FastQCFastQC Report
Sat 31 Dec 2022
SRR5846366.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846366.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190260
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC30221.5883527804057607No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT29321.541049090717965No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT5640.2964364553768527No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4520.23756964154315147No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2970.15610217596972564No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2750.14453905182382004No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC2300.12088720697992221No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC2090.10984967938610322No Hit
CATTTAGGGGGTGCTGATGGAGACTTACAGCATTGTGATAGGTTATTTACCC1950.10249132765689058No Hit
CAAAACGGGACATTTGGTTAAAATGAAAATCTCTGCTTAATGGAAAAAATAC1910.1003889414485441No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)