FastQCFastQC Report
Sat 31 Dec 2022
SRR5846379.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846379.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189633
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC34171.8019015677651042No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT27551.4528062098896288No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT5260.2773778825415408No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4230.22306244166363448No Hit
CTTGCTGGGGGCACCGGAAGCCGACTCAACAGAGCTATGGCGGGTTTGACTG2600.1371069381384042No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2500.13183359436385017No Hit
AGGTCCGGGCTAGGCGGGCGGATTACCTGAGGTTAGGAGTTCAAGACCAGCA2480.1307789256089394No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2340.12339624432456375No Hit
CTTAAGGGATCTTTTGTAATGTCATTCATAGAAAACTGGTAAATGTGTTTCC2330.12286890994710835No Hit
TAGTAAGGGTAGTGTGAATATTTTGTTAATTAGATGTAGCAGAAAAATAACG2270.11970490368237595No Hit
TTAAATGGGAGTCAATAAAAGCTTTAAAGTTAAGTCTCAAAGGCTGTGAAAA2240.11812290055000976No Hit
ATTAACGGGGGACTTATTCTTGTATGGAATTTGACATTTTTACCTTGGCTCA2230.11759556617255434No Hit
CATATAGGGCTCTGAACAAGAAAAAACCCAAACAAACATAAAATAGTAGTGG2220.11706823179509895No Hit
TTTTGAGGGGATCCTGTAAATTCTAAGATTTTAAAAGGATTTTTGCTTATGT2220.11706823179509895No Hit
CCAGAAGGTATTGATCTGTTCAGGTTTTTGATTTCTTCATGGTTCAGTCCTG2200.11601356304018816No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC2170.11443155990782194No Hit
ACAGCAGGGTGGGATTTTGTTTTCTTTTAAAGAGACAGTGATCGTGTTTTTC2130.11232222239800035No Hit
CTATGTGGGAGAAGAAATAAAGCTTTTCTTTGATTTCTCAGCTGAAGTGTTG2120.11179488802054495No Hit
AATTTGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCCC2110.11126755364308955No Hit
TTTTGCGGGAAAATCCTGGTTGGGATATTGTACTGTAGTTTAGCAAGATGTG2100.11074021926563414No Hit
AGACATGGGAATTTCTCTGTGAAACACAGTTACCATCCTCACCCCGGGGCTC2090.11021288488817875No Hit
TAACGGGGCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAG2070.10915821613326794No Hit
AATGTGTGGGGTACATGGTAAATTCTTAATGAATATTTTTACAATGAATGAT2060.10863088175581254No Hit
TTATGAGGGAGGTCAAAGTCACTGGCTCAAGATCCTATGTATTAGCAGCAGA2060.10863088175581254No Hit
GGGCAAGGGGGTACTTACTGTAGCTACACATATTAAAAGGCAAAGTGTTTCT2050.10810354737835713No Hit
TAGTTGGGGACAAAAATGTTATGGAATCAAGGATTTTAACTGTATATGCTTT2040.10757621300090175No Hit
TTGCACGGGATCTCAAAATGCTGAACTGCTCTTTGGAAGTCGCCGGTGCTGT2040.10757621300090175No Hit
TAGCCAGGGACTTTGGGCTTTGGGGTAATAGATTCCATATCAATATATATCT2040.10757621300090175No Hit
TTTGAAGGGGTCCGTTTTCTCTAGGTTTTCTAATTTAGGTACGTAAAGGTGT2040.10757621300090175No Hit
TACATGGGGCCTAATATCACTTGGATGTTGGTGGGTTTTTGAATTTTTGGGT2030.10704887862344635No Hit
TATAGTGGGATTTCAATGTTTGTTGAATAGCTCCAATGAATATCCCCCGGAG2030.10704887862344635No Hit
ACAGACGGGGAAGGAAGGAGAGAGAAAGAAAAGAAAGAAAGAAAGAAGAGAA2010.10599420986853554No Hit
AATTGAGGGTAAGTGAAAAACCTTTGTGTAAAACAGGTGAGTGGAGAAGACG1990.10493954111362473No Hit
GAGTCTGGGACTTCTAGGCTTTTTCAGGATGCTGGAAGAATTCATTTCTCTG1990.10493954111362473No Hit
TTCCCTTCCCCAGCCTCCAGTCCAGAATTCATCCTGCATCACACAGTGCATC1990.10493954111362473No Hit
TAATAGGGGGAATGACCTTCTTGTCTCTTTTTATTGTTTTTGACTTAAAATC1980.10441220673616934No Hit
ATGAGGGTACGGAAATTCTAGGCACTAACACATTAAAAGTATCCTTTTGGGA1970.10388487235871394No Hit
TACTCTGGGGAGTTGGATAACTTAGATGAAATAAACACATCTCTTAAAAAAG1960.10335753798125853No Hit
CTATCAGGGATTCTTTAATGAGCATCCCTTCTTTAAAAAAACCTAATTGCCA1960.10335753798125853No Hit
CAATACGGGATGGGCTCAAATTCTCTACCTCTTCAGGGCTAATACTTTTAAC1960.10335753798125853No Hit
AAACAGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1960.10335753798125853No Hit
AGCAGGGGGAGCATGAAGGGGTGTTGAATTTTATTGAAGGCCTTTTCTGCAT1950.10283020360380313No Hit
TCGTCGAGGGAATGACTACTTGCCATCTTCTTTGTGTTTTCTTTCCAGACTG1940.10230286922634772No Hit
AAACGCGGGAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATT1940.10230286922634772No Hit
GTGCCAGGGAAGACCTATATTCTCTGTAGTTACTGAAAACCGAAAGTCAATT1920.10124820047143694No Hit
TACATTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1910.10072086609398154No Hit
CTAACGGGGAACTAACTATATTTTAATAAAACAGAAAAGAAAATACAGCTTT1910.10072086609398154No Hit
GGCGAGGGGTATGTACAAAGGCAAACAGACACATAACAGTCTCTGCAGACTG1910.10072086609398154No Hit
ATGGAGGGCAGGGGTCATCGCCTCCTACCGTCGTTGCCTGCCCCAGATCCAG1900.10019353171652613No Hit
GTAAAAGGGCTCAACATCCAAGGCAGCTTATTCGAACTCTGCGGCAGCGGCA1900.10019353171652613No Hit
AAATAAGGGCACCAGCAGTTTTTTGCCATCGTCCTGCTCATTGGCACCCGCA1900.10019353171652613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)