FastQCFastQC Report
Sat 31 Dec 2022
SRR5846401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99118
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT8810.8888395649629734No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC7990.8061098892229464No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT2300.2320466514659295No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA1460.1472991787566335No Hit
TTTGCAGGGGGGTGATGAAGTTTCCAACACATGAAATTTGGGGGACACATTA1230.12409451361004055No Hit
AACAAGGGGAATTGACTTCCCTGAATTTCTGACAATGATGGCAAGAAAAATG1220.12308561512540607No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1200.12106781815613714No Hit
TTATTTGGGTACACGACGCTCTTCTGATCTATCTGTGGGAGTATGTCAAATG1190.12005891967150266No Hit
GTAGTTGGGGCAAACTGTCTGGTAAGAAACAGGAAGCTTAGTTTGACATCTG1160.11703222421759922No Hit
TGAACTGGCTCTCAGTTAAAAAACTGCTTGGATCTTTGTTTCTCTCTACAAC1150.11602332573296475No Hit
GTGTTAGGGCATTTTCAGTTATGTGAATGGCTCCATACAGCAAAAGAACCAT1100.11097883330979236No Hit
TAGCAAGGGGGGGCGCCGCGGGGCGGAAGACGAGGGCGGCGAGGTCGGGTTC1100.11097883330979236No Hit
CTTTCGGGGTCCTCTAGGCAGAGAAGAGGCGATGGCGGCGATGGCATCTCTC1080.10896103634052343No Hit
CAACATGGAGTAATAAGGATTGGATTTACCCTTCTATCTAAAGCAATCAAAA1070.10795213785588895No Hit
GCGTACGGGGTGATGGGAGAGGGGTCATGGTTAAGAAGATGTTTCTGGCTAG1070.10795213785588895No Hit
AGAAGGGATGCCGGTGACCTGAGGACTCCACTCACACTGGCGAGCAAAAAGG1060.10694323937125447No Hit
GTCTACGGGTGGTCCAGACCTGTACATGAATAACTTTGGTTAGTCAGAGTGA1040.10492544240198551No Hit
TTGGTGGGGATTGATACTAGAAATGAGTTGGAAAGCTATGCCTATTCTCTAA1030.10391654391735104No Hit
GTAACTGGGCCAGATACTTCCAGATTTAGAAATGTGTCAAAGGAAAAATTGG1020.10290764543271656No Hit
TTGTCAGGGCACGTGCATCATCTGGTTGGCCTTCCTCCCTATATTTTATGTG1010.10189874694808208No Hit
GTTAGAGGGGCGTTGAGTCACCATGACCAACTCATTATTTTTGAATTACCTA1000.1008898484634476No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)