FastQCFastQC Report
Sat 31 Dec 2022
SRR5846389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48790
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC33816.9296987087517925No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT24605.042016806722689No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT6721.3773314203730271No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA5261.0780897724943637No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2160.4427136708341873No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC2120.4345152695224431No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2020.4140192662430826No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1950.39967206394753024No Hit
ACTACAGGGTGGTTTCCTTTTGTTTCATGCTTGTGAGTGTGTGCTTGTGTTT1880.3853248616519778No Hit
TCTCAAGGGCGATCTTTTCCTGGGTGAATCTGTGAGCTTGGATGAGATCACT1850.3791760606681697No Hit
ATAGCGGGTTACCCAAAGACATTAAATCAACTATCTTAAGTATGTTCAGCGA1760.3607296577167452No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1560.31973765115802416No Hit
TTTAAAGGGGCACTGGTTAAAGTACAGTTTCCAACAGCTGTCCTTCCTCAGT1510.3094896495183439No Hit
ATTGCTGGGATTCATGTAATGGCATGTAGTATGCATTCAGATTCTTTTTTTT1450.29719204755072764No Hit
TTACATGGGGTGACTGCATCACTTTCTAAAATTTTGCTTAAAATTTGTACAT1370.2807952449272392No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1330.27259684361549497No Hit
CTATATAGGGTGAAGGAATAGAACCTGACCACATTATTATGAAACATTATGG1240.2541504406640705No Hit
CATTGCGGGATAAAGATGGCGATGGCACCATCACAACAAAGGAACTTGGAAC1190.24390243902439024No Hit
GAATAGGGACCTAGGCAAGAGGCAGGTCAACGGCACATGCGATAGGCTTAAT1150.23570403771264603No Hit
ATAGGTGGGACATGAAAGGTCTTCCTTGATAAACGATAAACCTTGTGATTTT1140.23365443738470998No Hit
TTACACGGGGATATAATTTAGTACTATAAGTTACTTAATAGCTGTCGTATTT1120.22955523672883787No Hit
ACGCGTGGGATCCTCATCAGCATTTGTTATTATCCTCTGTTTTGTTTTGTTT1050.2152080344332855No Hit
TATGTGGGGTGATTTACACATTATTAAAAAGGCAGTAAAATTTGAACTTATA1050.2152080344332855No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG1050.2152080344332855No Hit
TAAGCCGGGTCTTTCGCAGGGAGCAGACAAAGCAAAATTTTTATGTCAAAGA1030.21110883377741338No Hit
TCTTTAGGGATAGAAGAATTCACACAGGAGAGAAACCATATGAATGTAATCA990.2029104324656692No Hit
CAGTAAGGGATGAGGAAAAGAAAATCTTTTTAAAAATGATTTCAAGGTTTGT980.20086083213773312No Hit
TTACAGGGTAGTATAGTTGTAACTTATTACAGGATCATACTAAAATTTCTGT940.1926624308259889No Hit
CTGTGGGGGAGTGGGGAAGGTGTCAATGGGGAGTGTCACGGACAGGAGGTAG920.18856323017011684No Hit
GCATCTGGGATGGTTAGTCTTTGATTTCTTGTGCAGACAGAGAGAAGCGTGA910.18651362984218078No Hit
CGCCTGGGGAGGAAGATACTTTGGGGATTGTAAAGAGGAAGAACTGTTGCCC880.1803648288583726No Hit
GTCTTGGGGGAATTTTAAGAGCTTTATTATCAGTTTAACCCTCCCTAGTTGA810.16601762656282026No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC780.15986882557901208No Hit
AACTTAGGGCTAATGTAATAAAGTCAGACAAAATGCATACTTTATAGTTTCA780.15986882557901208No Hit
TGCTGGGAAAACTGGATAATCCATATGCAGAAAAATGATACTAGACCCCTAT770.15781922525107603No Hit
ATTTCTGGGGAAAGAACTCAAGTGTGACTACAATGAAAGGATAAGTGTTCTC750.15372002459520395No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTACCTCGTATGC740.15167042426726787No Hit
ATACAGAGGGTGGAAGCAAGTGTGAGGGTGTGAATTAATCATTCTTTCTTTC740.15167042426726787No Hit
AATCAAGGGTCTTTTTACATTTGTTTGTAAACATGTTTAAAGAACGAACCTA720.14757122361139577No Hit
TCATTGGGGGACTATAGTGAGCTTTAGCAAAAGTTTTTCTATATAATGACAT680.13937282229965156No Hit
GGCTTGGGAGATGTTCCATATCTTCAAAATGCCTCCTCCAATTTTGTAAGAA670.1373232219717155No Hit
GCTACAGGGAGACGTTTATTGGGATACCTTTTACTTGGACAATATGTTAGCA660.13527362164377946No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT640.13117442098790735No Hit
ACACTGAGGTAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAAC630.1291248206599713No Hit
CCCTTCGGGTCCTGTGTCTGGAATACCCCCATTCTCCCATGCTCAGCCCCGT630.1291248206599713No Hit
TACACTGGGACAACTGAATCTAAAATTTGCAATCAATATAATCATGGATCAC630.1291248206599713No Hit
GTAATCGGGGTATAAGCAGTGATTTTTTTTTTAAACTTTCTATATTTTAATT630.1291248206599713No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTACCTCGTATGCCGTCT620.12707522033203525No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATG600.12297601967616315No Hit
ATATATGGGTGCTTGCATTTTTTAAAAACTAGACATTGTGGCTTGAGAATTT600.12297601967616315No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATA600.12297601967616315No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC600.12297601967616315No Hit
AAAAAAGGGACTGTGAAACATATCCTCTTGTTCTGTATCCTCTCCAACACTT590.12092641934822711No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGCCGTCT590.12092641934822711No Hit
CTAAAAGGGTTATGAAATTAATCAAAGTAGTTAAACAGAAAGTGAAAATGTG580.11887681902029104No Hit
TTTGCAGGGATGGTTGATAAATTATCAATATCATCTAAAGTTTGATGAAGTA580.11887681902029104No Hit
CTCTTGGGGGTATAAAACTATACCCTGGACCCCCACGGTGGTTGTTATGGTC560.11477761836441894No Hit
TCAGGGGTATGTTGAAACACGAATCCCAAGGTGATGGTATTTGAAGGTAGGG530.10862881738061078No Hit
TATTAGGGGATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGC530.10862881738061078No Hit
GATAATGGGCTGGAGGTTAGGGGATATTTATGGTTGCTGGTGATAACTTTGT520.10657921705267473No Hit
AATACTGGGAGGCACTTCACAGTTTACTTGAAAGAGGCTTTGGAAAATAGAT510.10452961672473868No Hit
TAAAATGGGATGGCTGCAAGTCTCTGCTAATTACTCCTATGCCACTTTCAGC510.10452961672473868No Hit
AAAGCTGGGATGGGTGGCACAAATCTGAATAGGTTTTGCTGCCAATACACAT490.10043041606886656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)