FastQCFastQC Report
Sat 31 Dec 2022
SRR5846375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109633
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC24602.2438499356945445No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT22572.0586867092937347No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT5090.46427626718232645No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3440.3137741373491558No Hit
GTGCTGGGATATTATGATCTTGCTGCAGCCACAGTGCAGCTCCACATTAACT2170.19793310408362444No Hit
GACGCAGGGCGATCTTTAAACGGGAGATTTTCCAATTTATTCACATATAGTT2060.18789962876141308No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2000.18242682404020688No Hit
TCCTTGGGGAATTGTTGATTGATTGAGAGTTATGATCTTCTTGTATTCTTTT1990.18151468992000583No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1960.17877828755940273No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1900.17330548283819655No Hit
ATGACCGGGCTCCTGCAGCATATTGAACCAATCCACAGAGTGGATATGTCCA1640.14958999571296963No Hit
TTCTACGGGCTCTTCCGATCTACAAAAGGGATCCTTGAATTGCGTCCACAAG1540.1404686545109593No Hit
TTCCAGGGCACACCATGAATGTGCCCAAGGTAGTTAGGATTCTGAGAGCGTA1530.13955652039075825No Hit
AAATACGGGAACCTACTTAGTTCCTTTGAAATGAAGTAAAGGTAATAGACAA1530.13955652039075825No Hit
GAACAGGGGGAAGCGACAGACATATCTATTTCACATTTCACTAGAAATGGCA1510.1377322521503562No Hit
GTGCACGGGTAACTGGCAGTGCCACAATTGAACCTAGGTCTGTTTTTCTGTC1490.13590798390995412No Hit
TCAATAGGGGGAAAACAGCGTGGAGATTCCTTAAAGAACTAAAAGTAGAACT1470.13408371566955207No Hit
TTGATCCGGGAATCCACAGATTCACCACTTCATAAGTGGATAATCGAATTGT1470.13408371566955207No Hit
CCTTTAGGGCTCATGGCAGTCAAGCAGAAGCAGCCCAGTTTCTCCCAGCAGA1450.13225944742914997No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1410.12861091094834584No Hit
CCGTCGGGGATGCTTGCTCTGGCCATGGTTAGTCTGGAAATGGAGAAACTCA1400.12769877682814482No Hit
TATTATGGGATGGGAAAACGTGTTAGCAGTAACATCTCAGAAGACCCTGTAC1390.12678664270794376No Hit
ATTCTAGGGAAGAACCTTTAGATATAAATGCATTTTAACTGTTCTGGAGAGT1390.12678664270794376No Hit
ATTTCAGGGATTTGAACAATAGTGTTTGCCATGGTTTGAATGATGGTATCTG1380.12587450858774274No Hit
ACAATGGGGCCTTACCAAGCAACTACTTCACTGTATGGCCTGCAACCAACTT1370.12496237446754171No Hit
TGTGGACGGGCACTTTCTCTTTAGAAAAAAAGTACTACCTGTTAACAATTGG1370.12496237446754171No Hit
TCACATGGGATGCATAGAAGTGGAAAGTAGGGTTTCTCATTTTAAGGTATTT1370.12496237446754171No Hit
GATAGCGGGTTCTCAGACTATCTGATGGGGTTTATTAACTCTGGGATCTTAA1360.12405024034734066No Hit
CAAATCGGGATAAGCCTTTTGACTTTTCTAGATGTGAAACCATGAAAGGGCA1350.12313810622713962No Hit
TGTGCTGGGATGCTTGAAGTATTTGGTGAAAAGATGTTGGTTGACCATAATT1350.12313810622713962No Hit
TAATGTGGGACGCTCTTCTTTTCATTATTTCTAATGCTACAAGAATGCTGTA1350.12313810622713962No Hit
AAGAGAGGGCCAAGAAACGGTGAGGATAGAAGGGGCCGTAAAATATCAAACA1340.12222597210693861No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1340.12222597210693861No Hit
GTTCCTGGGATAGCGCACAGCGCGCCTTAGCAGCAGCAGCAGCAGCAGCAGC1320.12040170386653654No Hit
TTTGTTGGGCTTCAAAAGGACAGGTGATTGTCTCCTGAAGCTGAATTTTAGT1320.12040170386653654No Hit
GCGGATGGGGGACCAGGCAACAGGCATAGTTAATAGCTGAATTCCTAAGATC1320.12040170386653654No Hit
GAAACAGGGCAAAAATTAACATGGGCACAGTTGTATTAACTAAACTCCCGAC1310.1194895697463355No Hit
ATAAGGGGATTATTAGTGATGTTGAACATTTTTTCATATATCTGCTAGCCGT1310.1194895697463355No Hit
TGAACTGGGAGAGAGAAGGAATGGCGTGGGATGGGGGAAAGAAAAGAAAGAG1310.1194895697463355No Hit
AATCTTGGGATGAGTGAACTTTATGGTATCTAAATTATACCTAAATCAAGCT1300.11857743562613447No Hit
GGTTTTGGGGATGAAAATTAGTTTTTTAAAAAACATCTAGCAGTGCATCTTT1300.11857743562613447No Hit
CTGGATGGGGACGCTCTTCCGATCTATGTAGGGGAAGAGATATTCAGCATTT1300.11857743562613447No Hit
TTTTCTGGGGATTGCATCAGCCAAAAAGGAAAGGCAGGAGGGAATTTAGAGT1300.11857743562613447No Hit
AATTTGGGGATTATGTCCAACTTCAATTGGGTCTTCTGATCTATTAGATTCT1290.11766530150593343No Hit
ACTATAGGGATAGTACCTGAGCTGGTCATTTAAAACGGGTATGATTTTGATC1280.1167531673857324No Hit
CAGTTAGGGATGGTGTTACCTTCCAGCCAAGGGTTGCCTTAAATTGTGCCAG1280.1167531673857324No Hit
TCAATGGGGGACGCTCTTCCGATCTTTGTAAGGGGAGGATGAAAATCCTTCT1270.11584103326553136No Hit
CTGTCAGGGATCTTCAAGCACAGAGGAAAGCAAAGATCCTTCATCAGAAAAG1260.11492889914533035No Hit
TTAATGGGGGGCAGCTGCACTTTTAGGTTGCCTATAGGAAATATTGCATATG1260.11492889914533035No Hit
CATGTGGGCTATAGAGAATCATATGGTCACAGTCAACTTTTTAAAAAAGCTT1260.11492889914533035No Hit
CAAAACGGGAGGTGATTTCAGCACTTCTCTTTAAATAATGGATAGGATAATT1250.11401676502512931No Hit
AAACTTGGGGCATCAGCTGTTTAACTTTGAGCTGAGTGAGGGGAGAGGGGTA1240.11310463090492825No Hit
TTTAACGGGGTGCTGATGCTGGTCTAGTTGCTGGCAACATAACCATGAACAA1240.11310463090492825No Hit
TTTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCAGTACATATTTTAT1240.11310463090492825No Hit
ACCATCGGGTATTTGATACCAAGGGCTTTTTAAATGTCATGGGGGGGAAAAA1240.11310463090492825No Hit
TAACTAGGGACCATCCAAGCTGTCTAACCTAGAAGGTAGTAGAAATGGACTC1240.11310463090492825No Hit
TGCTGGCGGGACTGTTTTCAGAGATGAAATAGGAACATAAGCAGGAAGATTT1230.11219249678472722No Hit
GAATATGGGCGATCTGCATACGGGAAGCTCAATCTACAGATTTGCCTGTTAT1220.11128036266452618No Hit
AAAAGCCGGGATCTTGAGCCCAAGCAATTTGAAGGTGTTTAAGCTAATTCTT1210.11036822854432517No Hit
AACAAAGGGCATGCCCTTCCCTTTCCCTACCCCTGATAAGATCAAGTTGTTT1210.11036822854432517No Hit
GTCCAAGGGAGCAAACAAAAATAGTGAAGAGTAAACAAAAAAACACAAAAAT1210.11036822854432517No Hit
TTCACCGGTATAGATAACATCCTAAGTTAACATATGGCTTCTTAAAACTTGG1210.11036822854432517No Hit
TCTATAGGGAAGAAACTTGAAGATTTAAAATAATTTGTTATGACTTTGGTGT1200.10945609442412413No Hit
TATACTGGATGACAGATGTATAATAAAAGCTTTTTCCCTAAACTGTTTAAGG1200.10945609442412413No Hit
CTGTTTGGGTCCGATCTAAAAAGGGGGTGTATTATAATTTTTAAAAAGGCCC1190.10854396030392309No Hit
TGACGCGGGACACACATGAGTGCAGGAGGGCAGACACAACACTCAGTTTTGT1190.10854396030392309No Hit
TTATGCAGGGCAAGGAGGTGAAAGATCTCTACAAGGAAAACTATGAAACACT1180.10763182618372205No Hit
TGCGTGGGGCATCTTTGTTGGATAAACAAAAATTTGAGGCTATGTAAATGAT1180.10763182618372205No Hit
GATATAGGGGATCTTTGCGAGGGACCTATGGGTCTTTTCAATGACCATAAAA1170.10671969206352103No Hit
CTCGTTGGGATTCCAATAACATGAACTTTCCTTATGCTTTATTCATCATCGC1170.10671969206352103No Hit
AGCAGTGGGAAGCATAGCGTGTTTTCCAGTGTGCCCCAGTCCACCCAGTCTG1160.10580755794331999No Hit
GATAATGGGCTTTTCATGACAAAAGAACTACTCAGTGCTGAAGCAGTGTCAG1160.10580755794331999No Hit
TGCCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1160.10580755794331999No Hit
AATTGGGCAGTCCCAGCCGTGTGCTGGCAGATAGGGTTGTATTATTTCTTCT1160.10580755794331999No Hit
TGCATAGGGATGGTAATGAAAATTCATGCATATTGGAACCATGCAAATAAAA1160.10580755794331999No Hit
CATGTAGGGATGGCAATAGTAATGCCTTACCTATTGATAATTACTTTGTGTA1160.10580755794331999No Hit
TTGCCTGGGCTGCTGGTAAAAGGAGAAACTGAGAGGTGTCCTGCAGACCGGC1150.10489542382311895No Hit
CATAATGGGAGTGCAAAGTCTTTATTCTTCCCATACTTCAACACTGAGTTTT1150.10489542382311895No Hit
GCTCTTGGGAGAATTTCCTAACTATGTTTTCTATTTGTTTATCCACTTGTCC1140.10398328970291791No Hit
AAGAACGGGAATGATTTTGAAGGAAAGCTGATAACTATTTTGAGTTAAATTT1140.10398328970291791No Hit
TTAGAAGGGAGGGACTGGCAAGACTGAAAATCAGAGCATATAACCATATAAG1140.10398328970291791No Hit
AGGCACGGGAGACATGATGGATAGTTGTTTCCCTATAAGATACTGCACCTTG1140.10398328970291791No Hit
GTAGGGGGGGGAAAATTAATCTTCGAGAAATCTTTTCTGACAGCGGTTAGTG1140.10398328970291791No Hit
GGTGCTGGGCACTGCTGGCCTTGCTGCAGAAGTGGCTGCTAGATCTCTCTCC1140.10398328970291791No Hit
GCGTGCGGGAGTGCTGTCGCGGGAAGTGCAGAGGCAAATGCATTTAGTGTTC1130.10307115558271689No Hit
CTTACTCTGGGGAAAAAACTCAGTGTATGATAAAAGTTGCAATTCACAATGT1120.10215902146251585No Hit
AACGGCGGGAACCCTGGTCCCTGGAGTCCCCAGCACCTGGGGCCCTGGTGGG1120.10215902146251585No Hit
TCAAACGGGAGTATTCAATGATTTTTTATGATCAAGGTGAAATAAACAGTCT1120.10215902146251585No Hit
CGTTATGGGAGGTTTCATTTTGCTGTTTTCAGGAATGTAAGAACACCCATAT1120.10215902146251585No Hit
CATCGTGGGAACCAATTCTTGTCATGGTGAAATCTGATTGAGTAACCAAGCA1110.10124688734231481No Hit
GGCACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1110.10124688734231481No Hit
GCTACAGGGTTCTTTTAGGATTGATAAAAGAAGTATACATTTATTTTAATTG1110.10124688734231481No Hit
ATCTTAGGGCATGTTGTTTCTCCTTTGGATCTACATTTACTAGAGGTGGTAG1110.10124688734231481No Hit
CTCAGGGCTATGAGGGCAGGGACTTTCTTTGGTTTTGTCCACTGCTGTGTCC1110.10124688734231481No Hit
AATGACGGGATTTGCAACAACGGAAGCCGCAAAGAAATACTGAATTCGAGGT1100.10033475322211377No Hit
TTGCGCGGATAGAAGAATGGGGGAAGAAAGGATAGAATATTAAAACTGCTTT1100.10033475322211377No Hit
TCAAGGGCACTTTTATTGTGGCTGAATTTTTGTGCCTATCTATAAAACACAC1100.10033475322211377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)