FastQCFastQC Report
Sat 31 Dec 2022
SRR5846365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250550
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT30671.224106964677709No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC27221.0864098982239074No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT4850.1935741368988226No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3200.12771901816004788No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2670.10656555577728997No Hit
CCAACAACGTGAAGACGGCCGTGTGCGACATCCCGCCCCGCGGCCTGAAGAT2630.10496906805028937No Hit
AGCTGAGGGAATCCTTTAATATCATCAAATATCATCATTGTTCAAATTTCCA2570.10257433645978846No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)