FastQCFastQC Report
Sat 31 Dec 2022
SRR5846399.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846399.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101719
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT8750.8602129395688121No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC7750.7619028893323765No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT2060.20251870348705747No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA1470.14451577384756042No Hit
GTAGTTGGGGCAAACTGTCTGGTAAGAAACAGGAAGCTTAGTTTGACATCTG1180.1160058592789941No Hit
TGAACTGGCTCTCAGTTAAAAAACTGCTTGGATCTTTGTTTCTCTCTACAAC1140.11207345726953666No Hit
AACAAGGGGAATTGACTTCCCTGAATTTCTGACAATGATGGCAAGAAAAATG1140.11207345726953666No Hit
TAAGCTGGGGATATTTATGCAGAGAAAATTTTCTAGAAGGATAACATGCCAA1130.11109035676717231No Hit
TAGCAAGGGGGGGCGCCGCGGGGCGGAAGACGAGGGCGGCGAGGTCGGGTTC1110.1091241557624436No Hit
GTGTTAGGGCATTTTCAGTTATGTGAATGGCTCCATACAGCAAAAGAACCAT1100.10814105526007925No Hit
CTTTCGGGGTCCTCTAGGCAGAGAAGAGGCGATGGCGGCGATGGCATCTCTC1090.10715795475771488No Hit
GCTTGCGGGGTGAAGACGAGTTAACTACTCATCCCAGAAATTACACTGCATT1070.10519175375298616No Hit
TTGGTGGGGATTGATACTAGAAATGAGTTGGAAAGCTATGCCTATTCTCTAA1070.10519175375298616No Hit
TTATTTGGGTACACGACGCTCTTCTGATCTATCTGTGGGAGTATGTCAAATG1060.10420865325062181No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1040.1022424522458931No Hit
GTAACTGGGCCAGATACTTCCAGATTTAGAAATGTGTCAAAGGAAAAATTGG1040.1022424522458931No Hit
GATCTTGGGTACACACCCCATCTTGGGAAAGAGTCACCAGTCTGACCTTGTG1040.1022424522458931No Hit
GTCTACGGGTGGTCCAGACCTGTACATGAATAACTTTGGTTAGTCAGAGTGA1040.1022424522458931No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)