FastQCFastQC Report
Sat 31 Dec 2022
SRR5846354.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846354.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109819
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC19991.8202678953550842No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT16881.5370746410001912No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC6500.5918830074941495No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC5550.5053770294757738No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA5220.47532758447991696No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA3120.28410384359719176No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC1840.16754842058295924No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT1680.15297899270618015No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCT1520.13840956482940112No Hit
TATTTAGGGCGATGAAGCCAGAATTCCCTTGATACCAAAATCACACAAGGAC1440.13112485089101159No Hit
GCCAATGGGCGATCTGTTGGCGGGAAGAATTAGAGACAGTAACTCCAAAAAT1420.1293036724064142No Hit
GGCTGCGGGACCGGCACTGCTGCTGCACCTTTAATAAAAGACTTTAATAAGA1400.1274824939218168No Hit
GTACCAGGGATTCAGCAGGCTGCTAGTCATGTCTGCTACTTTTCTAGCTTGC1380.12566131543721942No Hit
GTAAATGGGAGTATTTATTGATGTATAAAAATAGGATAATGGGGAACTGGAC1330.12110836922572597No Hit
TGCTAGTGGGCCACTTTTTTATGGGATTGTTTGTGGGGTTTTTTGTACATTT1330.12110836922572597No Hit
TTAATAGGGGGAGGACGGGACAAAAAAATGCATTTGGGAGAAGTCATTTGTT1310.11928719074112859No Hit
TTTGCTGGGATCTTAGAACTCATTTCAGGTTTGTAATATCCAATTAAGATTT1310.11928719074112859No Hit
TCAGCTGGGGACGTCTGTGCCCTAACAGAAGCACCGTTCAATAGAACAGCAA1300.11837660149882989No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1290.1174660122565312No Hit
TCGTTCGGGGGCTCTTCCGATCTTGAAAAGGGCTGCTTGCTTTCTGGGGACA1280.11655542301423252No Hit
TACTTTGGGGATCTCTCATAGGGATTGCTGACTAACTAACTACAAATGCATC1270.11564483377193382No Hit
GGTATAGGGATCCAATAAAATATAATATTATTATAGATGGGTGATCACAAAT1270.11564483377193382No Hit
TAAGTGCGGGAGAATATGAATATTTCAGAGGAATAATTCACAGTGGAGATGG1250.11382365528733644No Hit
ACGCTTGGGGTGCATGTTTTAATCTGAAAGAAACTATCAAGAAGCTCCCAGG1230.11200247680273905No Hit
CGCACTGGGCACTGGAAAATGAAAAGTTAAAGGATGAATTACTGAAATTGTA1230.11200247680273905No Hit
TTGACAGGGAGGACTGAAGGCACAGGTGAAGGTGAAGCTGCTCATGTAGTTT1220.11109188756044036No Hit
TTCTGAGGGCAGAGACTGGTGGTGGAGCTGCTTATCAAAAACGAAGACATTT1220.11109188756044036No Hit
TCAGGTGGGCTACTTGATTAAAAAATATAAAGAACTGAGTGTTTATTTCCCT1220.11109188756044036No Hit
AAAACAGGGATTGTTCATCCAAGAAGGGTTTTTGTGACTGAATCAGCAGTGC1220.11109188756044036No Hit
CTGCGTAAGGGACCTATGTTAAATACATAGATTCTTTTTTTCTTCCTTTGAT1210.11018129831814166No Hit
TGATAAGGGATTAGCAAAATTGGTATAATTATGGCAACATCTACTCCACTAA1200.10927070907584296No Hit
CTGTCAGGGACAACAAGAAATGGGCTAATGCTATGAATAATAATAACAAACA1190.1083601198335443No Hit
ATAGGTGGAAGTAAAAGACAAAATATTTTGTGGTGGGAGCAGTAAGTTAAAC1180.1074495305912456No Hit
GTGCTGGGGATGCATGTGCTTGAAAATGACATTTTATTTCCCGGATGCTGTA1180.1074495305912456No Hit
TTGCACGGGCTACCCTTTGGCTTGGCTTGACCTCTCCTTGTGAGCTCACCTG1180.1074495305912456No Hit
TCACTAGGGTGAAAGTCACATGACTGAGTAAATTCACCTAGGGAGAATATAT1180.1074495305912456No Hit
TGACTATGGGATACTCGGATATTTGGGTCTTTTTGAGAGAGCTGGGGAAAGT1170.1065389413489469No Hit
GTACCCGGGCAGACGAAACTGACAAATTTATCATCATAATGGAACATTTCAC1160.10562835210664821No Hit
TTTCGCGGGGATAGAGGAAAATTTATAGCACTAAATGCCCACAAGAGAAAGA1160.10562835210664821No Hit
CTTGATGCGGGTCCATGTTAATGTTTCAGTTAAGTAGACGAAGGAATTCAGA1160.10562835210664821No Hit
CTCTCTTAGGGAGAAATGCAAAATGCTCTGGAAAGTCTCAGCAATAGAATTG1160.10562835210664821No Hit
ATGCGCGGGAAAGATGGTGAGGCAAAGATCAAAAGCGACTATGCACAACTTC1150.10471776286434951No Hit
ACAGTCGGGGTTTCTGAATGTGAACCCAAAGACCCTACCCCTGGAGAGGAAG1150.10471776286434951No Hit
TTACGAGGGCAGAAAGACTTCTTCAAGTACAATCTTTGTTGGATAGAAAAAT1150.10471776286434951No Hit
ATTGCAGGGAATATTTTTCTATGTACTCAAGTACCTTTTTACTTCAATAAAT1150.10471776286434951No Hit
GTACTAGGGATTCTGTTCATGTATATCCAAGAGCAAAATTGTTTAATGGTTT1140.10380717362205084No Hit
TTGTTTGGGTAATGAAACTGGTACCATCTGTGCTTTCACAAAAAACTTCCAA1140.10380717362205084No Hit
TCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCGCCTGGCCTA1140.10380717362205084No Hit
AGCATTGGGAGAATAGAATAAGTACATGCTGCTGTAATAAAATTGCCTTTAA1130.10289658437975215No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGT1130.10289658437975215No Hit
GTAGCGGGAGCCTGAGTTTCTGCTAATGAGGCTGTAATTTTGGCACATTTAC1130.10289658437975215No Hit
TAGGTTGGGCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCGCGCC1120.10198599513745343No Hit
TTAGATGGGGATCTAATAGTGGGAATGTTAACTTCTTTCTTCCAGCCTTAAT1120.10198599513745343No Hit
TGTAAAGGGCTAGTAAATACTGTTACAGGATTCATGAACTTGAATAATTCTA1100.10016481665285606No Hit
AAGCGGGAACTGCAAAATATTTGAGAAACAATTCAGTCTCTCAAACATTTTG1100.10016481665285606No Hit
GCGTAGGGGGCGGGACCACAGTGGTGTCCGAGAAGTCAGGCACGTAGCTCAG1100.10016481665285606No Hit
CACACACAAACTGAGAAAAGATTTGTATCAAACAGAATCAGAGGCATATGAA1100.10016481665285606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)