FastQCFastQC Report
Sat 31 Dec 2022
SRR5846370.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846370.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51989
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC21264.0893265883167595No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT18623.581526861451461No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT4930.9482775202446672No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3240.6232087556983208No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1830.3519975379407182No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1790.3443036026851834No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1750.3366096674296486No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC1700.32699224836023005No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1440.2769816691992537No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1350.2596703148743003No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1110.2135067033410914No Hit
GAGCAAGGGAAGACATAAGTCAAAATTTATAATTTTGTAAGACTTCAAACTT1060.20388928427167285No Hit
CATCGAGGGCTGTGACATGTTAGTGATGGGCATGACAGCCATGCTGACAGTT950.18273096231895208No Hit
CAACGCGGGATTTTTATATAATAGCAAATATTTTGGCATATACATTTCAAAT950.18273096231895208No Hit
TTTGTCGGGAGTAGAAAAAAATTAACCATAGATTAAACACCACTCTGGTCAT930.17888399469118466No Hit
TGCAAGGGCTCCTAGATTTGCCAGATTTCAATTTAAAGTTTAAAAAGTGCTA850.16349612418011503No Hit
GCACTGGGGCTCTTTTTTGGTTCCATATGAATTTTAAAGTAGTTTTTTCCAA830.15964915655234763No Hit
CTGAACGGGAAGGCACTGCTCCATCCATTGTTTGGTTCTGAACCTGTTGCTG780.15003173748292908No Hit
TATATGGGGACAGTCGTTTGTTATAATGTTGGGTGTGTTAGACCTCAGTGGC770.14810825366904537No Hit
CTTAGTCTGGGTTCCACTGCAAATGTTTAGATTTCTTTCTTCTTAAGAATAG750.14426128604127797No Hit
CACTAAGGGTCCGCCTTTTCTTCACATCTGTCCCTGGAGGCCGTGAGAAGGA730.14041431841351054No Hit
TAAGTCGGGGACAGTCTCTTTTTACTTGATAAAAGTGGTAAATTTTCTTCAA730.14041431841351054No Hit
ATGACAGGGAGATACTGTGGAGAAGCCATATCTGCAAGTGAGCAGATAAGGC720.13849083459962686No Hit
CTTGTACGGGGCTTCCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAA720.13849083459962686No Hit
TGAACGCGGGGGAAGATTCTTACTGTTAACCAAATTTTGAGCAAGGAGTCTC710.13656735078574314No Hit
GAACGTGGGAGGCACGGTGTCAGCAGGCAACATGGCCGAGAGGCCGGGGCCT710.13656735078574314No Hit
GATGCAGGGGTTGGTCTTACGTATTTCAAGTAAAAAGTAGACAGCTGCACTT700.13464386697185943No Hit
AACTAGGGGAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACA700.13464386697185943No Hit
GTCTGGGGTTTCTGATGTCTTTCTGACCTCACATCATATTTGGTTCTCCTAC690.1327203831579757No Hit
AATCGAGGGGAGCTGGGCTACTGTATACAGTGTACAATGTGTATTTCTTCAA670.1288734155302083No Hit
TAGTGAGGGGGACCAAGGTTGCCTCAGGTAACAAACTACAGAAAGTGAAATT660.1269499317163246No Hit
TTGCCTTATCAGCTTGTGACAACCTTCCCCAGGACAGAAGTCATACAAGGCC660.1269499317163246No Hit
CTCTATGGGATTGATACAAAGTACAGAAAAATACTTTGATTTTTATCCATTT660.1269499317163246No Hit
CATCACGGGAGTGACCTTAGCGAAGCAAGCACATGTGGATGTGAAATTTCAG650.12502644790244088No Hit
ACTACAGGGTATTACCCCCGATCATGCAAAGGCTGTTACCACAATAGTTTAT650.12502644790244088No Hit
TTTGATGGGGAAACGATAAGTAAAAGACAAAATATTTTGTGGTGGGAGCAGT640.12310296408855718No Hit
GTTGCAGGGCATCTCCTCCAATGAGGAAACCCACGCACCTTCCTTAGTAATG640.12310296408855718No Hit
GATGGTGGGCGATTTACTTCTCACAAGGTTATTACCATTCATTTAATTCACA630.1211794802746735No Hit
GATAATCGGGTAGGGATATTATAAGGATTAAATGAAGTATTGCCAAAAATAC620.11925599646078978No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCG620.11925599646078978No Hit
ATGCAGGGGGACCGTAGTGTAGAAAGGGAAAGTCTCTTGTGTGTTCTGCAAT610.11733251264690607No Hit
TGTCTAGGGCATGCACAGAACACTATGCATTTTGAAACTTGTTCATCCTGGA610.11733251264690607No Hit
TTAGGTTGGGGACTGAAGCAAGGAAAAAAAGCAAATCCCCAAACGTGCTAAT610.11733251264690607No Hit
CTAGTAGGGCAGCTATCTAGGCATCCTTCAATCAAGTTGACACCTAATATTA610.11733251264690607No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA600.11540902883302236No Hit
TACTGCGGGATGGGATAGAGAGATTCTTCTTAATATCCTTACCCTATTTGAG600.11540902883302236No Hit
ACAGCAGGGGAACCTGTTAGCTGCTCCTCTCCGCAGCCAGAAATGCTGCCGG600.11540902883302236No Hit
TGGGCAGGGATCCCTCATTTCAATTTCCAATTCCATTAAGGGACCCTCTCTT600.11540902883302236No Hit
TGACCGGGGGGTCTCTCTGCTGTGGCGTTCTCTCTTGAGAGACCCTCTGAAT600.11540902883302236No Hit
GTGGCAGGGCATGAATATTTGTGACATTTCTCCCTCATTGTTAACCTCTCTT590.11348554501913867No Hit
GACTAGGGGGACCACATGAGAGTGGAATCACTGGTGGGGTGGGGTGGAGAAT590.11348554501913867No Hit
GTCACTGGGGAGCATGTTCATCATGTTGTATGATTTATACTAAGCATTAATG590.11348554501913867No Hit
AGATACGGGATAACTTTTACATATCATGATGAAAATTAGCATTAGGGAACGG590.11348554501913867No Hit
ACGTCTGGGGGCACAACACAAGTGCTCTGTACCTACAGTAGTTGTTATTGGG590.11348554501913867No Hit
AGATATGGGCAATATTTTTTAGACAGTTTGATTATTCTTAAAAATTGAAAAC580.11156206120525496No Hit
TCTGAAGGGGCAGAAAACAGTGCCCCAAATGGAAAAAAGATACTCACACAGA580.11156206120525496No Hit
TGGCACGGGTACTCAATATGGAACTACAAAGAATCCAGGTAGAACACAAAAT580.11156206120525496No Hit
ACATTAGGGGAAACAATGTCATCAGGGAAAAGCAAGACAGAATTTGGAATTC580.11156206120525496No Hit
TTATGTGGGAAAGAATTGTGGCTTATAATTTATCTGAAATTTATTAGCTTAG570.10963857739137124No Hit
AGCTTCGGGAAGGTCAGGGACGAGGTTGGAACACATGGATTTTATTCTACAG560.10771509357748754No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC560.10771509357748754No Hit
TAGATAGGGATGAGATAAAATGATGAGATGAGATGAACGATGAGATGAAATG560.10771509357748754No Hit
ACTGCGGGATGATGAGATAAGATGAAAAGGGTTGAGATGATGAGATGAAATG560.10771509357748754No Hit
TAAGTCGGGTCCTTACTGTAATTCTGGCATGGTGATGGAAAAGTGGCATCAC560.10771509357748754No Hit
ATGTTTGGGCATTGATCTAAGTGTGTGCACACAATGGATGAAGAAGAGAAGG550.10579160976360384No Hit
ACTTTGGGGAAAGCTTTATAAGTTCAAGGGCTGTAAAATATGAACTACTTAG550.10579160976360384No Hit
TTACAGGGGCAGGGGACTTAATGACAATTTCTCATCTTCATTGATTGTGTCA550.10579160976360384No Hit
AGCAATGGGATCTTATGAGAAGGCTGCATGTGTGCCTTTAGGATGCAGACAT550.10579160976360384No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATG540.10386812594972014No Hit
ACCATGGGGAGGCAGCAGGCATACAGAAGGTTTGTCCTGCTCTGGGAAGCAT540.10386812594972014No Hit
TGCATGGGGATGGGAAAAAATTTTTTTGATGTTTCTGTGGGTATGCCTTCAC540.10386812594972014No Hit
TAAGTCGGGTATAATAACAGGATGTAAAATATTTTCTTGTCAGGTATTTCTC540.10386812594972014No Hit
AATACAGGGTCGCCGGACCCGGTGGTCGTCCGCGGCTCCGGTCACGTCACCG540.10386812594972014No Hit
TAATGCGGGTATCTACAGACCAGGACTAGAACTGGGCTTTTCTGTCTACTTC530.10194464213583643No Hit
GACAGTGGGGGTTGTGTATTTATCTTAAAATGTTCCTTCTCACTTATTCTAC530.10194464213583643No Hit
CCTGTGGGGCAGCAAGATGGATAAGAACAAAGATGACCAGATTACACTGGAT530.10194464213583643No Hit
ATTCGCGGGTGGGTTATATGAATGGCTACTCTGTCTTGATTCTCAGTACAAA520.10002115832195271No Hit
AGCTGTCGGGGAACATCAGCTCGGATGTTTGTCTCTCAGCCCTGACACCTGC520.10002115832195271No Hit
CGTCATGGGTAAAGTGAAGACGCTGACCGGAAAGGAGATTGAGATTGACATT520.10002115832195271No Hit
AGATATGGGACTGCTCCTTTACGATATTCCAAATAGTTTTTTACATTGGAAA520.10002115832195271No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCT520.10002115832195271No Hit
GTAATAGGGAGCAAATTCTACCAATATTGCATTCTGAGCTAGCAAATGTTCC520.10002115832195271No Hit
TGCTATGGGGAATCAGACAGCTGTGTGCAGCTCTCAGCTACACATTTGATAG520.10002115832195271No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)