FastQCFastQC Report
Sat 31 Dec 2022
SRR5846360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173500
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC36992.1319884726224783No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT32631.8806916426512967No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT5460.3146974063400576No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4500.25936599423631124No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC2840.16368876080691644No Hit
CTTGATGGGAAGGAAAGCTTTTATCTGAATCCCAATTGAGAATCTGAATTCT2580.14870317002881844No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2570.14812680115273777No Hit
TTCGCGGGGTTCCTGAAATACCTTAAACTCTTCCTTGCCTCAGGAACTTTGC2550.14697406340057637No Hit
TATAAGGGATCCTTCAAGAATTAAGGCAAAATCAAGATCAAAATCTATCATT2480.14293948126801154No Hit
ATGGTTGGGTCCACTTCCTCACCAACATTTTATGTTTTTAGTCTCTTTAATA2480.14293948126801154No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC2460.14178674351585013No Hit
GCTAATGGGAGATGTATTTCCTGAAGCATATTTTTCATAGAATGTAGCGTGT2420.13948126801152738No Hit
TGACTTGGGATATCTATGTGTTACATGTCGATAGATAATATATATCTATGTG2380.1371757925072046No Hit
TTGAGCGGGGTATACTAACTCAACAGAATAAACTCATAACCACTAGCCTATC2340.13487031700288185No Hit
CTAGTAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2330.13429394812680115No Hit
ATCCCGGGAGTCACTGGTGACCTTGATAAAAGCAACTTCAGTGAAATGGTGA2330.13429394812680115No Hit
TAACGCGGGCAGCCAGTGTCCATCCAAAAGGCCACAAATTGATTCTCCAGCT2310.13314121037463977No Hit
TTTGCTGGGGGGGTGAGTTTATTGACTACCCAAACTGTATTTTACCAATAAA2300.13256484149855907No Hit
AATGGCGGGAGAATACGAGATTCAGAATATGAAATTCAACGACAAGCTAAAA2290.13198847262247837No Hit
GCACAGGGGGGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGT2280.1314121037463977No Hit
TTGTGAGGGTAGAAATGAGCAGATTGGTTAAACTACTCTGTTTTCTTAAACT2240.12910662824207494No Hit
TAATATGGGGGATACAGTTAAAACTGTGGATACAGTTAAAACTGATTAGAGG2220.12795389048991354No Hit
TGATAAGGGGCATTTCTCAAAGGATGGCTACCATTTTGCTGGAGCCAAAGAA2180.1256484149855908No Hit
ACCTTAGGGTGGATTTTAATGCTTTTATGCATTTTATCTCTGCTTTGATAAA2180.1256484149855908No Hit
TACAAAGGGTATGTGAAATGTATATTTTTATTTTTCTGAAATTGCCCTTAAA2170.1250720461095101No Hit
CGATTTGGGCCAAGAAAATGTTTGCCATTTTTGCATTGTTTCGTTTTTAACT2170.1250720461095101No Hit
CAGATGGGGGGTTTAGAAGGCATATTGAGTTATTTTGCAGAATCATAATGGA2150.12391930835734871No Hit
CCAACTGGGGCCCAAGCGGAGCTGCCCCTTCGCGGACGTGGCCCCGCTACAG2150.12391930835734871No Hit
ATGCTTGGGCTGAAAGAAAGAGATGTAGAAGAATTACAGCTAAAACTTACAA2150.12391930835734871No Hit
ACAAAGGGATCTGGACATTTGGTGATTTCTGGTTTCTGTTTTTTTGAGTTTT2130.12276657060518732No Hit
TGAAATGGGAAAAATTATTTGACTACTCCAAAAGAAGTTAAGAAAAGGCAGA2130.12276657060518732No Hit
TTGCTTGGGAGTAATCCTCTTTATAAATTAAACCAGGTTTGTTTAATATATA2100.12103746397694524No Hit
AGGTTCGGGGGAAAAGCCTGCATTTCATCAGCAGAAGAATGGATAAATAAAT2090.12046109510086456No Hit
TAACACGGGCAACACTCATTTTGTTGACATGTATCTGACCATGCTGATGTCT2090.12046109510086456No Hit
TAGTCAGGGAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACA2080.11988472622478386No Hit
GGAACCCTAGAAGATTGTGGGGATAGGTAATGGATGGATTCAACATGCCTTT2070.11930835734870317No Hit
GAACAGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2060.11873198847262248No Hit
AATGGTGGGCATGCAGTTGCTGCGAGACAACCTCACCCTCTGGACGAGCGAC2060.11873198847262248No Hit
ATGCTTGGGGGGTGAAGGGCGGCGCGCTTAGGCAGGCGGTGGCGCGGCTGGA2050.11815561959654178No Hit
GTCCAAGGGAGGTCAAGAACGTGGCCGATTACTACCCGGAGTACAAGCTGCT2040.11757925072046109No Hit
CGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTC2040.11757925072046109No Hit
TACCAGGGGGGGTATATGGTTAGTTTATTTGTTTCATTTAAAAAATCTCTTT2030.1170028818443804No Hit
GCTAAAGGGAGAATAATGAATTCATTACATGGAATGATATCCAAGCTTGCGT2030.1170028818443804No Hit
GGACTTGGGGGGCATCCGGGACGGCGGGCGGGCTGGCCACCACGGGACAGGA2010.11585014409221901No Hit
TGGCTTGGGAGACGCCATGGCTGCATTGAATGATATACGGTATCAGAGACTG2000.11527377521613834No Hit
AAACCAGGGGAAAGTATCAATTGCTTTAAAACTGTTGTAAGTACAGTTGGCA2000.11527377521613834No Hit
AAGAATGGGAGGCTTTGGAAGAAAGAATACATGTGTGGTGACCTCTTTGAAA2000.11527377521613834No Hit
TGCAATGGGCACTGAATTCACTGAGATTATTCCTATAATTTTAAAGTATCAT1990.11469740634005762No Hit
CTGCCGGGGGTCTTGAACTCCTGGCCTCCAGTGATCTGCCCACGTCGGTCTC1990.11469740634005762No Hit
GGATTCGGGAAGGGTGGAAGTTCTAATGTCTGTTAGCTACCCATAAGAATGC1980.11412103746397695No Hit
ACAGTTGGGAAATGACTAAATGCCAAGTCACTTAAATAGAATAGCAATAGAT1970.11354466858789626No Hit
TGGCCGGGGATGGAAACAAGAAATACAGAAAATAAGTCAATCTGAAAGATTT1960.11296829971181556No Hit
CAAATCGGGCATTCAGAGATATTTTAAGGACCTTGTTTTAACTTTGTTTCAA1950.11239193083573487No Hit
GCACACGGGAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCACA1950.11239193083573487No Hit
ACTGTGGGGCTTTAAAGTGATCATCAAAACTTTAAAACAGTGCTATTTACAA1940.11181556195965418No Hit
ACCCGGGTTGGAAATTTTTTAAAGATGAGATTTGCCTTTATAATGTAAATTA1940.11181556195965418No Hit
AGCTAAGGGACTCTGGCCAACTGTTACCAGTTGGTATTTCTACAAGTTCAGA1940.11181556195965418No Hit
ACCACGGGTGACTTCATGAAGTTGATTTTTAAAGGCAGCATCAAAAACTGAA1930.11123919308357348No Hit
GCGACAGGGTCTTTTGCTGAAGTCAGGATAGATTCAAGACATAATCTCTTGT1930.11123919308357348No Hit
TATACGGGGGTGAGAATATAGCCTCCCACCCCATTAACTTCGCTCCCATGGG1930.11123919308357348No Hit
CGTGCTGGGGAATCTAAGAATATAAAATTTCTAGGAGAAAATATGGAAGAAA1930.11123919308357348No Hit
GCGTTAGGGAAATGTTTAATAGCATGTATATTTACATATTTAAGTATTCAAG1930.11123919308357348No Hit
AATAATGGGGCTGCCCCATATTTTAGAAAATGTAAAAATGGTGGTTTGGCCA1930.11123919308357348No Hit
TCACACGGGGAATTCCCATTAATGAGCAGTCTTCAGTATTAAAACCACTGTC1920.1106628242074928No Hit
TGTGATGGGATAACATCAAAGTAGAAAAAAAGGTGTCTGTGCTATTTCTGTA1920.1106628242074928No Hit
ATTAGAGGGTTCTCTGGAATTGAAATACATCCTAAAATTGTTGGTTTGTCAT1920.1106628242074928No Hit
AAGATAGGGATATGGAATAAAATAAGTATTGAAGAATTTTTCTTTGTAACAA1910.1100864553314121No Hit
CTTATTGGGAAATGACAACTTTGGTGCTTTAAAATGAGGTACCACTTTTTAA1910.1100864553314121No Hit
TTTGTGGGGGTCATTTCAAGAATGTTATGTAAATGGAATTATACAATATGTA1900.1095100864553314No Hit
GCTGTGTCATTGCATGAGCAACTTTGTGTCTGTGCTTTTTCTACAATTTGGA1890.10893371757925072No Hit
GCCCAAGGGAATGAAGAATTCTGTTTAGCTGTGGGTGTGCCGGGTGGGGTGT1890.10893371757925072No Hit
ATAGTGGGGAGAACTTTAGACCCTGTAGCCATAGAGGGAATGGCGTTCCTTT1890.10893371757925072No Hit
TTGTCAGGGCAAACATCATCTTTATGTTTAGTCAACTGTTTTCATGGGAAAA1880.10835734870317003No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1870.10778097982708933No Hit
ACTGTCGGGGATTTTCCGACCCGGGCAAGATGGCAGCGGCGCTGCGCGTGCG1870.10778097982708933No Hit
TCACCTAGGGGTCTGAAGAATGATTTGATCTCAGAAAATGTTGGCAAAACCA1870.10778097982708933No Hit
GATGCTGGGACATAAATGCTGTTGTGGCAAAGATTCAGGCTAAAATCCCAGG1870.10778097982708933No Hit
AGCAACGGGGGTGTTTTTAAGGGGGTCTAAGCCTGCCTTTTCAATGATTTAA1860.10720461095100864No Hit
TTCGCTGGGATGCCTGGATGCCTGGGTAGCCAGTTTGGGGAGTGGGCTGCAA1860.10720461095100864No Hit
GCTCATGGGGTCCTGTGAGCATTCAGTCAGTTGAAATTAAAGATTCCTCATT1860.10720461095100864No Hit
CACCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1860.10720461095100864No Hit
TAATGCGGGATGTTGGGATCACGACGCAGAAGCCAGGTTATCAGCTGGATGT1860.10720461095100864No Hit
ACACTAGTTAACGGTTTCCCACAGGTGTATTTTAATTCATTCTTAGGCATTT1850.10662824207492796No Hit
TAAACCGGGAATAGACCACGATGTTACATCCGCAAAGGATGGTGATTACTGG1840.10605187319884725No Hit
GTCAGCGGGTGCTGGTGGGCTGTCTCAAAACAAAAACAAAAACAAAAACAAA1840.10605187319884725No Hit
GCAATTTCCCAGAAGCATTTGTCTCTAACCCATGTGGGTAGCCCGACCATGA1840.10605187319884725No Hit
CACAATGGGGTATGATTTTTAAAGAAAAATCAGGAATCAGCTTATAAGAAAA1830.10547550432276658No Hit
CTTCACCGGGAAGACAAGACAAACTGGGAGAAGATATTTGCCATGCATACAA1830.10547550432276658No Hit
GTTTTTGGGCATCAAGCTACCAATGACTTTCTTCACAGAATTGGAAAAAACT1820.10489913544668589No Hit
TGCGATTGGGCAGATATGATTTTCCTTTTTCGCATATATGGTTTAATAAATG1820.10489913544668589No Hit
CTTGCTGGGATGTCTTCTTGGAGAATGGACCCCTTTCATCATTATCCCTGAT1820.10489913544668589No Hit
GAACTTGGGATCTGTTCCGCTTCTCAGCGGTCCATCTCAGCGTCTCCCTTCA1820.10489913544668589No Hit
GCATAGGGGAGAAAATTGTATTTTAAATCTTCTAACAGAAAAAGAAGTAAAT1820.10489913544668589No Hit
TTGCCGGGCCACCTCCTGGATTCAAGCTATTCTCCTGCCTCAGCCTCTCAAG1810.10432276657060519No Hit
AAAACCGGGGGGCTACAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGA1810.10432276657060519No Hit
AACAATGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1810.10432276657060519No Hit
GGATTCGGGAAGTCCCAGTGGGAAGGGCTGGAAATCACACGTGGTCAACTTC1810.10432276657060519No Hit
GCGCAAGGGATATGGAAAACATTTTCTCCTCCTTTCTTACTTCTCATAAAGT1810.10432276657060519No Hit
GCATGAGGGGGACTGACATGTGTAGGAAAAGAACCGAACCACACAGTGTGGA1800.1037463976945245No Hit
ATAGATGGGCAGGCTTTTGATGTTGTGTCTTTCCACTCAAATGAATTTGCTA1800.1037463976945245No Hit
TTATACGGGTAGAGTCTGAAGATTATGCTTTGAGAAATCTTATTTTGGAAGA1790.10317002881844381No Hit
CCTAGCGGGCCGGACAGCAGCTTCAAGTTGACCTCTTAGAAGCCCGACCCTA1790.10317002881844381No Hit
GACTACGGGTACACTGCGAATTACTACCATCTATTCTCACTGCCAATGGAAA1780.10259365994236311No Hit
GACTATGGGGACTTCCTGAGCAGAAATGGGCGAACTTCAGTCTTTTTACTGT1780.10259365994236311No Hit
AAGTGGGGAGGCTGTGTGTGGGGAAAGGGACATATGAGAAGTCTGTACTCTC1780.10259365994236311No Hit
CTGTTAGGGGTGCAGGGCGGGTGGAGTCGCGGAGTAGTCCTCATGGCCGCCC1780.10259365994236311No Hit
TTTGCAGGGAACTCACTTTAAACTGGTCTCTGATTCCTCATCTGTCAAATGA1780.10259365994236311No Hit
TCTTCAGGGTCAATTCCTTTTAAAATGATGGTTGTGAAGACTGATGAAAACA1780.10259365994236311No Hit
CGTACAGGGGATAGCCATGGCAGCTCAGATGGCAGCCCTGCAAGCTAAAGCT1780.10259365994236311No Hit
CTCATTGGGCGATCTCTGGCGGGGAGTCCTCGAAGGCTCGGAAGCCCGTCAC1760.10144092219020173No Hit
AGTAATGGGAAGGCTGAAAGGAACATTCATACTGGAAGTAGTAGCAGTAGCA1760.10144092219020173No Hit
AGAAGCGGGAGTATCGTGGTGGTGCAGTGGGCCGACTTGGTCATCTGTAAGA1760.10144092219020173No Hit
GTTCGCGGGCGATGATGCTAAGGCACTGATAGCGGACAGTGGACTTAAAATA1760.10144092219020173No Hit
TAAAAAGGGTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAAT1760.10144092219020173No Hit
AATGCTGGACAGAAAGCAAATCAGACACAATTAGAGAAGCCTTTTCCATAAA1750.10086455331412103No Hit
ATTTGTGGGTCCCTTATTTAATAAGCAAAATATGTTTTTGGAATAAGTGGTG1750.10086455331412103No Hit
GGACACGGGGAATATGTCATAACCAATAATCCATGGGTGAGACTAGCTACAG1750.10086455331412103No Hit
ATAGTAGGGCTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTG1740.10028818443804034No Hit
AGTTGGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1740.10028818443804034No Hit
AAAAATGGGAAAATGTGTATGATATATTTGAAAAGGCTTCCATTAATATAAT1740.10028818443804034No Hit
AGCCTTGGGATGTGGAGGTAAAATATACAGATAACTGATCAGTTTGAGTGAC1740.10028818443804034No Hit
CATACAGGGAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTGTCTCGCT1740.10028818443804034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)