FastQCFastQC Report
Sat 31 Dec 2022
SRR5846369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52360
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC19873.794881588999236No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT18183.472116119174943No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT5110.9759358288770054No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3150.6016042780748663No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC1820.34759358288770054No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1810.3456837280366692No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1580.3017570664629488No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1530.2922077922077922No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1440.2750190985485103No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1330.2540106951871658No Hit
TGCAAGGGCTCCTAGATTTGCCAGATTTCAATTTAAAGTTTAAAAAGTGCTA1160.22154316271963329No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1140.21772345301757068No Hit
GAGCAAGGGAAGACATAAGTCAAAATTTATAATTTTGTAAGACTTCAAACTT1120.21390374331550802No Hit
CAACGCGGGATTTTTATATAATAGCAAATATTTTGGCATATACATTTCAAAT910.17379679144385027No Hit
ATGACAGGGAGATACTGTGGAGAAGCCATATCTGCAAGTGAGCAGATAAGGC860.16424751718869365No Hit
TTTGATGGGGAAACGATAAGTAAAAGACAAAATATTTTGTGGTGGGAGCAGT860.16424751718869365No Hit
CATCGAGGGCTGTGACATGTTAGTGATGGGCATGACAGCCATGCTGACAGTT840.16042780748663102No Hit
TTTGTCGGGAGTAGAAAAAAATTAACCATAGATTAAACACCACTCTGGTCAT830.1585179526355997No Hit
CTTAGTCTGGGTTCCACTGCAAATGTTTAGATTTCTTTCTTCTTAAGAATAG810.15469824293353707No Hit
TATATGGGGACAGTCGTTTGTTATAATGTTGGGTGTGTTAGACCTCAGTGGC790.1508785332314744No Hit
TGAACGCGGGGGAAGATTCTTACTGTTAACCAAATTTTGAGCAAGGAGTCTC790.1508785332314744No Hit
GTCTGGGGTTTCTGATGTCTTTCTGACCTCACATCATATTTGGTTCTCCTAC760.14514896867838042No Hit
GCACTGGGGCTCTTTTTTGGTTCCATATGAATTTTAAAGTAGTTTTTTCCAA730.13941940412528647No Hit
GTTGCAGGGCATCTCCTCCAATGAGGAAACCCACGCACCTTCCTTAGTAATG730.13941940412528647No Hit
TGGCACGGGTACTCAATATGGAACTACAAAGAATCCAGGTAGAACACAAAAT730.13941940412528647No Hit
GCTCATGGGCTCTAAGAAAATGCCCTATTTGCAGGGGTATAATCAAGGGTAC700.1336898395721925No Hit
TGCATGGGGATGGGAAAAAATTTTTTTGATGTTTCTGTGGGTATGCCTTCAC700.1336898395721925No Hit
AACTAGGGGAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACA690.1317799847211612No Hit
AATACAGGGTCGCCGGACCCGGTGGTCGTCCGCGGCTCCGGTCACGTCACCG690.1317799847211612No Hit
TTTCGTGGGGGGTATAAGTTGGGCCCTTGGCTTCTTGCCCTGCCTACCAGCC680.12987012987012986No Hit
TAGTGAGGGGGACCAAGGTTGCCTCAGGTAACAAACTACAGAAAGTGAAATT670.12796027501909854No Hit
GGTAAAGGGTCCCAGTGAATTCTTGAAGGAAAATGATACGCAACATAATTAA670.12796027501909854No Hit
AGATACGGGATAACTTTTACATATCATGATGAAAATTAGCATTAGGGAACGG670.12796027501909854No Hit
TAAGTCGGGGACAGTCTCTTTTTACTTGATAAAAGTGGTAAATTTTCTTCAA660.12605042016806722No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC660.12605042016806722No Hit
CTAGTAGGGCAGCTATCTAGGCATCCTTCAATCAAGTTGACACCTAATATTA660.12605042016806722No Hit
GATGGTGGGCGATTTACTTCTCACAAGGTTATTACCATTCATTTAATTCACA650.1241405653170359No Hit
ATTGTAGGGTGGGGCAGGGCAGCAGGCTTTTGTGTTCTCTAAAAATGTTTTA650.1241405653170359No Hit
ACTGCGGGATGATGAGATAAGATGAAAAGGGTTGAGATGATGAGATGAAATG650.1241405653170359No Hit
TTATGTGGGAAAGAATTGTGGCTTATAATTTATCTGAAATTTATTAGCTTAG640.12223071046600457No Hit
CTCAATGGGACATGGACGCTGAACAGTAAATAAAGTGGATAATTTAAATTTA640.12223071046600457No Hit
CTTGTACGGGGCTTCCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAA630.12032085561497327No Hit
CTGAACGGGAAGGCACTGCTCCATCCATTGTTTGGTTCTGAACCTGTTGCTG630.12032085561497327No Hit
CCGGCTGGGGAGTTTATAAAGTATAATTAATTAAACAAAAAATAAGGATGAG630.12032085561497327No Hit
ACGTCTGGGGGCACAACACAAGTGCTCTGTACCTACAGTAGTTGTTATTGGG630.12032085561497327No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCG620.11841100076394194No Hit
TAAAATAGGGGGGAGGTAAAAATTACAGCACAAAGAAAACTAAGCAGGTGCA620.11841100076394194No Hit
TGCTATGGGGAATCAGACAGCTGTGTGCAGCTCTCAGCTACACATTTGATAG620.11841100076394194No Hit
CCCGTGGGGATTGATGAAGGATCATGCACCTAACACATACTAACTTTTTTAA610.11650114591291061No Hit
CATCACGGGAGTGACCTTAGCGAAGCAAGCACATGTGGATGTGAAATTTCAG610.11650114591291061No Hit
AGCAATGGGATCTTATGAGAAGGCTGCATGTGTGCCTTTAGGATGCAGACAT610.11650114591291061No Hit
AACGGGGCTCAGGCTCAAGGTAACAGTGTTCTTGTGGTTTGACCAATTGTTT600.1145912910618793No Hit
TCTAATGGGAACCATGGTCTTAGAGACTCCGGCTTGGTCATGACATTGAGAA600.1145912910618793No Hit
CAATCCGGGGATGGAACATAGATTAGTGGTTGCCAGGGGATAGAGGAGTAAT590.11268143621084797No Hit
TTACAGGGGCAGGGGACTTAATGACAATTTCTCATCTTCATTGATTGTGTCA590.11268143621084797No Hit
ACATTAGGGGAAACAATGTCATCAGGGAAAAGCAAGACAGAATTTGGAATTC590.11268143621084797No Hit
AGATATGGGACTGCTCCTTTACGATATTCCAAATAGTTTTTTACATTGGAAA580.11077158135981664No Hit
ATACTGGGTTCTAAAGCAGTCCTCAGATTCACACAACGTAGTTTGTGACGGA580.11077158135981664No Hit
TGTCTAGGGCATGCACAGAACACTATGCATTTTGAAACTTGTTCATCCTGGA570.10886172650878534No Hit
TTGCCTTATCAGCTTGTGACAACCTTCCCCAGGACAGAAGTCATACAAGGCC570.10886172650878534No Hit
TACTGCGGGATGGGATAGAGAGATTCTTCTTAATATCCTTACCCTATTTGAG570.10886172650878534No Hit
TTTCCAGGGCTGCATACAACAAAAAACACACTAGCCCCTTCCCCAGGGGAGT570.10886172650878534No Hit
CTCTATGGGATTGATACAAAGTACAGAAAAATACTTTGATTTTTATCCATTT570.10886172650878534No Hit
ACTACAGGGTATTACCCCCGATCATGCAAAGGCTGTTACCACAATAGTTTAT570.10886172650878534No Hit
AATCGAGGGGAGCTGGGCTACTGTATACAGTGTACAATGTGTATTTCTTCAA570.10886172650878534No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATG560.10695187165775401No Hit
GTGGCAGGGCATGAATATTTGTGACATTTCTCCCTCATTGTTAACCTCTCTT560.10695187165775401No Hit
CATTTAGGGAACTGTATTAATTTACTATGAAATGGACATCCTTTTAACTAAA560.10695187165775401No Hit
ACATAAGGGAACCCAAGAAACAATTTTGGGTTAGCCATCTAATATAGCAAAT560.10695187165775401No Hit
TAAGATGGGAAGATATTGTATCTCCTTTAATATACTCCTTAAAAATGTATGT560.10695187165775401No Hit
TAAGTCGGGTATAATAACAGGATGTAAAATATTTTCTTGTCAGGTATTTCTC560.10695187165775401No Hit
TTACTTGGGACACCAAGACACAGGCACACAGAGACTTATTCAAAAGATCATA560.10695187165775401No Hit
CTTCTTGAGGGGGGACAGGCACATTGCAGTTGTAGGTGTTCATTTTCAGCAT560.10695187165775401No Hit
ATTCGCGGGTGGGTTATATGAATGGCTACTCTGTCTTGATTCTCAGTACAAA550.10504201680672269No Hit
TTAACCGGGGTGCTGATAGTAACTGAAGAATAAAACCACATAGAGGAAAAAT550.10504201680672269No Hit
TTAGGTTGGGGACTGAAGCAAGGAAAAAAAGCAAATCCCCAAACGTGCTAAT550.10504201680672269No Hit
GTGCCAGGGCATTACTAGGAATGCATTTCTTATTACCATGGCATGTAGTAGA550.10504201680672269No Hit
TGGGCAGGGATCCCTCATTTCAATTTCCAATTCCATTAAGGGACCCTCTCTT550.10504201680672269No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATC550.10504201680672269No Hit
AGCTCAGGGTTATTTCCAGGTTTTGGCTATTATGAGTAAAGCTGCTATGAAT540.10313216195569137No Hit
ATGTTTGGGCATTGATCTAAGTGTGTGCACACAATGGATGAAGAAGAGAAGG540.10313216195569137No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA540.10313216195569137No Hit
GCTTAGGGGTGTCACTTCTACATTATACAGAACTAAAAGGAAATTCTATCTA540.10313216195569137No Hit
TGTGCTGGGATTAAGGCGTGAGCCACCGTGCCCGGCCAATACGTACTTTAAA540.10313216195569137No Hit
TTGTTTGGGGCATGTAAGACTTTTACACTGTCTATTGTTGGAAAACAATGAG540.10313216195569137No Hit
GCTTTGGGGATATACACACACACATATATATATTTAGAAACGTGAGTGTTAA530.10122230710466004No Hit
CACTAAGGGTCCGCCTTTTCTTCACATCTGTCCCTGGAGGCCGTGAGAAGGA530.10122230710466004No Hit
GATGCAGGGGTTGGTCTTACGTATTTCAAGTAAAAAGTAGACAGCTGCACTT530.10122230710466004No Hit
TAAACAGGGAGGAAAACCTGAGGAAAAAAAATTATAGCAATTAACTGGGCAT530.10122230710466004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)