FastQCFastQC Report
Sat 31 Dec 2022
SRR5846361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174082
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC38252.197240380969888No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT32471.865212945623327No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT5310.3050286646522903No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4660.2676899392240438No Hit
CTTGATGGGAAGGAAAGCTTTTATCTGAATCCCAATTGAGAATCTGAATTCT2710.15567376293930446No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2560.14705713399432452No Hit
CGTACAGGGGATAGCCATGGCAGCTCAGATGGCAGCCCTGCAAGCTAAAGCT2540.14590825013499387No Hit
TACAAAGGGTATGTGAAATGTATATTTTTATTTTTCTGAAATTGCCCTTAAA2500.14361048241633254No Hit
TTCGCGGGGTTCCTGAAATACCTTAAACTCTTCCTTGCCTCAGGAACTTTGC2470.14188715662733653No Hit
CTAGTAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2440.14016383083834055No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2420.1390149469790099No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC2410.13844050504934455No Hit
CTTATTGGGAAATGACAACTTTGGTGCTTTAAAATGAGGTACCACTTTTTAA2390.1372916211900139No Hit
CGATTTGGGCCAAGAAAATGTTTGCCATTTTTGCATTGTTTCGTTTTTAACT2350.1349938534713526No Hit
TATAAGGGATCCTTCAAGAATTAAGGCAAAATCAAGATCAAAATCTATCATT2300.1321216438230259No Hit
ATTAGAGGGTTCTCTGGAATTGAAATACATCCTAAAATTGTTGGTTTGTCAT2300.1321216438230259No Hit
TGATAAGGGGCATTTCTCAAAGGATGGCTACCATTTTGCTGGAGCCAAAGAA2290.1315472018933606No Hit
TTGAGCGGGGTATACTAACTCAACAGAATAAACTCATAACCACTAGCCTATC2280.13097275996369526No Hit
TGACTTGGGATATCTATGTGTTACATGTCGATAGATAATATATATCTATGTG2260.1298238761043646No Hit
AATGGTGGGCATGCAGTTGCTGCGAGACAACCTCACCCTCTGGACGAGCGAC2260.1298238761043646No Hit
TCACCTAGGGGTCTGAAGAATGATTTGATCTCAGAAAATGTTGGCAAAACCA2240.12867499224503395No Hit
TTGCTTGGGAGTAATCCTCTTTATAAATTAAACCAGGTTTGTTTAATATATA2220.12752610838570327No Hit
TTTGCTGGGGGGGTGAGTTTATTGACTACCCAAACTGTATTTTACCAATAAA2220.12752610838570327No Hit
ACTGTCGGGGATTTTCCGACCCGGGCAAGATGGCAGCGGCGCTGCGCGTGCG2200.12637722452637262No Hit
ATGGTTGGGTCCACTTCCTCACCAACATTTTATGTTTTTAGTCTCTTTAATA2170.12465389873737664No Hit
ATGCTTGGGGGGTGAAGGGCGGCGCGCTTAGGCAGGCGGTGGCGCGGCTGGA2170.12465389873737664No Hit
GCTAATGGGAGATGTATTTCCTGAAGCATATTTTTCATAGAATGTAGCGTGT2150.123505014878046No Hit
TAATATGGGGGATACAGTTAAAACTGTGGATACAGTTAAAACTGATTAGAGG2140.12293057294838064No Hit
ATCCCGGGAGTCACTGGTGACCTTGATAAAAGCAACTTCAGTGAAATGGTGA2140.12293057294838064No Hit
CTTCACCGGGAAGACAAGACAAACTGGGAGAAGATATTTGCCATGCATACAA2140.12293057294838064No Hit
TAACACGGGCAACACTCATTTTGTTGACATGTATCTGACCATGCTGATGTCT2140.12293057294838064No Hit
CCTAGCGGGCCGGACAGCAGCTTCAAGTTGACCTCTTAGAAGCCCGACCCTA2140.12293057294838064No Hit
CGTTAAGGGCAGGGCAGAGAGGTCTGTGGTTCTCAGCATCAGAAGGCAGCGC2130.12235613101871533No Hit
AAGATAGGGATATGGAATAAAATAAGTATTGAAGAATTTTTCTTTGTAACAA2130.12235613101871533No Hit
GCACAGGGGGGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGT2120.12178168908904999No Hit
ACAAAGGGATCTGGACATTTGGTGATTTCTGGTTTCTGTTTTTTTGAGTTTT2100.12063280522971932No Hit
CAGATGGGGGGTTTAGAAGGCATATTGAGTTATTTTGCAGAATCATAATGGA2100.12063280522971932No Hit
TTGTGAGGGTAGAAATGAGCAGATTGGTTAAACTACTCTGTTTTCTTAAACT2100.12063280522971932No Hit
ACCTTAGGGTGGATTTTAATGCTTTTATGCATTTTATCTCTGCTTTGATAAA2090.12005836330005401No Hit
GGATTCGGGAAGGGTGGAAGTTCTAATGTCTGTTAGCTACCCATAAGAATGC2080.11948392137038867No Hit
ATTTAAGGGACGAGACCCTGTCTCCAAAGGAAAAACAAAAAAGAAGAATAAA2080.11948392137038867No Hit
CCAACTGGGGCCCAAGCGGAGCTGCCCCTTCGCGGACGTGGCCCCGCTACAG2070.11890947944072333No Hit
TTGCCGGGCCACCTCCTGGATTCAAGCTATTCTCCTGCCTCAGCCTCTCAAG2060.118335037511058No Hit
CATACGGGGACCTGACTGGAGCCCAGAACTTGGGACCAATACTTAATTCAAA2060.118335037511058No Hit
CTGTTAGGGGTGCAGGGCGGGTGGAGTCGCGGAGTAGTCCTCATGGCCGCCC2060.118335037511058No Hit
TTGTCAGGGCAAACATCATCTTTATGTTTAGTCAACTGTTTTCATGGGAAAA2050.11776059558139267No Hit
AAAAATGGGAAAATGTGTATGATATATTTGAAAAGGCTTCCATTAATATAAT2040.11718615365172735No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC2030.11661171172206201No Hit
AGGTTCGGGGGAAAAGCCTGCATTTCATCAGCAGAAGAATGGATAAATAAAT2030.11661171172206201No Hit
TAGTCAGGGAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACA2030.11661171172206201No Hit
AGTAATGGGAAGGCTGAAAGGAACATTCATACTGGAAGTAGTAGCAGTAGCA2020.11603726979239669No Hit
TACCAGGGGGGGTATATGGTTAGTTTATTTGTTTCATTTAAAAAATCTCTTT2020.11603726979239669No Hit
CTTGCTGGGATGTCTTCTTGGAGAATGGACCCCTTTCATCATTATCCCTGAT2020.11603726979239669No Hit
TGGCCGGGGATGGAAACAAGAAATACAGAAAATAAGTCAATCTGAAAGATTT2020.11603726979239669No Hit
CTGCCGGGGGTCTTGAACTCCTGGCCTCCAGTGATCTGCCCACGTCGGTCTC2010.11546282786273135No Hit
AGCTAAGGGACTCTGGCCAACTGTTACCAGTTGGTATTTCTACAAGTTCAGA2010.11546282786273135No Hit
GAACAGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1990.11431394400340068No Hit
CTCATTGGGCGATCTCTGGCGGGGAGTCCTCGAAGGCTCGGAAGCCCGTCAC1980.11373950207373537No Hit
AATTGCGGGAGTATACTCAACGCTAGTACAGTTGCTGCAGCAATGTGCATGG1980.11373950207373537No Hit
GCTCATGGGGTCCTGTGAGCATTCAGTCAGTTGAAATTAAAGATTCCTCATT1980.11373950207373537No Hit
TGGCTTGGGAGACGCCATGGCTGCATTGAATGATATACGGTATCAGAGACTG1980.11373950207373537No Hit
AATGGCGGGAGAATACGAGATTCAGAATATGAAATTCAACGACAAGCTAAAA1970.11316506014407003No Hit
GTTTTTGGGCATCAAGCTACCAATGACTTTCTTCACAGAATTGGAAAAAACT1960.1125906182144047No Hit
AGCAACGGGGGTGTTTTTAAGGGGGTCTAAGCCTGCCTTTTCAATGATTTAA1960.1125906182144047No Hit
TTATACGGGTAGAGTCTGAAGATTATGCTTTGAGAAATCTTATTTTGGAAGA1960.1125906182144047No Hit
GGACACGGGGAATATGTCATAACCAATAATCCATGGGTGAGACTAGCTACAG1960.1125906182144047No Hit
TTACAAGGGCAATCAAGCACAGGTTTCAAGTCTGGGTTCTGGTGTCCACTCA1960.1125906182144047No Hit
AGTTGGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1950.11201617628473937No Hit
GCCCAAGGGAATGAAGAATTCTGTTTAGCTGTGGGTGTGCCGGGTGGGGTGT1950.11201617628473937No Hit
GGCCGCGGGGGGGAAAGAGTTATTCCATAACAGAATTATTGCTTCCCATGGC1940.11144173435507405No Hit
GCACACGGGAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCACA1940.11144173435507405No Hit
AATAATGGGGCTGCCCCATATTTTAGAAAATGTAAAAATGGTGGTTTGGCCA1940.11144173435507405No Hit
TGCAATGGGCACTGAATTCACTGAGATTATTCCTATAATTTTAAAGTATCAT1930.11086729242540871No Hit
ATGCTTGGGCTGAAAGAAAGAGATGTAGAAGAATTACAGCTAAAACTTACAA1930.11086729242540871No Hit
CACAATGGGGTATGATTTTTAAAGAAAAATCAGGAATCAGCTTATAAGAAAA1920.11029285049574339No Hit
GTATGAGGGGGAGGAAAACTAAGCATTGGCCTCATGTTCAGTCTTCAGGATA1910.10971840856607805No Hit
CATACAGGGAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTGTCTCGCT1910.10971840856607805No Hit
GTCCAAGGGAGGTCAAGAACGTGGCCGATTACTACCCGGAGTACAAGCTGCT1900.10914396663641274No Hit
TGCACCGGGTGGTGGCTCATGCCTGTAATCTTAATACTTTGGGAGGCCAAAG1900.10914396663641274No Hit
TGCAGTGGGTGAACCTCTGTATGTACATCAATGGCCACTCATTTATCTAATG1900.10914396663641274No Hit
TGAAATGGGAAAAATTATTTGACTACTCCAAAAGAAGTTAAGAAAAGGCAGA1890.1085695247067474No Hit
AAGAATGGGAGGCTTTGGAAGAAAGAATACATGTGTGGTGACCTCTTTGAAA1890.1085695247067474No Hit
TATACGGGGGTGAGAATATAGCCTCCCACCCCATTAACTTCGCTCCCATGGG1880.10799508277708207No Hit
CATCACGGGCTTTTTCTCCTCTATGAATAATTTTATATTTCATGCTACTTCT1880.10799508277708207No Hit
GATGCTGGGACATAAATGCTGTTGTGGCAAAGATTCAGGCTAAAATCCCAGG1870.10742064084741673No Hit
AGAAGTGGGCGATCTCCTGACCTCGTGATCCGCCCGCCTCAGCCTCCCAAAG1870.10742064084741673No Hit
GCGTTAGGGAAATGTTTAATAGCATGTATATTTACATATTTAAGTATTCAAG1870.10742064084741673No Hit
CACTCGGGGCTGAGGGAACTGCACATTCATCCAATACCTCAGATGTGGATTT1870.10742064084741673No Hit
TGCGATTGGGCAGATATGATTTTCCTTTTTCGCATATATGGTTTAATAAATG1860.10684619891775142No Hit
AGCATTGGGCAGAATTTGTTAGCATACCCATTCATTATTAGTTTTACCTAAA1860.10684619891775142No Hit
TCACTGTGGGCAATGAATAGTTTTAGAGACACAAGCTTAGAATCCCAAAAGG1840.10569731505842075No Hit
ACGTATGGGTACGCAATAAAATATTGATGTTTGAAGGCAGTGGTCACCAATT1840.10569731505842075No Hit
GGAACCCTAGAAGATTGTGGGGATAGGTAATGGATGGATTCAACATGCCTTT1840.10569731505842075No Hit
TAACGCGGGCAGCCAGTGTCCATCCAAAAGGCCACAAATTGATTCTCCAGCT1840.10569731505842075No Hit
GCTCTTGGGCATTTAGACCCTGCAAGTTGATTCTCATTAATTGTCAGATTCC1830.10512287312875541No Hit
ACAGTTGGGAAATGACTAAATGCCAAGTCACTTAAATAGAATAGCAATAGAT1830.10512287312875541No Hit
GCATAGGGGAGAAAATTGTATTTTAAATCTTCTAACAGAAAAAGAAGTAAAT1830.10512287312875541No Hit
TCACACGGGGAATTCCCATTAATGAGCAGTCTTCAGTATTAAAACCACTGTC1820.10454843119909007No Hit
ACTGTGGGGCTTTAAAGTGATCATCAAAACTTTAAAACAGTGCTATTTACAA1820.10454843119909007No Hit
ATAGTAGGGCTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTG1820.10454843119909007No Hit
TTCGGCGGTACCCAGCTGAGAGGAGGACCAATAGAAAGAAAATTCACATTTG1810.10397398926942475No Hit
GCTGGGGGGCTTCAGAATACAGTTGTTTGCCCTGTGCATGAATATACCCATA1810.10397398926942475No Hit
TTCAATGGGTAACTAACCACTGGCTCTTTCTCGAAAATTTGGTAGCTGCCCC1800.10339954733975941No Hit
ACCCGGGTTGGAAATTTTTTAAAGATGAGATTTGCCTTTATAATGTAAATTA1800.10339954733975941No Hit
AAACCAGGGGAAAGTATCAATTGCTTTAAAACTGTTGTAAGTACAGTTGGCA1800.10339954733975941No Hit
AACAATGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1790.1028251054100941No Hit
GGACTTGGGGGGCATCCGGGACGGCGGGCGGGCTGGCCACCACGGGACAGGA1790.1028251054100941No Hit
CGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTC1780.10225066348042876No Hit
TTCGCTGGGATGCCTGGATGCCTGGGTAGCCAGTTTGGGGAGTGGGCTGCAA1780.10225066348042876No Hit
AATTTTGGGAACCTGATTCTAGTCGTGAGGAAATATTACCCAAATTTTGGGA1780.10225066348042876No Hit
GTCAAAGGGATTCCAACAAATTCACTGTCCAAAAGCAATATATAGAACTTGG1780.10225066348042876No Hit
ACACTAGTTAACGGTTTCCCACAGGTGTATTTTAATTCATTCTTAGGCATTT1780.10225066348042876No Hit
TACATAGGGAATATTTTATTACTTGGTTAAACACAAGAATTATCTATGTAAA1780.10225066348042876No Hit
ATTATAGGGGCTAAAATAGATGACATCAGATAATTTAATAACATCAATATAA1760.10110177962109809No Hit
AATCAGGGGATGCCATCAAAACTTATTAAAAAATAGGATTTACTTTTTTCTA1750.10052733769143278No Hit
ATAGATGGGCAGGCTTTTGATGTTGTGTCTTTCCACTCAAATGAATTTGCTA1750.10052733769143278No Hit
GTCAGCGGGTGCTGGTGGGCTGTCTCAAAACAAAAACAAAAACAAAAACAAA1750.10052733769143278No Hit
TCAGATGGGCTACAGTGGAGCCAGTCAACATGTGTACAACTTAACACCTAAC1750.10052733769143278No Hit
GAACTTGGGATCTGTTCCGCTTCTCAGCGGTCCATCTCAGCGTCTCCCTTCA1750.10052733769143278No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)