FastQCFastQC Report
Sat 31 Dec 2022
SRR5846377.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846377.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109153
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC26162.396635914725202No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT23892.188670948118696No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT4650.42600753071376873No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3620.33164457229760064No Hit
GTGCTGGGATATTATGATCTTGCTGCAGCCACAGTGCAGCTCCACATTAACT2020.1850613359229705No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC1930.17681602887689757No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1910.17498373842221468No Hit
GACGCAGGGCGATCTTTAAACGGGAGATTTTCCAATTTATTCACATATAGTT1770.16215770523943454No Hit
TTGATCCGGGAATCCACAGATTCACCACTTCATAAGTGGATAATCGAATTGT1760.1612415600120931No Hit
AAATACGGGAACCTACTTAGTTCCTTTGAAATGAAGTAAAGGTAATAGACAA1650.1511639625113373No Hit
TCCTTGGGGAATTGTTGATTGATTGAGAGTTATGATCTTCTTGTATTCTTTT1560.14291865546526436No Hit
TGACGCGGGACACACATGAGTGCAGGAGGGCAGACACAACACTCAGTTTTGT1500.13742178410121572No Hit
TCAATAGGGGGAAAACAGCGTGGAGATTCCTTAAAGAACTAAAAGTAGAACT1480.13558949364653286No Hit
AAGAGAGGGCCAAGAAACGGTGAGGATAGAAGGGGCCGTAAAATATCAAACA1460.13375720319184997No Hit
ATGACCGGGCTCCTGCAGCATATTGAACCAATCCACAGAGTGGATATGTCCA1460.13375720319184997No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1450.13284105796450854No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1440.13192491273716708No Hit
ATTTCAGGGATTTGAACAATAGTGTTTGCCATGGTTTGAATGATGGTATCTG1440.13192491273716708No Hit
ACAATGGGGCCTTACCAAGCAACTACTTCACTGTATGGCCTGCAACCAACTT1430.13100876750982565No Hit
CCTTTAGGGCTCATGGCAGTCAAGCAGAAGCAGCCCAGTTTCTCCCAGCAGA1380.12642804137311847No Hit
AACAAAGGGCATGCCCTTCCCTTTCCCTACCCCTGATAAGATCAAGTTGTTT1350.12367960569109415No Hit
TGCGTGGGGCATCTTTGTTGGATAAACAAAAATTTGAGGCTATGTAAATGAT1350.12367960569109415No Hit
TCAATGGGGGACGCTCTTCCGATCTTTGTAAGGGGAGGATGAAAATCCTTCT1340.12276346046375271No Hit
ATTCTAGGGAAGAACCTTTAGATATAAATGCATTTTAACTGTTCTGGAGAGT1330.12184731523641128No Hit
TTCCAGGGCACACCATGAATGTGCCCAAGGTAGTTAGGATTCTGAGAGCGTA1320.12093117000906983No Hit
TAATGTGGGACGCTCTTCTTTTCATTATTTCTAATGCTACAAGAATGCTGTA1310.12001502478172839No Hit
CCAGGTGGGTGAGTTCAAGCCGGATGTGCTCCTGTATTTAGCATGACAGGAA1300.11909887955438696No Hit
AAGAACGGGAATGATTTTGAAGGAAAGCTGATAACTATTTTGAGTTAAATTT1300.11909887955438696No Hit
GACGATGGGCAGAGGTGCTGGAGCTGGCAGGTAATGCTTCTAAGGATCTCAA1300.11909887955438696No Hit
TTAATGGGGGGCAGCTGCACTTTTAGGTTGCCTATAGGAAATATTGCATATG1300.11909887955438696No Hit
CATCGTGGGAACCAATTCTTGTCATGGTGAAATCTGATTGAGTAACCAAGCA1290.11818273432704551No Hit
TTCTACGGGCTCTTCCGATCTACAAAAGGGATCCTTGAATTGCGTCCACAAG1290.11818273432704551No Hit
GTGCACGGGTAACTGGCAGTGCCACAATTGAACCTAGGTCTGTTTTTCTGTC1270.11635044387236265No Hit
CTGGATGGGGACGCTCTTCCGATCTATGTAGGGGAAGAGATATTCAGCATTT1270.11635044387236265No Hit
TTATGCAGGGCAAGGAGGTGAAAGATCTCTACAAGGAAAACTATGAAACACT1260.11543429864502121No Hit
TTTGTTGGGCTTCAAAAGGACAGGTGATTGTCTCCTGAAGCTGAATTTTAGT1260.11543429864502121No Hit
TGTGCTGGGATGCTTGAAGTATTTGGTGAAAAGATGTTGGTTGACCATAATT1260.11543429864502121No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1250.11451815341767978No Hit
CTGTCAGGGATCTTCAAGCACAGAGGAAAGCAAAGATCCTTCATCAGAAAAG1230.11268586296299689No Hit
AAAAGCCGGGATCTTGAGCCCAAGCAATTTGAAGGTGTTTAAGCTAATTCTT1230.11268586296299689No Hit
GCAACAGGGAACCAGAGTAATGACCTCAGTGGATTTGCTTTAACCCTCACAT1230.11268586296299689No Hit
TATCAAGGGCAGGAGAGCCACAGCCATGATACAGGGCTCTTATGGAGCCCTG1210.11085357250831401No Hit
TGAACTGGGAGAGAGAAGGAATGGCGTGGGATGGGGGAAAGAAAAGAAAGAG1210.11085357250831401No Hit
ACTATAGGGATAGTACCTGAGCTGGTCATTTAAAACGGGTATGATTTTGATC1210.11085357250831401No Hit
GCAGTTGGGATGTGTTAAGATTAATTCATGTTATATGTTCATGAGAAAAATG1200.10993742728097257No Hit
ACTGCAGGGAGAGCGCCCTCATTTGTGGGGCTGCCCCTCCGGGTTTCTCATC1190.10902128205363114No Hit
AATCTTGGGATGAGTGAACTTTATGGTATCTAAATTATACCTAAATCAAGCT1180.1081051368262897No Hit
GAACAGGGGGAAGCGACAGACATATCTATTTCACATTTCACTAGAAATGGCA1180.1081051368262897No Hit
TCAAACGGGAGTATTCAATGATTTTTTATGATCAAGGTGAAATAAACAGTCT1180.1081051368262897No Hit
TGCCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1180.1081051368262897No Hit
GGTGTGGGGAGAAAAAATAGTAAATAATGTTCAAAATGAGAAGACAATAACT1170.10718899159894828No Hit
ATAAGGGGATTATTAGTGATGTTGAACATTTTTTCATATATCTGCTAGCCGT1170.10718899159894828No Hit
CGTGATGGGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT1170.10718899159894828No Hit
GAAACAGGGCAAAAATTAACATGGGCACAGTTGTATTAACTAAACTCCCGAC1160.10627284637160683No Hit
ACTCAGGGGTATGTATGGAAGATATAAAGATTTTTTTGCATTTAGCATCCAC1150.10535670114426539No Hit
GGCACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1140.10444055591692396No Hit
CAAATCGGGATAAGCCTTTTGACTTTTCTAGATGTGAAACCATGAAAGGGCA1140.10444055591692396No Hit
TTGCGCGGATAGAAGAATGGGGGAAGAAAGGATAGAATATTAAAACTGCTTT1140.10444055591692396No Hit
TATAACGGGAGTTCCTACTGGAAAAAGAGTGGAAATTTATTAAAATCAGAAA1140.10444055591692396No Hit
TATCAAGGGGTTACTGCCTATATCTAGGCTGAATGTAAAATCCCTTATGTTG1130.10352441068958251No Hit
GATAGCGGGTTCTCAGACTATCTGATGGGGTTTATTAACTCTGGGATCTTAA1130.10352441068958251No Hit
GCGAGACGGGATCCACACTGTACTGTGATATAGGTACTCTGATTTAAAACTT1120.10260826546224107No Hit
AATACAGGGACAGCCTCTTCCTGTATTGGGAGTGGCACTTGGCCAGGGCATT1120.10260826546224107No Hit
GTCCAAGGGAGCAAACAAAAATAGTGAAGAGTAAACAAAAAAACACAAAAAT1120.10260826546224107No Hit
GGTGCTGGGCACTGCTGGCCTTGCTGCAGAAGTGGCTGCTAGATCTCTCTCC1110.10169212023489964No Hit
GCTGAAGGGATTCCTTCAAGCCTTCTTTATTATGTTTCAACCTTTGTAATGT1100.1007759750075582No Hit
TTTAACGGGGTGCTGATGCTGGTCTAGTTGCTGGCAACATAACCATGAACAA1100.1007759750075582No Hit
GTAGGGGGGGGAAAATTAATCTTCGAGAAATCTTTTCTGACAGCGGTTAGTG1100.1007759750075582No Hit
GAATATGGGCGATCTGCATACGGGAAGCTCAATCTACAGATTTGCCTGTTAT1100.1007759750075582No Hit
GCGGATGGGGGACCAGGCAACAGGCATAGTTAATAGCTGAATTCCTAAGATC1100.1007759750075582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)