FastQCFastQC Report
Sat 31 Dec 2022
SRR5846362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173688
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC38052.1907097784533187No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT33171.9097462115978077No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT5930.34141679333056973No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4620.2659941964902584No Hit
TATAAGGGATCCTTCAAGAATTAAGGCAAAATCAAGATCAAAATCTATCATT2710.15602689880705634No Hit
CTTGATGGGAAGGAAAGCTTTTATCTGAATCCCAATTGAGAATCTGAATTCT2470.14220901846990006No Hit
TTCGCGGGGTTCCTGAAATACCTTAAACTCTTCCTTGCCTCAGGAACTTTGC2440.1404817834277555No Hit
CTAGTAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2420.13933029339965916No Hit
TACAAAGGGTATGTGAAATGTATATTTTTATTTTTCTGAAATTGCCCTTAAA2380.13702731334346646No Hit
AATGGTGGGCATGCAGTTGCTGCGAGACAACCTCACCCTCTGGACGAGCGAC2340.13472433328727373No Hit
TTTGCTGGGGGGGTGAGTTTATTGACTACCCAAACTGTATTTTACCAATAAA2300.132421353231081No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2290.13184560821703284No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC2290.13184560821703284No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2240.12896688314679194No Hit
GGAACCCTAGAAGATTGTGGGGATAGGTAATGGATGGATTCAACATGCCTTT2240.12896688314679194No Hit
CCTAGCGGGCCGGACAGCAGCTTCAAGTTGACCTCTTAGAAGCCCGACCCTA2240.12896688314679194No Hit
TGACTTGGGATATCTATGTGTTACATGTCGATAGATAATATATATCTATGTG2230.12839113813274378No Hit
TTGCTTGGGAGTAATCCTCTTTATAAATTAAACCAGGTTTGTTTAATATATA2230.12839113813274378No Hit
TAACGCGGGCAGCCAGTGTCCATCCAAAAGGCCACAAATTGATTCTCCAGCT2230.12839113813274378No Hit
TACCAGGGGGGGTATATGGTTAGTTTATTTGTTTCATTTAAAAAATCTCTTT2180.12551241306250288No Hit
ATTTGTGGGTCCCTTATTTAATAAGCAAAATATGTTTTTGGAATAAGTGGTG2160.12436092303440652No Hit
AATGGCGGGAGAATACGAGATTCAGAATATGAAATTCAACGACAAGCTAAAA2160.12436092303440652No Hit
ACGTATGGGTACGCAATAAAATATTGATGTTTGAAGGCAGTGGTCACCAATT2150.12378517802035835No Hit
ATTAGAGGGTTCTCTGGAATTGAAATACATCCTAAAATTGTTGGTTTGTCAT2150.12378517802035835No Hit
GGATTCGGGAAGGGTGGAAGTTCTAATGTCTGTTAGCTACCCATAAGAATGC2140.12320943300631017No Hit
AAAAATGGGAAAATGTGTATGATATATTTGAAAAGGCTTCCATTAATATAAT2130.122633687992262No Hit
GCACAGGGGGGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGT2120.12205794297821382No Hit
TGGCTTGGGAGACGCCATGGCTGCATTGAATGATATACGGTATCAGAGACTG2120.12205794297821382No Hit
CATACAGGGAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTGTCTCGCT2120.12205794297821382No Hit
GGCCGCGGGGGGGAAAGAGTTATTCCATAACAGAATTATTGCTTCCCATGGC2110.12148219796416562No Hit
AGCAACGGGGGTGTTTTTAAGGGGGTCTAAGCCTGCCTTTTCAATGATTTAA2110.12148219796416562No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC2110.12148219796416562No Hit
TAATATGGGGGATACAGTTAAAACTGTGGATACAGTTAAAACTGATTAGAGG2090.12033070793606927No Hit
GCACACGGGAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCACA2090.12033070793606927No Hit
TCACCTAGGGGTCTGAAGAATGATTTGATCTCAGAAAATGTTGGCAAAACCA2070.11917921790797292No Hit
TTGAGCGGGGTATACTAACTCAACAGAATAAACTCATAACCACTAGCCTATC2060.11860347289392474No Hit
CACAATGGGGTATGATTTTTAAAGAAAAATCAGGAATCAGCTTATAAGAAAA2060.11860347289392474No Hit
GAACTTGGGATCTGTTCCGCTTCTCAGCGGTCCATCTCAGCGTCTCCCTTCA2050.11802772787987655No Hit
GGACTTGGGGGGCATCCGGGACGGCGGGCGGGCTGGCCACCACGGGACAGGA2040.11745198286582839No Hit
CTCATTGGGCGATCTCTGGCGGGGAGTCCTCGAAGGCTCGGAAGCCCGTCAC2030.1168762378517802No Hit
TGATAAGGGGCATTTCTCAAAGGATGGCTACCATTTTGCTGGAGCCAAAGAA2030.1168762378517802No Hit
CTTATTGGGAAATGACAACTTTGGTGCTTTAAAATGAGGTACCACTTTTTAA2030.1168762378517802No Hit
AAGAATGGGAGGCTTTGGAAGAAAGAATACATGTGTGGTGACCTCTTTGAAA2030.1168762378517802No Hit
TCACACGGGGAATTCCCATTAATGAGCAGTCTTCAGTATTAAAACCACTGTC2020.11630049283773201No Hit
TGCAATGGGCACTGAATTCACTGAGATTATTCCTATAATTTTAAAGTATCAT2020.11630049283773201No Hit
TAACACGGGCAACACTCATTTTGTTGACATGTATCTGACCATGCTGATGTCT2020.11630049283773201No Hit
ATTTAAGGGACGAGACCCTGTCTCCAAAGGAAAAACAAAAAAGAAGAATAAA2010.11572474782368385No Hit
AGTTGGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2000.11514900280963566No Hit
ACTGTCGGGGATTTTCCGACCCGGGCAAGATGGCAGCGGCGCTGCGCGTGCG2000.11514900280963566No Hit
CGTACAGGGGATAGCCATGGCAGCTCAGATGGCAGCCCTGCAAGCTAAAGCT2000.11514900280963566No Hit
CATACGGGGACCTGACTGGAGCCCAGAACTTGGGACCAATACTTAATTCAAA1980.11399751278153931No Hit
ACCTTAGGGTGGATTTTAATGCTTTTATGCATTTTATCTCTGCTTTGATAAA1980.11399751278153931No Hit
ATGCTTGGGGGGTGAAGGGCGGCGCGCTTAGGCAGGCGGTGGCGCGGCTGGA1980.11399751278153931No Hit
ACAGTTGGGAAATGACTAAATGCCAAGTCACTTAAATAGAATAGCAATAGAT1980.11399751278153931No Hit
TTGTTTGGGCCATCAGAGAATCCATAAAGGAGAGAAGCCCTACGAATGCCTC1970.11342176776749113No Hit
CAGATGGGGGGTTTAGAAGGCATATTGAGTTATTTTGCAGAATCATAATGGA1960.11284602275344296No Hit
CATCTAGGGACAGGAACGACATTCATACAGGGACATTTGTGAAAGCAAAGCA1960.11284602275344296No Hit
CACTCGGGGCTGAGGGAACTGCACATTCATCCAATACCTCAGATGTGGATTT1960.11284602275344296No Hit
TAAACCGGGAATAGACCACGATGTTACATCCGCAAAGGATGGTGATTACTGG1940.11169453272534659No Hit
ACAAAGGGATCTGGACATTTGGTGATTTCTGGTTTCTGTTTTTTTGAGTTTT1940.11169453272534659No Hit
AGGTTCGGGGGAAAAGCCTGCATTTCATCAGCAGAAGAATGGATAAATAAAT1940.11169453272534659No Hit
CGATTTGGGCCAAGAAAATGTTTGCCATTTTTGCATTGTTTCGTTTTTAACT1940.11169453272534659No Hit
AAAACCGGGGGGCTACAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGA1930.11111878771129843No Hit
TAAAAAGGGTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAAT1930.11111878771129843No Hit
GCAATTTCCCAGAAGCATTTGTCTCTAACCCATGTGGGTAGCCCGACCATGA1920.11054304269725024No Hit
ATCCCGGGAGTCACTGGTGACCTTGATAAAAGCAACTTCAGTGAAATGGTGA1920.11054304269725024No Hit
AACAATGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1900.10939155266915389No Hit
CCAACTGGGGCCCAAGCGGAGCTGCCCCTTCGCGGACGTGGCCCCGCTACAG1900.10939155266915389No Hit
GTTAACGGGCTTGCCTCCCAGTGTTTCCAGCTCTCCAGCCTCTTTACCCCCC1900.10939155266915389No Hit
TATACGGGGGTGAGAATATAGCCTCCCACCCCATTAACTTCGCTCCCATGGG1880.10824006264105754No Hit
TTGTCAGGGCAAACATCATCTTTATGTTTAGTCAACTGTTTTCATGGGAAAA1870.10766431762700936No Hit
GCATAGGGGAGAAAATTGTATTTTAAATCTTCTAACAGAAAAAGAAGTAAAT1870.10766431762700936No Hit
CGTTAAGGGCAGGGCAGAGAGGTCTGTGGTTCTCAGCATCAGAAGGCAGCGC1860.10708857261296116No Hit
CTTCACCGGGAAGACAAGACAAACTGGGAGAAGATATTTGCCATGCATACAA1860.10708857261296116No Hit
TGCGATTGGGCAGATATGATTTTCCTTTTTCGCATATATGGTTTAATAAATG1850.106512827598913No Hit
TCTCAAGGGGATCTGCTTATGGGTCTTTCAAAAAATGTCATACTCAATTTAA1850.106512827598913No Hit
AAGATAGGGATATGGAATAAAATAAGTATTGAAGAATTTTTCTTTGTAACAA1850.106512827598913No Hit
GATTATGGGAGACAGGATCAATTGTAAAGTGAGGGCTTCTCCATGACACCAT1850.106512827598913No Hit
CTGCCGGGGGTCTTGAACTCCTGGCCTCCAGTGATCTGCCCACGTCGGTCTC1850.106512827598913No Hit
TAGTCAGGGAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACA1850.106512827598913No Hit
CATCACGGGCTTTTTCTCCTCTATGAATAATTTTATATTTCATGCTACTTCT1850.106512827598913No Hit
GCTAATGGGAGATGTATTTCCTGAAGCATATTTTTCATAGAATGTAGCGTGT1840.10593708258486481No Hit
TTCCAGGGTATAGCAAATAGTTGAAACAGAAGTTTTGATTGGCAAATCAGGA1840.10593708258486481No Hit
AGCTAAGGGACTCTGGCCAACTGTTACCAGTTGGTATTTCTACAAGTTCAGA1840.10593708258486481No Hit
TCACTGTGGGCAATGAATAGTTTTAGAGACACAAGCTTAGAATCCCAAAAGG1820.10478559255676846No Hit
GCTGTGTCATTGCATGAGCAACTTTGTGTCTGTGCTTTTTCTACAATTTGGA1820.10478559255676846No Hit
TGGCCGGGGATGGAAACAAGAAATACAGAAAATAAGTCAATCTGAAAGATTT1820.10478559255676846No Hit
AAACCAGGGGAAAGTATCAATTGCTTTAAAACTGTTGTAAGTACAGTTGGCA1820.10478559255676846No Hit
AGAAGTGGGCGATCTCCTGACCTCGTGATCCGCCCGCCTCAGCCTCCCAAAG1820.10478559255676846No Hit
TCAGATGGGCTACAGTGGAGCCAGTCAACATGTGTACAACTTAACACCTAAC1810.10420984754272028No Hit
CAAATCGGGCATTCAGAGATATTTTAAGGACCTTGTTTTAACTTTGTTTCAA1810.10420984754272028No Hit
GGACACGGGGAATATGTCATAACCAATAATCCATGGGTGAGACTAGCTACAG1810.10420984754272028No Hit
TGTTTAGGAACTCAAAATCTTTTTTCCTAGTTTGCTATAAAGTTGCACAAAG1800.1036341025286721No Hit
TTACAAGGGCAATCAAGCACAGGTTTCAAGTCTGGGTTCTGGTGTCCACTCA1800.1036341025286721No Hit
GTCCAAGGGAGGTCAAGAACGTGGCCGATTACTACCCGGAGTACAAGCTGCT1790.10305835751462393No Hit
GACTACGGGTACACTGCGAATTACTACCATCTATTCTCACTGCCAATGGAAA1790.10305835751462393No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1790.10305835751462393No Hit
GAACAGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1790.10305835751462393No Hit
ATAGTGGGGAGAACTTTAGACCCTGTAGCCATAGAGGGAATGGCGTTCCTTT1790.10305835751462393No Hit
TGCAGTGGGTGAACCTCTGTATGTACATCAATGGCCACTCATTTATCTAATG1790.10305835751462393No Hit
AATAATGGGGCTGCCCCATATTTTAGAAAATGTAAAAATGGTGGTTTGGCCA1790.10305835751462393No Hit
TTCAATGGGTAACTAACCACTGGCTCTTTCTCGAAAATTTGGTAGCTGCCCC1780.10248261250057575No Hit
AGTAATGGGAAGGCTGAAAGGAACATTCATACTGGAAGTAGTAGCAGTAGCA1780.10248261250057575No Hit
GTATGAGGGGGAGGAAAACTAAGCATTGGCCTCATGTTCAGTCTTCAGGATA1780.10248261250057575No Hit
ATGCTTGGGCTGAAAGAAAGAGATGTAGAAGAATTACAGCTAAAACTTACAA1770.10190686748652755No Hit
TTGCCGGGCCACCTCCTGGATTCAAGCTATTCTCCTGCCTCAGCCTCTCAAG1760.1013311224724794No Hit
TAATGCGGGATGTTGGGATCACGACGCAGAAGCCAGGTTATCAGCTGGATGT1760.1013311224724794No Hit
TTCTCAGGGGACTCGGGATGCATACATTGTATCTTAATGCTGTTTCAGATCA1750.1007553774584312No Hit
TTATACGGGTAGAGTCTGAAGATTATGCTTTGAGAAATCTTATTTTGGAAGA1740.10017963244438305No Hit
TCGATTGGGGATGAATGGAAAAATACTTTTCAGGGAGAATTGAAGGGGTTAA1740.10017963244438305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)