FastQCFastQC Report
Sat 31 Dec 2022
SRR5846388.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846388.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47837
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC33036.904697200911429No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT25765.384953069799527No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT5981.2500783912034619No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4991.043125614064427No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2310.4828898133244141No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2110.4410811714781446No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT2060.43062901101657713No Hit
TCTCAAGGGCGATCTTTTCCTGGGTGAATCTGTGAGCTTGGATGAGATCACT2000.41808641846269623No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1730.36164475197023227No Hit
ACTACAGGGTGGTTTCCTTTTGTTTCATGCTTGTGAGTGTGTGCTTGTGTTT1710.3574638877856053No Hit
ATTGCTGGGATTCATGTAATGGCATGTAGTATGCATTCAGATTCTTTTTTTT1600.334469134770157No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1540.3219265422162761No Hit
TTACATGGGGTGACTGCATCACTTTCTAAAATTTTGCTTAAAATTTGTACAT1400.29266049292388735No Hit
ATAGCGGGTTACCCAAAGACATTAAATCAACTATCTTAAGTATGTTCAGCGA1370.2863891966469469No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1340.2801179003700065No Hit
TTTAAAGGGGCACTGGTTAAAGTACAGTTTCCAACAGCTGTCCTTCCTCAGT1210.2529422831699312No Hit
CTGTGGGGGAGTGGGGAAGGTGTCAATGGGGAGTGTCACGGACAGGAGGTAG1210.2529422831699312No Hit
CTATATAGGGTGAAGGAATAGAACCTGACCACATTATTATGAAACATTATGG1150.24039969061605032No Hit
TAAGCCGGGTCTTTCGCAGGGAGCAGACAAAGCAAAATTTTTATGTCAAAGA1080.22576666596985598No Hit
GAATAGGGACCTAGGCAAGAGGCAGGTCAACGGCACATGCGATAGGCTTAAT1070.2236762338775425No Hit
TTACAGGGTAGTATAGTTGTAACTTATTACAGGATCATACTAAAATTTCTGT1040.21740493760060206No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG1020.2132240734159751No Hit
TATGTGGGGTGATTTACACATTATTAAAAAGGCAGTAAAATTTGAACTTATA990.20695277713903465No Hit
AATCAAGGGTCTTTTTACATTTGTTTGTAAACATGTTTAAAGAACGAACCTA980.20486234504672116No Hit
ATAGGTGGGACATGAAAGGTCTTCCTTGATAAACGATAAACCTTGTGATTTT940.1965006166774672No Hit
CATTGCGGGATAAAGATGGCGATGGCACCATCACAACAAAGGAACTTGGAAC930.19441018458515374No Hit
AACTTAGGGCTAATGTAATAAAGTCAGACAAAATGCATACTTTATAGTTTCA910.19022932040052679No Hit
TCTTTAGGGATAGAAGAATTCACACAGGAGAGAAACCATATGAATGTAATCA880.18395802412358636No Hit
GCATCTGGGATGGTTAGTCTTTGATTTCTTGTGCAGACAGAGAGAAGCGTGA860.17977715993895937No Hit
GTCTTGGGGGAATTTTAAGAGCTTTATTATCAGTTTAACCCTCCCTAGTTGA850.1776867278466459No Hit
TTACACGGGGATATAATTTAGTACTATAAGTTACTTAATAGCTGTCGTATTT830.17350586366201892No Hit
CAGTAAGGGATGAGGAAAAGAAAATCTTTTTAAAAATGATTTCAAGGTTTGT820.17141543156970546No Hit
TTTGCAGGGATGGTTGATAAATTATCAATATCATCTAAAGTTTGATGAAGTA820.17141543156970546No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC810.16932499947739196No Hit
CTAAAAGGGTTATGAAATTAATCAAAGTAGTTAAACAGAAAGTGAAAATGTG790.16514413529276503No Hit
ATTTCTGGGGAAAGAACTCAAGTGTGACTACAATGAAAGGATAAGTGTTCTC770.16096327110813805No Hit
GATAATGGGCTGGAGGTTAGGGGATATTTATGGTTGCTGGTGATAACTTTGT710.14842067855425717No Hit
TGCTGGGAAAACTGGATAATCCATATGCAGAAAAATGATACTAGACCCCTAT700.14633024646194367No Hit
ACGCGTGGGATCCTCATCAGCATTTGTTATTATCCTCTGTTTTGTTTTGTTT680.14214938227731672No Hit
CGCCTGGGGAGGAAGATACTTTGGGGATTGTAAAGAGGAAGAACTGTTGCCC680.14214938227731672No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATG670.14005895018500325No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCG670.14005895018500325No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTAT660.13796851809268976No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATA640.1337876539080628No Hit
GCTACAGGGAGACGTTTATTGGGATACCTTTTACTTGGACAATATGTTAGCA640.1337876539080628No Hit
TATTCGGGGCAGAACAGGGAGCTAACAGTAAATAGCCCATTATCAGTTACAT630.1316972218157493No Hit
TGGTTGGGCGGGCGTGGTGGCTCACGCCTGTGGTCTCAGCACTTTTGAAGGC630.1316972218157493No Hit
ATACAGAGGGTGGAAGCAAGTGTGAGGGTGTGAATTAATCATTCTTTCTTTC630.1316972218157493No Hit
ACACTGAGGTAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAAC620.12960678972343584No Hit
TACACTGGGACAACTGAATCTAAAATTTGCAATCAATATAATCATGGATCAC620.12960678972343584No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC600.12542592553880888No Hit
GTAATCGGGGTATAAGCAGTGATTTTTTTTTTAAACTTTCTATATTTTAATT600.12542592553880888No Hit
ATATATGGGTGCTTGCATTTTTTAAAAACTAGACATTGTGGCTTGAGAATTT560.11706419716955493No Hit
TATTAGGGGATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGC560.11706419716955493No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTACCTCGTATGC560.11706419716955493No Hit
ATAGTCGGGTCTGTTTATTTGTCTTCTTTGATTTTTTTCCATGACTGTTGTA540.11288333298492799No Hit
TAAAATGGGATGGCTGCAAGTCTCTGCTAATTACTCCTATGCCACTTTCAGC530.11079290089261451No Hit
TCATTGGGGGACTATAGTGAGCTTTAGCAAAAGTTTTTCTATATAATGACAT520.10870246880030103No Hit
TTCTAGGGAGAGTTTAGGCTCAAAAGAGTTGGTGGACTGAGATTGAAATTTG500.10452160461567406No Hit
AATACTGGGAGGCACTTCACAGTTTACTTGAAAGAGGCTTTGGAAAATAGAT490.10243117252336058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)