FastQCFastQC Report
Sat 31 Dec 2022
SRR5846380.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846380.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189739
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC33851.7840296407169849No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT28991.5278883097307354No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT4710.24823573435087146No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3690.19447767723030057No Hit
CTTGCTGGGGGCACCGGAAGCCGACTCAACAGAGCTATGGCGGGTTTGACTG2650.13966554055834593No Hit
TTATGAGGGAGGTCAAAGTCACTGGCTCAAGATCCTATGTATTAGCAGCAGA2420.12754362571743288No Hit
AATTTGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCCC2380.12543546661466543No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2340.12332730751189792No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2310.12174618818482232No Hit
AGGTCCGGGCTAGGCGGGCGGATTACCTGAGGTTAGGAGTTCAAGACCAGCA2300.12121914840913044No Hit
GAGTCTGGGACTTCTAGGCTTTTTCAGGATGCTGGAAGAATTCATTTCTCTG2200.11594875065221173No Hit
TAGTAAGGGTAGTGTGAATATTTTGTTAATTAGATGTAGCAGAAAAATAACG2160.11384059154944423No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC2150.11331355177375237No Hit
CCAGAAGGTATTGATCTGTTCAGGTTTTTGATTTCTTCATGGTTCAGTCCTG2140.11278651199806049No Hit
AATGTGTGGGGTACATGGTAAATTCTTAATGAATATTTTTACAATGAATGAT2080.10962427334390927No Hit
AAACGCGGGAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATT2080.10962427334390927No Hit
TGACATAGGGGTACTAAAATCTTGCATTTTATTGAGAGCCACCATTAAACCC2060.10857019379252551No Hit
ATTAACGGGGGACTTATTCTTGTATGGAATTTGACATTTTTACCTTGGCTCA2020.10646203468975804No Hit
GGGCAAGGGGGTACTTACTGTAGCTACACATATTAAAAGGCAAAGTGTTTCT2020.10646203468975804No Hit
CATAAATGGGATAGTATTATTTTAAACTTTTCATTATCTGTGATCGTGGCTC2010.10593499491406617No Hit
CACACAGGGTCAGGTGGGGAATGTGTGCATATTGTTGACAGAATTTGAAGGC2010.10593499491406617No Hit
AAACAGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA2010.10593499491406617No Hit
AGTACGGGATTTCTTGAATTAGCCAAAATTATGAGGTATCGACATGAATTCC2000.1054079551383743No Hit
TTTTGAGGGTACACACCATATAGAAATATATTCCAGATGATAAAAACATTAA1990.10488091536268243No Hit
TTTTGCGGGAAAATCCTGGTTGGGATATTGTACTGTAGTTTAGCAAGATGTG1990.10488091536268243No Hit
TAACGGGGCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAG1990.10488091536268243No Hit
ATAACCGGGAACGAGACAGAAGTGAAAGGAAAACTGGTGCCGGTATTACTGT1990.10488091536268243No Hit
TTTGAAGGGGTCCGTTTTCTCTAGGTTTTCTAATTTAGGTACGTAAAGGTGT1980.10435387558699054No Hit
ACAGCAGGGTGGGATTTTGTTTTCTTTTAAAGAGACAGTGATCGTGTTTTTC1960.1032997960356068No Hit
TATAGTGGGATTTCAATGTTTGTTGAATAGCTCCAATGAATATCCCCCGGAG1940.10224571648422306No Hit
CCTGACCTCGTGATCTGCCTGCCTCGGCCTCCCAAGGTGCTGGGATTGCAGG1930.1017186767085312No Hit
GTTCGCGGGAAACTAAAACCAGGTGACTGGTCTCAGCAAGACATAGGTACTA1910.10066459715714746No Hit
TTCCCTTCCCCAGCCTCCAGTCCAGAATTCATCCTGCATCACACAGTGCATC1910.10066459715714746No Hit
TTTTGAGGGGATCCTGTAAATTCTAAGATTTTAAAAGGATTTTTGCTTATGT1910.10066459715714746No Hit
ACAGACGGGGAAGGAAGGAGAGAGAAAGAAAAGAAAGAAAGAAAGAAGAGAA1900.10013755738145559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)