FastQCFastQC Report
Sat 31 Dec 2022
SRR5846387.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846387.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48158
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC33246.902279995016404No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT24715.131027036006479No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT6081.2625109016155156No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4650.9655716599526558No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2390.4962830682337306No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC2170.45060010797790606No Hit
ACTACAGGGTGGTTTCCTTTTGTTTCATGCTTGTGAGTGTGTGCTTGTGTTT2040.42360563146310065No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT2010.4173761368827609No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1990.4132231404958678No Hit
TCTCAAGGGCGATCTTTTCCTGGGTGAATCTGTGAGCTTGGATGAGATCACT1940.4028406495286349No Hit
ATTGCTGGGATTCATGTAATGGCATGTAGTATGCATTCAGATTCTTTTTTTT1710.35508119107936376No Hit
ATAGCGGGTTACCCAAAGACATTAAATCAACTATCTTAAGTATGTTCAGCGA1570.32601021637111177No Hit
TTACATGGGGTGACTGCATCACTTTCTAAAATTTTGCTTAAAATTTGTACAT1420.2948627434694132No Hit
CTGTGGGGGAGTGGGGAAGGTGTCAATGGGGAGTGTCACGGACAGGAGGTAG1360.28240375430873377No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1330.27617425972839404No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1280.2657917687611612No Hit
CTATATAGGGTGAAGGAATAGAACCTGACCACATTATTATGAAACATTATGG1180.2450267868266955No Hit
GAATAGGGACCTAGGCAAGAGGCAGGTCAACGGCACATGCGATAGGCTTAAT1160.24087379043980234No Hit
TTTAAAGGGGCACTGGTTAAAGTACAGTTTCCAACAGCTGTCCTTCCTCAGT1150.23879729224635574No Hit
ATAGGTGGGACATGAAAGGTCTTCCTTGATAAACGATAAACCTTGTGATTTT1130.23464429585946261No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG1100.22841480127912286No Hit
TCTTTAGGGATAGAAGAATTCACACAGGAGAGAAACCATATGAATGTAATCA1090.22633830308567632No Hit
TTACAGGGTAGTATAGTTGTAACTTATTACAGGATCATACTAAAATTTCTGT1080.22426180489222972No Hit
GTCTTGGGGGAATTTTAAGAGCTTTATTATCAGTTTAACCCTCCCTAGTTGA1060.22010880850533662No Hit
TAAGCCGGGTCTTTCGCAGGGAGCAGACAAAGCAAAATTTTTATGTCAAAGA1030.21387931392499687No Hit
CGCCTGGGGAGGAAGATACTTTGGGGATTGTAAAGAGGAAGAACTGTTGCCC980.20349682295776403No Hit
CAGTAAGGGATGAGGAAAAGAAAATCTTTTTAAAAATGATTTCAAGGTTTGT930.19311433199053116No Hit
TTACACGGGGATATAATTTAGTACTATAAGTTACTTAATAGCTGTCGTATTT910.18896133560363804No Hit
TGCTGGGAAAACTGGATAATCCATATGCAGAAAAATGATACTAGACCCCTAT910.18896133560363804No Hit
ACGCGTGGGATCCTCATCAGCATTTGTTATTATCCTCTGTTTTGTTTTGTTT900.18688483741019146No Hit
TATGTGGGGTGATTTACACATTATTAAAAAGGCAGTAAAATTTGAACTTATA900.18688483741019146No Hit
AATCAAGGGTCTTTTTACATTTGTTTGTAAACATGTTTAAAGAACGAACCTA890.1848083392167449No Hit
CATTGCGGGATAAAGATGGCGATGGCACCATCACAACAAAGGAACTTGGAAC880.1827318410232983No Hit
AACTTAGGGCTAATGTAATAAAGTCAGACAAAATGCATACTTTATAGTTTCA880.1827318410232983No Hit
GCATCTGGGATGGTTAGTCTTTGATTTCTTGTGCAGACAGAGAGAAGCGTGA870.18065534282985174No Hit
CTAAAAGGGTTATGAAATTAATCAAAGTAGTTAAACAGAAAGTGAAAATGTG830.17234935005606544No Hit
ATTTCTGGGGAAAGAACTCAAGTGTGACTACAATGAAAGGATAAGTGTTCTC810.1681963536691723No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC760.15781386270193945No Hit
ATATATGGGTGCTTGCATTTTTTAAAAACTAGACATTGTGGCTTGAGAATTT750.15573736450849288No Hit
TATTAGGGGATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGC750.15573736450849288No Hit
CTCTTGGGGGTATAAAACTATACCCTGGACCCCCACGGTGGTTGTTATGGTC740.1536608663150463No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATA720.14950786992815318No Hit
TGGTTGGGCGGGCGTGGTGGCTCACGCCTGTGGTCTCAGCACTTTTGAAGGC710.1474313717347066No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATG660.13704888076747374No Hit
ATAGTCGGGTCTGTTTATTTGTCTTCTTTGATTTTTTTCCATGACTGTTGTA660.13704888076747374No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT620.12874288799368744No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGCCGTCT620.12874288799368744No Hit
ACACTGAGGTAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAAC610.12666638980024086No Hit
TTTGCAGGGATGGTTGATAAATTATCAATATCATCTAAAGTTTGATGAAGTA590.12251339341334774No Hit
TACACTGGGACAACTGAATCTAAAATTTGCAATCAATATAATCATGGATCAC590.12251339341334774No Hit
GATAATGGGCTGGAGGTTAGGGGATATTTATGGTTGCTGGTGATAACTTTGT580.12043689521990117No Hit
AAAGCTGGGATGGGTGGCACAAATCTGAATAGGTTTTGCTGCCAATACACAT580.12043689521990117No Hit
TATTCGGGGCAGAACAGGGAGCTAACAGTAAATAGCCCATTATCAGTTACAT570.1183603970264546No Hit
TCATTGGGGGACTATAGTGAGCTTTAGCAAAAGTTTTTCTATATAATGACAT560.116283898833008No Hit
ATACAGAGGGTGGAAGCAAGTGTGAGGGTGTGAATTAATCATTCTTTCTTTC560.116283898833008No Hit
AAAAAAGGGACTGTGAAACATATCCTCTTGTTCTGTATCCTCTCCAACACTT550.11420740063956143No Hit
TAAGGTGGGATAACTAATATTTATGGAATCTACACTGTGTTTCAGCTACTGT530.11005440425266831No Hit
GGCTTGGGAGATGTTCCATATCTTCAAAATGCCTCCTCCAATTTTGTAAGAA530.11005440425266831No Hit
GTAATCGGGGTATAAGCAGTGATTTTTTTTTTAAACTTTCTATATTTTAATT520.10797790605922174No Hit
AACTACGGGTGGGACCTCAGTCTTCTTCAGCAGAGGACTTGATATTTTGTAT490.10174841147888201No Hit
GAGGCAGGAGCATTGCTTGAGCCTGAGACGTGGAGGCTGCGGTGAGCTAAGA490.10174841147888201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)