATTTTTTAAAATG1290.11479217277556795No Hit
TAGTATGGGCTGTTTGAGAATAAGAACCAACCACTTCTAATATTTGACACAC1280.11390231097110619No Hit
TTAGTAGGGAGAGCTAGACCTGGTGCTTGGAGAAGAGACATCTTTGGAAGAA1270.11301244916664442No Hit
ACACGGGTGGGGAGAAAAAGCCTTTAGTGAATTTTCTTGAGGGTGATAAATC1270.11301244916664442No Hit
ACGACGGGATATATAACGTGATATATATATATACACAAAGACACACACAGTG1270.11301244916664442No Hit
ATTTAAGGGATGCTGGGTAATATCACTGAAGCTCTTCCTGAGATTTCTTGAG1260.11212258736218265No Hit
TTTCTGGGGGCTTCCCAACATGGCGCAGTCTATTAACATCACGGAGCTGAAT1260.11212258736218265No Hit
ATGATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1250.11123272555772089No Hit
TACAACGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1250.11123272555772089No Hit
GACATTGGGATCGCTAAATACAATGCTGTGAGGTCTATAGTTCCTGTAACCC1240.11034286375325912No Hit
GTAATGGGGAATGAAATGCCTCCCATGACAGCCACAGTCCACAGTGGGGCCT1240.11034286375325912No Hit
GAAGTTGGGATAGGATGAGCTATTCTACGTTTAACACTTAACACATGCCTGG1240.11034286375325912No Hit
TTTCATGGGGGATTGTGATGTGCTTTCTTGCTGTGGTTTTGATGTGCTTTTC1240.11034286375325912No Hit
TTCTTTGGGCAAACCAGCCTTCCTGCTCTTTCTCATGCTTCATATCTCTCTC1230.10945300194879735No Hit
GTCAGCGGGTGCCCTGAATGACCATTGAGTCAATGATGAAATTAATATGGAA1230.10945300194879735No Hit
ATCGTTGGGCCGATCTGTGTTAGGGAACAGACAATTTTTAAAAGAACCTAAG1230.10945300194879735No Hit
AAAAGCGGGATAAGTGAAATGATGAGTTATTTGTCTTTATGTGCCTGCCTTA1230.10945300194879735No Hit
GCAACTGGGTGATGAAAGTGGTGGACTTGAAATTTCCCGTTCATCTCCCAAA1230.10945300194879735No Hit
CCGCGAAGGGAAGATATGAGAATATTATTGATGAATTTTCATAAGGTGGAAT1220.10856314014433559No Hit
TTGGTGGGAGATCCAGTCACAGGTTGTGGCTCAGATACAGGCTCAGCAAAGT1220.10856314014433559No Hit
GGCAATGGGGATTAAACTAATGGGAGGAAATAAATCAACAAAGTCTCCATTA1220.10856314014433559No Hit
ACGTTGGGGGCTTTCACAATAAAACTAAGGCTTTATTTTTCCCTGATTTCAC1220.10856314014433559No Hit
ATAGCCGGGATATTAAAAATAAAAAGACAAGAAAATTGAAGATCATTTTTCA1220.10856314014433559No Hit
TAGCTAGGGCGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTA1220.10856314014433559No Hit
CAGCCTGGGCGACAGAGCAAGACTCCATCTCAAAAACAAACAAACAAAAAAG1210.10767327833987382No Hit
GGTACTGGGGTTAAAGTATCTTTGATTGCAAGTGTTAGAAACCCAACTCATG1210.10767327833987382No Hit
TGATGGGGAGACGAATTTGGAGACTGGGATTAGAGCCTTTGGAGGTCAAAGC1210.10767327833987382No Hit
GCACATGGGTCTGGATTTGGGATCCTGGAGCCCAAATCAGTCATGTGGGTGG1210.10767327833987382No Hit
ATGCCGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1210.10767327833987382No Hit
CGCACTGGGAGGAAGGGATGCACTGTAAAACAAAAGTATTCTTGTAACTCAG1200.10678341653541205No Hit
AGGAACGGGGGACAAAATGGGCCTAAGCTAGTTCCCTCCAGTCCTCTCTTAA1200.10678341653541205No Hit
TAAACAGGGAGTCCCACAGTCTAGGGAGATCTGAGGGCATCCGACAAGGCCC1190.10589355473095029No Hit
CTGTCAGTGGGTGGGGGAATAATGCAGCACTTGTTTAAATGTTCTGAACACT1190.10589355473095029No Hit
TGTTAAGGGGGAACTGGAAGTCGCAGTTTAGGCCTGAAAATACTAGAACCTT1190.10589355473095029No Hit
CGTGATGGGATATCTGAAGACACAGGTTAATACATGTGGATCACTTTCAGTG1190.10589355473095029No Hit
TCGGTTGGGCTGGGAATCAGGAGCCTGGACACTGCCATCTCTAGAGCAGAGT1190.10589355473095029No Hit
AGAAATGGGCTGTCTGAGGGCTTCTCGCACAGCCGCTGGCTCCGGAAGGTGA1190.10589355473095029No Hit
CGCTTTGGGAGTGATTTTCATGCAAAATTGTCTGCCTGTTTTAAAACATGGT1190.10589355473095029No Hit
TTTTAGGGGATCTAGCCTCTGGGATGAGACAGATGATCTTTCTCCGTTTTCA1180.10500369292648852No Hit
GTCTTTGGGATAGCCATTGATGTATGCATCTCTTGGCTGTACTATAAGAACA1180.10500369292648852No Hit
ACACTGGGACCACTACTCTATGCACTACTCGGCGCTGCCCGGTTCGCGGCCC1180.10500369292648852No Hit
AAGTATGGGAACCATTTTATTAATATATAATGTCTTTGTCTCTTGTAACAAT1180.10500369292648852No Hit
TTTTAAGGGATGTGTAGAAATGGACATGAAAAATCTGAAATGAAAAAACAAA1180.10500369292648852No Hit
GCGATTGGGGTCTCAAATTCCTTAAATAATCTTTGGTGTTTTGGTAATAAAT1180.10500369292648852No Hit
GGCATTGGGGGGCACCACTTTAGGCCTATAAACCTGTTTCCTTGTCCTCACA1180.10500369292648852No Hit
TAAATAAGGGGTCTGCAGTCTGGAAGCTTGTCTGCTCTATAGAAATGAAAAT1180.10500369292648852No Hit
CACCGAGGGATCTCATCATCAAAACCTTCATTTTTTTTTTAAAAAAAAGCAT1180.10500369292648852No Hit
ATTGCTGGGAGTAACAACAAATTAACTCAAAGCTAGTTCTGAAAGTAACATC1170.10411383112202675No Hit
TTTAAGGGGACATGTCCCATTAAAAAGTGGGAGATTTTAAATAATTTCTTTA1170.10411383112202675No Hit
GATGAAGGGAACCTCTGTTGCTTAGAAAGCTTCCAGTGTTCTTAGATCTCTC1170.10411383112202675No Hit
TTGGCTGGGAATAAGGATTGTGTGTAGGTGAATAAAGACAGCTAAGTGAATG1170.10411383112202675No Hit
CAACAAGGGCTATCACAGTATCTATAACATAGTAAGAGCTTTATTCGGGTCT1170.10411383112202675No Hit
TCGATAGGGGACTCAAATGATCACAATTAACAGAGATCTGTTAATGGCTTTT1170.10411383112202675No Hit
CTCAGAGGAGATCTGTAGGGACTTGTACAACATTGTGGATGTGTAAACAGGC1160.10322396931756499No Hit
TCAGCCAGGGAAAACAAAGAGGTTTGCTATATTTAAATTCTTTTTGGAATTT1160.10322396931756499No Hit
TTAGTGAGGGCGATCTGAAAAAGGGAGACTCCAGAGTGGCAGTATAGTGTCT1160.10322396931756499No Hit
GCGGCGGGATCATTCAGCTGCAATGAGGGCAATTTGCAAGAGAGAATGCTCT1160.10322396931756499No Hit
TTATCAGGGAGAGACGCCAGAGGTGCAGCTCCAGCAGCAATGGCAGTGACGG1160.10322396931756499No Hit
CTAAATGGGTAAATGAGTTAACATAGTAAAGCTTTAGAATAGTGTGTAGCAA1160.10322396931756499No Hit
GAGATATGGCCTCGTGACAAGGGAAAAACCTGACCATCCCCCAGCCCAACAC1160.10322396931756499No Hit
TAAGATGGGACTAGAACCAATGTTTGAAGTCCCTAATTCTGATATCGTATGT1160.10322396931756499No Hit
TACGCAGGGATTCTTACTCTGTGATTAAGGTTTTTATGAGTAACCAAGGAAC1160.10322396931756499No Hit
ATGATAGGGGTGAAGTAAAAGGAAGAGCCTGTCCATACGGCACAGACAGGTT1150.1023341075131032No Hit
CGATAGGGGTACTACAGTGATATAAAAACCAGCAGTTTTTACACTAAATTTT1150.1023341075131032No Hit
TCAAATGGGGAACTGTATCGTCACACTATTTTTTAATTTCAGTAAGTTCTTC1140.10144424570864144No Hit
TGCTTAGGGATTCATTATCTTCTAGAAACCAAGAGTGGACTTGGTATCCGTC1140.10144424570864144No Hit
GAAACTGGGAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGG1140.10144424570864144No Hit
GCTTTAGGGCAGTAATTTATTCTTTTAGCAAACCTGATACTGATAACTGTTT1130.10055438390417967No Hit
GTAGGCGGGATATAATCTCCTGGTGTGCCATTTTTTAAGCCCGTTGGAAAAG1130.10055438390417967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)