FastQCFastQC Report
Sat 31 Dec 2022
SRR5846376.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846376.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108628
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC26492.438597783260301No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT23652.1771550613101596No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT4490.41333726111131575No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3750.3452148617299407No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2170.199764333321059No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1980.1822734469934087No Hit
GTGCTGGGATATTATGATCTTGCTGCAGCCACAGTGCAGCTCCACATTAACT1940.178591155134956No Hit
GACGCAGGGCGATCTTTAAACGGGAGATTTTCCAATTTATTCACATATAGTT1930.17767058217034282No Hit
TTGATCCGGGAATCCACAGATTCACCACTTCATAAGTGGATAATCGAATTGT1720.15833854991346613No Hit
ATGACCGGGCTCCTGCAGCATATTGAACCAATCCACAGAGTGGATATGTCCA1700.15649740398423978No Hit
AAGAACGGGAATGATTTTGAAGGAAAGCTGATAACTATTTTGAGTTAAATTT1560.14360938247965532No Hit
TTCCAGGGCACACCATGAATGTGCCCAAGGTAGTTAGGATTCTGAGAGCGTA1550.14268880951504218No Hit
GTCCAAGGGAGCAAACAAAAATAGTGAAGAGTAAACAAAAAAACACAAAAAT1520.13992709062120265No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1510.13900651765658945No Hit
AAGAGAGGGCCAAGAAACGGTGAGGATAGAAGGGGCCGTAAAATATCAAACA1490.13716537172736312No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1480.13624479876274995No Hit
TCCTTGGGGAATTGTTGATTGATTGAGAGTTATGATCTTCTTGTATTCTTTT1480.13624479876274995No Hit
TGTGCTGGGATGCTTGAAGTATTTGGTGAAAAGATGTTGGTTGACCATAATT1440.13256250690429722No Hit
AATCTTGGGATGAGTGAACTTTATGGTATCTAAATTATACCTAAATCAAGCT1430.13164193393968407No Hit
GCAGTTGGGATGTGTTAAGATTAATTCATGTTATATGTTCATGAGAAAAATG1430.13164193393968407No Hit
CCTTTAGGGCTCATGGCAGTCAAGCAGAAGCAGCCCAGTTTCTCCCAGCAGA1430.13164193393968407No Hit
GTGCACGGGTAACTGGCAGTGCCACAATTGAACCTAGGTCTGTTTTTCTGTC1410.1298007880104577No Hit
TAACTAGGGACCATCCAAGCTGTCTAACCTAGAAGGTAGTAGAAATGGACTC1370.12611849615200502No Hit
CATGTAGGGATGGCAATAGTAATGCCTTACCTATTGATAATTACTTTGTGTA1350.12427735022277867No Hit
TGCTGGCGGGACTGTTTTCAGAGATGAAATAGGAACATAAGCAGGAAGATTT1340.12335677725816548No Hit
GATAGCGGGTTCTCAGACTATCTGATGGGGTTTATTAACTCTGGGATCTTAA1340.12335677725816548No Hit
TTCTACGGGCTCTTCCGATCTACAAAAGGGATCCTTGAATTGCGTCCACAAG1330.12243620429355231No Hit
TATTATGGGATGGGAAAACGTGTTAGCAGTAACATCTCAGAAGACCCTGTAC1330.12243620429355231No Hit
TCAATAGGGGGAAAACAGCGTGGAGATTCCTTAAAGAACTAAAAGTAGAACT1310.12059505836432595No Hit
CAAATCGGGATAAGCCTTTTGACTTTTCTAGATGTGAAACCATGAAAGGGCA1310.12059505836432595No Hit
TGCGTGGGGCATCTTTGTTGGATAAACAAAAATTTGAGGCTATGTAAATGAT1310.12059505836432595No Hit
GCAACAGGGAACCAGAGTAATGACCTCAGTGGATTTGCTTTAACCCTCACAT1300.11967448539971279No Hit
GAAACAGGGCAAAAATTAACATGGGCACAGTTGTATTAACTAAACTCCCGAC1290.11875391243509961No Hit
ATTCTAGGGAAGAACCTTTAGATATAAATGCATTTTAACTGTTCTGGAGAGT1290.11875391243509961No Hit
ACTGCAGGGAGAGCGCCCTCATTTGTGGGGCTGCCCCTCCGGGTTTCTCATC1280.11783333947048642No Hit
GGCGCAGGGATGGGAGCGTTTCGCTCTCCTTGTAGGCTGAAGTCAGTCTGAC1280.11783333947048642No Hit
TTATGCAGGGCAAGGAGGTGAAAGATCTCTACAAGGAAAACTATGAAACACT1270.11691276650587325No Hit
GATATAGGGGATCTTTGCGAGGGACCTATGGGTCTTTTCAATGACCATAAAA1270.11691276650587325No Hit
TCAATGGGGGACGCTCTTCCGATCTTTGTAAGGGGAGGATGAAAATCCTTCT1260.11599219354126009No Hit
GAACAGGGGGAAGCGACAGACATATCTATTTCACATTTCACTAGAAATGGCA1260.11599219354126009No Hit
CTAGTTGGGGAGTGCAATGGTGCAATGATCTCGGCTCACTGCAACCTCTGCC1260.11599219354126009No Hit
CTTGTCGGGAGGAATGCCAAAGTAACATTTAATGTACTTCTCTATAACTTTT1260.11599219354126009No Hit
TGACGCGGGACACACATGAGTGCAGGAGGGCAGACACAACACTCAGTTTTGT1260.11599219354126009No Hit
TGAACTGGGAGAGAGAAGGAATGGCGTGGGATGGGGGAAAGAAAAGAAAGAG1250.1150716205766469No Hit
ATTTCAGGGATTTGAACAATAGTGTTTGCCATGGTTTGAATGATGGTATCTG1250.1150716205766469No Hit
AATTTGGGGATTATGTCCAACTTCAATTGGGTCTTCTGATCTATTAGATTCT1250.1150716205766469No Hit
GTAGCGGGGCCAAGGAGTAGTGAGTTGTAGATAAAAAAAGAATGTCAGCTTT1250.1150716205766469No Hit
TATACTGGATGACAGATGTATAATAAAAGCTTTTTCCCTAAACTGTTTAAGG1250.1150716205766469No Hit
TGCCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1240.11415104761203372No Hit
ACAATGGGGCCTTACCAAGCAACTACTTCACTGTATGGCCTGCAACCAACTT1240.11415104761203372No Hit
CATCGTGGGAACCAATTCTTGTCATGGTGAAATCTGATTGAGTAACCAAGCA1230.11323047464742056No Hit
GTTCCTGGGATAGCGCACAGCGCGCCTTAGCAGCAGCAGCAGCAGCAGCAGC1210.1113893287181942No Hit
ACAAATGGGTGGGTAGGGGGAAGAGGGAGTTGCCGCTGGCCTCAGGGGAATT1210.1113893287181942No Hit
CCAGGTGGGTGAGTTCAAGCCGGATGTGCTCCTGTATTTAGCATGACAGGAA1200.11046875575358103No Hit
TCTATAGGGAAGAAACTTGAAGATTTAAAATAATTTGTTATGACTTTGGTGT1200.11046875575358103No Hit
GCACAAGGGGATTAATGTGAAAAATAATTCCTATGGACACTCCGTTTGAAGT1200.11046875575358103No Hit
AACAAAGGGCATGCCCTTCCCTTTCCCTACCCCTGATAAGATCAAGTTGTTT1190.10954818278896786No Hit
CTGTCAGGGATCTTCAAGCACAGAGGAAAGCAAAGATCCTTCATCAGAAAAG1180.10862760982435467No Hit
TTAGAAGGGAGGGACTGGCAAGACTGAAAATCAGAGCATATAACCATATAAG1180.10862760982435467No Hit
ACCTGTGGGTCTTTGCATCCCTTTAAAATATTTAATTTTTTCTGGCTTTCTC1180.10862760982435467No Hit
CTGGATGGGGACGCTCTTCCGATCTATGTAGGGGAAGAGATATTCAGCATTT1180.10862760982435467No Hit
TATAACGGGAGTTCCTACTGGAAAAAGAGTGGAAATTTATTAAAATCAGAAA1180.10862760982435467No Hit
AAATACGGGAACCTACTTAGTTCCTTTGAAATGAAGTAAAGGTAATAGACAA1170.1077070368597415No Hit
TTGCGCGGATAGAAGAATGGGGGAAGAAAGGATAGAATATTAAAACTGCTTT1170.1077070368597415No Hit
GCGTGCGGGAGTGCTGTCGCGGGAAGTGCAGAGGCAAATGCATTTAGTGTTC1170.1077070368597415No Hit
CGTGATGGGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT1170.1077070368597415No Hit
TAATGTGGGACGCTCTTCTTTTCATTATTTCTAATGCTACAAGAATGCTGTA1170.1077070368597415No Hit
TTTAACGGGGTGCTGATGCTGGTCTAGTTGCTGGCAACATAACCATGAACAA1160.10678646389512833No Hit
TCACATGGGATGCATAGAAGTGGAAAGTAGGGTTTCTCATTTTAAGGTATTT1160.10678646389512833No Hit
CAGTTAGGGATGGTGTTACCTTCCAGCCAAGGGTTGCCTTAAATTGTGCCAG1160.10678646389512833No Hit
TCAAACGGGAGTATTCAATGATTTTTTATGATCAAGGTGAAATAAACAGTCT1150.10586589093051514No Hit
ATAGCAGGGTCACAATGCAATTAAAATGAATGAACTTCAGGTACATCCAACA1150.10586589093051514No Hit
GACGATGGGCAGAGGTGCTGGAGCTGGCAGGTAATGCTTCTAAGGATCTCAA1140.10494531796590198No Hit
TGCATAGGGATGGTAATGAAAATTCATGCATATTGGAACCATGCAAATAAAA1140.10494531796590198No Hit
CATGTGGGCTATAGAGAATCATATGGTCACAGTCAACTTTTTAAAAAAGCTT1140.10494531796590198No Hit
CTTGCTGGGCTCTTGTTATTGATTTCCAGTTTTATTCCATTGTGGTCAGAGA1140.10494531796590198No Hit
GCTATAGGGAATGGGGCAGGCATTAAATAGAAGAATCCCTGCTGTTTAAATT1130.1040247450012888No Hit
ATAAGGGGATTATTAGTGATGTTGAACATTTTTTCATATATCTGCTAGCCGT1130.1040247450012888No Hit
CAAAACGGGAGGTGATTTCAGCACTTCTCTTTAAATAATGGATAGGATAATT1130.1040247450012888No Hit
TATACAGGGATCGTTCCTGTATCCTGGTCTCTATGACTTAGTGTGGTGATGG1120.10310417203667561No Hit
AATACAGGGACAGCCTCTTCCTGTATTGGGAGTGGCACTTGGCCAGGGCATT1120.10310417203667561No Hit
GCTGAAGGGATTCCTTCAAGCCTTCTTTATTATGTTTCAACCTTTGTAATGT1120.10310417203667561No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1120.10310417203667561No Hit
GGTGTGGGGAGAAAAAATAGTAAATAATGTTCAAAATGAGAAGACAATAACT1110.10218359907206245No Hit
TTTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCAGTACATATTTTAT1110.10218359907206245No Hit
ATCTTAGGGCATGTTGTTTCTCCTTTGGATCTACATTTACTAGAGGTGGTAG1100.10126302610744928No Hit
TTAATGGGGGGCAGCTGCACTTTTAGGTTGCCTATAGGAAATATTGCATATG1100.10126302610744928No Hit
CGCACTGGGATGAACTCACTGGTGCTACTTCACTAACAAGATATATTTGAGG1090.10034245314283612No Hit
TATCAAGGGCAGGAGAGCCACAGCCATGATACAGGGCTCTTATGGAGCCCTG1090.10034245314283612No Hit
GTACAAGGGATGAGATCGCCTCTGTCCCAGTCTCCTTACAGACACTGAAACC1090.10034245314283612No Hit
GCTTGAGGGAGCCAGAAAGTGGCAGAGCCAGAATCTGAATGTAAGTCTTTCT1090.10034245314283612No Hit
GGTGCTGGGCACTGCTGGCCTTGCTGCAGAAGTGGCTGCTAGATCTCTCTCC1090.10034245314283612No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)