FastQCFastQC Report
Sat 31 Dec 2022
SRR5846400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99963
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT9330.9333453377749767No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC8040.8042975901083401No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT1540.15405700109040343No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA1460.14605403999479807No Hit
AACAAGGGGAATTGACTTCCCTGAATTTCTGACAATGATGGCAAGAAAAATG1360.13605033862529137No Hit
TTATTTGGGTACACGACGCTCTTCTGATCTATCTGTGGGAGTATGTCAAATG1240.1240458969818833No Hit
CTTTCGGGGTCCTCTAGGCAGAGAAGAGGCGATGGCGGCGATGGCATCTCTC1230.12304552684493263No Hit
TCTTTAGGGAGATGTAAAGTGTCATTTTCATCACATCATATCAAGGGTACAC1210.12104478657103128No Hit
TGAACTGGCTCTCAGTTAAAAAACTGCTTGGATCTTTGTTTCTCTCTACAAC1160.11604293588627793No Hit
TTGCATGGGATCCCCCAAATCCAGATTCACCAGAAGTTCTATTATGTATGAA1150.11504256574932725No Hit
TAAGCTGGGGATATTTATGCAGAGAAAATTTTCTAGAAGGATAACATGCCAA1130.11304182547542592No Hit
CTTGCTAGGGCGATCTTACGACGGGGTGTGGTCGCGTCTCGGAAACCGGTAG1120.11204145533847525No Hit
TTGGTGGGGATTGATACTAGAAATGAGTTGGAAAGCTATGCCTATTCTCTAA1120.11204145533847525No Hit
GACTTTGGGACCTCCAGCCTATTAGGTCATTTCTGATTTGGGATTCGTCCTA1110.11104108520152457No Hit
TAGCAAGGGGGGGCGCCGCGGGGCGGAAGACGAGGGCGGCGAGGTCGGGTTC1110.11104108520152457No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1080.10803997479067254No Hit
TTTGCAGGGGGGTGATGAAGTTTCCAACACATGAAATTTGGGGGACACATTA1080.10803997479067254No Hit
AGAAGGGATGCCGGTGACCTGAGGACTCCACTCACACTGGCGAGCAAAAAGG1030.10303812410591918No Hit
GCTCACAGGGTGCCATTGTTTGAAGAACGACATCTACCCTGTAGTCATATGT1010.10103738383201784No Hit
GTCTACGGGTGGTCCAGACCTGTACATGAATAACTTTGGTTAGTCAGAGTGA1000.10003701369506718No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)