FastQCFastQC Report
Sat 31 Dec 2022
SRR5846378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190860
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC33191.738970973488421No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT28221.4785706800796394No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT4820.25254112962380804No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3950.206957979670963No Hit
TTATGAGGGAGGTCAAAGTCACTGGCTCAAGATCCTATGTATTAGCAGCAGA2600.1362255056062035No Hit
AGGTCCGGGCTAGGCGGGCGGATTACCTGAGGTTAGGAGTTCAAGACCAGCA2550.13360578434454573No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC2490.13046211883055642No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2330.1220790107932516No Hit
CTTGCTGGGGGCACCGGAAGCCGACTCAACAGAGCTATGGCGGGTTTGACTG2300.12050717803625693No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2290.11998323378392538No Hit
TGTGACGGGGTCACAGATGTGAAGTCGATTTCACTTTATAACTAACCAGTCC2290.11998323378392538No Hit
TTCCCTTCCCCAGCCTCCAGTCCAGAATTCATCCTGCATCACACAGTGCATC2260.11841140102693073No Hit
CATATAGGGCTCTGAACAAGAAAAAACCCAAACAAACATAAAATAGTAGTGG2250.11788745677459918No Hit
ACAGACGGGGAAGGAAGGAGAGAGAAAGAAAAGAAAGAAAGAAAGAAGAGAA2240.11736351252226764No Hit
ATTAACGGGTAGCAATCAGCTTATTGGGAACAGTTGATTAACTGTAATAGAA2200.11526773551294144No Hit
AATTGAGGGTAAGTGAAAAACCTTTGTGTAAAACAGGTGAGTGGAGAAGACG2150.11264801425128367No Hit
CACACAGGGTCAGGTGGGGAATGTGTGCATATTGTTGACAGAATTTGAAGGC2150.11264801425128367No Hit
AATGTGTGGGGTACATGGTAAATTCTTAATGAATATTTTTACAATGAATGAT2140.11212406999895211No Hit
ACAGCAGGGTGGGATTTTGTTTTCTTTTAAAGAGACAGTGATCGTGTTTTTC2130.11160012574662057No Hit
TCTTTAGGGAGGAGTTATAGGCCAGGAAATAAATCCAAAGACCACATATTAA2110.11055223724195747No Hit
TTATATGGGTGACCAATGACTGGGAAGATCACTTGGCAGTGAAGCATTTTTC2080.1089804044849628No Hit
ATAACCGGGAACGAGACAGAAGTGAAAGGAAAACTGGTGCCGGTATTACTGT2070.10845646023263124No Hit
TATAGTGGGATTTCAATGTTTGTTGAATAGCTCCAATGAATATCCCCCGGAG2060.1079325159802997No Hit
GAGCAGGGGTATGTTATGCGTATACAAGGATGTACACATATATTTTGTAAAT2040.1068846274756366No Hit
TTTTGCGGGAAAATCCTGGTTGGGATATTGTACTGTAGTTTAGCAAGATGTG2030.10636068322330504No Hit
TGACATAGGGGTACTAAAATCTTGCATTTTATTGAGAGCCACCATTAAACCC2020.1058367389709735No Hit
AAACGCGGGAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATT2010.10531279471864194No Hit
TACATGGGGCCTAATATCACTTGGATGTTGGTGGGTTTTTGAATTTTTGGGT1990.10426490621397884No Hit
CCAGAAGGTATTGATCTGTTCAGGTTTTTGATTTCTTCATGGTTCAGTCCTG1980.10374096196164727No Hit
AAGCATGGGCAGCCTGGTCAATTTGTACCTGCAGGCCTCCTACACCTACCTC1970.10321701770931573No Hit
TAGCCAGGGACTTTGGGCTTTGGGGTAATAGATTCCATATCAATATATATCT1970.10321701770931573No Hit
TGGCAGGGGGTATCAGAGACTGGTGGTGGAGCTGCTTATCAAAAACGAAGAC1970.10321701770931573No Hit
AATTTGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCCC1960.10269307345698417No Hit
TTTGAAGGGGTCCGTTTTCTCTAGGTTTTCTAATTTAGGTACGTAAAGGTGT1960.10269307345698417No Hit
TGTTCAGGGACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC1950.10216912920465263No Hit
TACATTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1940.10164518495232107No Hit
TTTATAGGGCTAAGCATACTGATAATTAACCTCAAGTTAACAGTTTTAACAC1940.10164518495232107No Hit
CATAAATGGGATAGTATTATTTTAAACTTTTCATTATCTGTGATCGTGGCTC1940.10164518495232107No Hit
TGTCATGGGATAATGTAATTATTAAATTTAAGCTAACTTTCTACTGGCTATA1940.10164518495232107No Hit
GAGTCTGGGACTTCTAGGCTTTTTCAGGATGCTGGAAGAATTCATTTCTCTG1940.10164518495232107No Hit
CTTTTAGGGAATGTGTAAAATTTTCTGTATTTCACTTTGTCTTATTTGTAAA1940.10164518495232107No Hit
TAACGGGGCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAG1940.10164518495232107No Hit
AAATTTGGGATCCTTTGTGGTTTCTGTACAACCTGTAAAGACTCGTAAATAA1940.10164518495232107No Hit
GCTAGTGGGTCTAATGGGATGGGATGAGAAGGGCATGCTGCCTGTCTGGTAT1930.10112124069998953No Hit
GGGCAAGGGGGTACTTACTGTAGCTACACATATTAAAAGGCAAAGTGTTTCT1930.10112124069998953No Hit
TACAGCGGGCAGACAATGGTAAAAACTCTTGTCTAAAAAAAGAAATCTGAAA1920.10059729644765797No Hit
TACACAAGGGAAGACTTTATGAGCAGAAAGTTGGTATACTTGCTTTCTACAG1920.10059729644765797No Hit
AAACAGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1920.10059729644765797No Hit
TTTCGGGGTCTGTGGACATTTCTGGCCTCTCTAAAATTTCTTTACCATTTTT1920.10059729644765797No Hit
AGACATGGGAATTTCTCTGTGAAACACAGTTACCATCCTCACCCCGGGGCTC1910.10007335219532643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)