FastQCFastQC Report
Sat 31 Dec 2022
SRR5846356.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846356.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110951
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC20651.861181963208984No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT17331.561950771061099No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC6180.5570026408054006No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC5970.5380753666032754No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA5070.45695847716559557No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA3050.2748961253165812No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT1840.16583897396147848No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCT1570.14150390713017458No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC1540.13880001081558527No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1450.13068832187181728No Hit
ACGCTTGGGGTGCATGTTTTAATCTGAAAGAAACTATCAAGAAGCTCCCAGG1440.1297870231002875No Hit
GTAAATGGGAGTATTTATTGATGTATAAAAATAGGATAATGGGGAACTGGAC1440.1297870231002875No Hit
GTACCCGGGCAGACGAAACTGACAAATTTATCATCATAATGGAACATTTCAC1380.12437923047110887No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGT1360.12257663292804932No Hit
TAAGTGCGGGAGAATATGAATATTTCAGAGGAATAATTCACAGTGGAGATGG1310.11807013907040044No Hit
GTACTAGGGATTCTGTTCATGTATATCCAAGAGCAAAATTGTTTAATGGTTT1290.1162675415273409No Hit
TTAGTTGGGATATGCTCATGTCTCCACACAACCTGACATGCTGTATCTCTCC1290.1162675415273409No Hit
TGCTAGTGGGCCACTTTTTTATGGGATTGTTTGTGGGGTTTTTTGTACATTT1250.1126623464412218No Hit
GCCAATGGGCGATCTGTTGGCGGGAAGAATTAGAGACAGTAACTCCAAAAAT1240.11176104766969203No Hit
ATGCGCGGGAAAGATGGTGAGGCAAAGATCAAAAGCGACTATGCACAACTTC1220.10995845012663247No Hit
TAACTCGGGAACTCAGAAAGAAAATTAAAAATTGTTGTTTTAAACCACAAAG1210.1090571513551027No Hit
AAGCGGGAACTGCAAAATATTTGAGAAACAATTCAGTCTCTCAAACATTTTG1200.10815585258357294No Hit
TCATTGGGCATGAACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCTTTGA1200.10815585258357294No Hit
TTATCCGGGAGAATAAATAGAGTAATTTATGTATAGATACAAAAATATAAAA1200.10815585258357294No Hit
TTTCGCGGGGATAGAGGAAAATTTATAGCACTAAATGCCCACAAGAGAAAGA1190.10725455381204316No Hit
GTACCAGGGATTCAGCAGGCTGCTAGTCATGTCTGCTACTTTTCTAGCTTGC1190.10725455381204316No Hit
TTGCACGGGCTACCCTTTGGCTTGGCTTGACCTCTCCTTGTGAGCTCACCTG1180.10635325504051339No Hit
TCGTTCGGGGGCTCTTCCGATCTTGAAAAGGGCTGCTTGCTTTCTGGGGACA1170.1054519562689836No Hit
GTGTGCGGGCTGTTGGATTTACTCTGCTTAGAAGCTTTAATTGCAAAAAAGA1170.1054519562689836No Hit
TTTCAAGGGCGCTTGGCCATGCCTGGATCTGTTTTGTTTTGGTTTTTGGTTT1170.1054519562689836No Hit
TTACGAGGGCAGAAAGACTTCTTCAAGTACAATCTTTGTTGGATAGAAAAAT1170.1054519562689836No Hit
CGCACTGGGCACTGGAAAATGAAAAGTTAAAGGATGAATTACTGAAATTGTA1170.1054519562689836No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT1160.10455065749745383No Hit
TTGACAGGGAGGACTGAAGGCACAGGTGAAGGTGAAGCTGCTCATGTAGTTT1150.10364935872592404No Hit
CTAGACGGGGATTTCCCACCGGGGACTGGACACAAACAGGAATGTCTGGAGT1150.10364935872592404No Hit
TCTCGCAGGGCAAGCGGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTT1150.10364935872592404No Hit
GTAGCGGGAGCCTGAGTTTCTGCTAATGAGGCTGTAATTTTGGCACATTTAC1150.10364935872592404No Hit
CTGTCAGGGACAACAAGAAATGGGCTAATGCTATGAATAATAATAACAAACA1140.10274805995439427No Hit
TCTGCTCGGGGTCATAATTTATTTATTTATTCCTCCATTGAGAGCACATTTA1120.10094546241133473No Hit
TGTAAAGGGCTAGTAAATACTGTTACAGGATTCATGAACTTGAATAATTCTA1110.10004416363980496No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)