FastQCFastQC Report
Sat 31 Dec 2022
SRR5846341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42343
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC21515.079942375363107No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT15193.5873698131922636No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC6931.6366341544056868No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA4401.0391327964480552No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC4281.010792811090381No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA3760.8879862078737927No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT1890.4463547693833692No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC1740.41092978768627636No Hit
GTACCAGGGATGTGGTCATAGTTGACTGCTGAGAAAAGGACAATGAAATGAT1260.2975698462555794No Hit
CTAAGTGGGATGCCTATATTCCCAATTATATTTTTCTAACTACTTCAGGACA1200.28339985357674236No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGT1180.2786765226837966No Hit
TCACTAGGGATCTCTGGACTGCAATGGTCTTCTTGTGCTAGAACTCAGGCTC1170.2763148572373238No Hit
TGCGAACGGGAATTATTTCTTGCTAAATTCCAAATATTTGAAATTTGAAGAA1170.2763148572373238No Hit
GCGCCCGGGTCTATTGGAGGCGCCCCATCTCAGACTTCCTAACACAGCCTGT1160.2739531917908509No Hit
GAGATAGGGCAAGAGGACAGTGATGGTCACTTAAGTGTGAATTTGGAGTATG1130.26686819545143237No Hit
TTGTAGGGGGCGCACGCGCAGTGACGCGCCGGCCATGCCGGCGGCTGTTGTC1110.2621448645584867No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCT1100.2597831991120138No Hit
CTCAGTGGGGGCAGGAAGTTAACCTATTTAACAATTAGAGCTAGCATTTCAT1100.2597831991120138No Hit
AACTGTGGGGATATTAATCATCTTTGAGTAATTCTTCTAATCAAACATGCTC1060.2503365373261224No Hit
TTGAGCGGGGAGCCCTGCTGCTGGGGTTGATTTTGAACTGGAGTCATTTTCT1060.2503365373261224No Hit
ATATATGGGATAATAATCATTTCAGAATTTTACTCTGCATCACAATGTATTT1060.2503365373261224No Hit
AACTCCGGGAACTGGGTAAAAAGAACACAGGATCTCTGTATTATTTCTTACA1060.2503365373261224No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1050.24797487187964953No Hit
AACGATGGGGCAAATATTAAGATATGAAAGGATGATAAAATAAGTATTTCTG1040.24561320643317666No Hit
GATGTAGGGATGACCAAGCAAAAAGACTTTAAAAAATGGTAATGAAAATGGA1030.24325154098670385No Hit
CGTCACGGGCCAGCCTTACTGGGTCCTTACCCTGGGCCAAACAGGGAGGGCT1030.24325154098670385No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCC1020.24088987554023097No Hit
TAACAGGGGTAGGTAAATAATGGATTCCTATAATGGGAGCATCACCACTTAT1020.24088987554023097No Hit
CAAACAGAGGGACCGGCTGGAGCCACGGCAGAGAAAACATAAATTGTGAAGA1010.2385282100937581No Hit
TAGCTTGGGCATTTAAATATTAATCAACTTTCCTTAAAATAAATAGCCTCTT990.23380487920081242No Hit
AATGCTGGGATATAATGTATTTCTAGGTTCTGTGTTATGTTTTTTGTTGAAT990.23380487920081242No Hit
CTACGGGGGGTCGCGGGGCAGACTAATCCCCTGCTCCTGGCCAGGGGAGGCT980.23144321375433954No Hit
AGTTGCGGGAGCAAAAGCACAGAGACTTAAGACTACTGAGTTGTGAAAATTA980.23144321375433954No Hit
CGTTGCGGGGCTCTGCGTGTGCAGCTCTCTGCGTCCCTCCTGGGCCCTCCAG970.22908154830786673No Hit
TTATGAGGGACCTGGGTGTCTGGGCTCAAGCTTTCCCGCCCAGCCTCACTTC970.22908154830786673No Hit
AAACGCGGGAGACAGGACCAACTTCAGATTCCCAAAGAAGCCAGCTACAGAG960.22671988286139386No Hit
GCAACAGGAAGGGAAAGGACAGGAAATGGAATTCTTACAGGGAAGTAAGTAA960.22671988286139386No Hit
TACTGGGGGAGCAAATCCAAAGCAGAACTTAATGTCTCATATTAGAAGAATA960.22671988286139386No Hit
GTTTACGGGAGTTATAGTTACGAGAAAGATATGGTACAGGGAGGAAAATACT950.22435821741492099No Hit
ATGATAGGGCAGTCACCGGCTTCGTCCTTAGTTTTCCCAACTTGGGACGTGA950.22435821741492099No Hit
TTTGATGGGCACCACAAAGAAGTACCTTGTCCCAAGTGTGTCCGGGCCTGAT930.2196348865219753No Hit
TGGCTGGGGAGTGTCTATTTCCTGAGGAACCCAACTTATAACACGTAGAAGA920.21727322107550243No Hit
TGCAGCGGGCAACATGATGCAGTTATCTTGCATACAACTGAAGACAACTAAC920.21727322107550243No Hit
CATGGCGGGACCGCGCGGCGGCCGCTGCGGCGCGATGGCGGCGGGGCTGGCG910.2149115556290296No Hit
AGCTGCGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTGT910.2149115556290296No Hit
ACTTTCGGGCTAAGAATGCTTTGCCATAACCACATTTAATGGGAAAAACGGC890.2101882247360839No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACCTCGTATGC890.2101882247360839No Hit
TCACGCGGGCAGTGATGAGCACTGCACCTAACAACAGCAGAATGTTCTTCTC890.2101882247360839No Hit
GCCAGCGGGAGTCTATTTCATGGGATGAGAATGGAACTTGCATAGTGATTAA870.20546489384313818No Hit
TGAACAGGGCGCGCTTTGTCCTTAGCGCCTGTCTGCTCTCCTCTAACTAGGA860.20310322839666534No Hit
CGGGCTGGGGTGGTTGAGATGGAAACCTTTCTCGCAAGCTTATTGAATATTT850.20074156295019246No Hit
CGAATTGGGGAGTAACATAGGAGACCAAGAGGAAGTCAAAAGCTTGCTCCAA830.19601823205724678No Hit
AAGTATGGGGTGCTCCTAGGGTTCTCCAGGTGGTTCTTCAGACCTTCACCTG830.19601823205724678No Hit
GTAATTGGGGAGTTCAGAGCCTTTAAAAACATGATGTCTTCAGCAGAAAACA820.1936565666107739No Hit
AACTAGGGGGCTGGTGAATAATTTTTTTGTCTTGCTTAGTGGCAAAGTGGAT820.1936565666107739No Hit
ACTTTTGGGGTCCATGCCTGCCTTTTGTTGTTTACATGCTCTTCTGCCCAGA810.19129490116430106No Hit
TAATTGGGGGCTTTTCATTTGTCCAAGATGAGTACATAGCAGAATTTGGAAA800.1889332357178282No Hit
GCTAACGGGTGCTGCTTCGGGGCTTAGGCCACCGCTCCCTGTCCCCAGAGGA790.18657157027135535No Hit
GCTAATTTTTTTGTATTTTTAGTAGAGACAGAGTTTCACTGTGTTAGCCAGG780.1842099048248825No Hit
TATGCAGGGATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAG780.1842099048248825No Hit
ATATCCGGGTCCATAAACCAAGTTAAAGTATGGCCCGACCATTTAAGAAAAC770.18184823937840966No Hit
AAGCATGGGGTGCCCAGTGCTTAGCTCATAAAAGATGCTAAATTAATGTTAT770.18184823937840966No Hit
AAGCAGGGGGCCTCCTCCAGCCCCTCTCATGGCACCAATGGAAGTGTCCTCC760.1794865739319368No Hit
TAAAATGGGGGTCCCTCCTTAAATGTCCGGCGGGGAGGGGCCTGTGACCTGA750.17712490848546394No Hit
TCGCCGGGGTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGG750.17712490848546394No Hit
TGCTTTGGGAGTGCCCCAGGGGCCTCCTGGGCCACGGGCTGGAGGGGCTGCC750.17712490848546394No Hit
GCGACTGGGTTAATAGAACGTGATTAGTCATCGCCGAGAAGAAAGCATATTA750.17712490848546394No Hit
CTGATTGGGGGGTTAAAAATATCTTTTCTATGTTGGTGTAAACTTTTTCGAA740.1747632430389911No Hit
AGTACCGGGAGGAGAAGCACCGGGGCCTCACCGCCCCCTTTCTGCAGCCCTT730.17240157759251823No Hit
ATACAGGGAGACAAGCCAGTGAGCTCTCCCCTTGGGATCACTGGGGTGATCA730.17240157759251823No Hit
GCAACAGGGAGGAGCGCAGGGACGGCAGGGCCAAGCCCGAGGAGGTGCACCG730.17240157759251823No Hit
CTACAAGGGCGATCTTTTGAAGGGCAATGATGAAAAGCCCGACTGTACAAAC720.17003991214604539No Hit
ATAACTGGGAGAGCCTTCTCCACCGCCGGGGAATATTCATGTGGGAAATGGA720.17003991214604539No Hit
GATTTAGGGGCGGCCAGTAGAGGGATTCTGGGTAACGGCCCGGACCCCCGGC720.17003991214604539No Hit
ATCATGTGGGTCTGGAAAAGGGATGTCAGTAGAATCAAGGCAGTTTAAGGTT710.16767824669957254No Hit
CCATCACGGGGGGCTCACTGCTATCTCCGCCTCCTGGGTTCCAGTGATTCTC700.16531658125309967No Hit
CTAAAAGGGAGGCATTAAAAGAACAAGTTTCCAATGCAGACTGGTGTTTGGG690.16295491580662683No Hit
TGTAAGGGGATGGCAGCCCGGCTGCTGCTTGGATGCGAGCTTGGCCTTGGGC690.16295491580662683No Hit
TATGTGGGGATGCAGCAAAGGTGCTGTGAGCCCAGTTCCAACGGCTGTGTTC690.16295491580662683No Hit
CTTTACACGGGGAGACTGGGGTGGAGACTTCGGAGACTGCAGTTGCAGTTGT680.16059325036015398No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTC680.16059325036015398No Hit
TACGCAGGGTGAGTTTCTTCTTCCCCTTGAGCTTCAGAGAGGAGAGTTGGCA680.16059325036015398No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT670.15823158491368114No Hit
TAGCTGGGGCCATTCTGTTATTCTTATGTATAACCACCTTTTAAAAATATTA670.15823158491368114No Hit
GGGAACGGGGCTCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCA660.15586991946720827No Hit
TTCCGTGGGGGACGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGC650.15350825402073542No Hit
ATGCGGGCTAGACCTTGATCAACTCACTTTAAACTGGTCTCTGATTCCTCAT650.15350825402073542No Hit
CGTAGTGGGCTGCTAGAAAACACATTGTACTGTGCTTTTTCTGGAATTCAGT650.15350825402073542No Hit
TCTGTGTGGGCAAATCTGTCAAGGTTATCAGAAGCAAGCAAAGTCTGAGAAA640.15114658857426258No Hit
TTATCTGGGATTTATTTTAAAAAAAAGAGAGAGTAACTACAGAAGAACTTTA640.15114658857426258No Hit
GCAATAGGGGAAATACCACTTGTGTCCCTCCTCAAGTTCTCTGACCACCTTC630.1487849231277897No Hit
TGTAAGGGGACGCTCTTCCGATCTTATCATGGGTTGTATGTTAAAGTGACTG620.14642325768131687No Hit
TTCACTGGGCTCATAGATAGTAATTACCAATGTAACTAAGTATTTGTGTTCT620.14642325768131687No Hit
TCAGCTGGGAAGCATGGCCCTGCTGCCCCTCCCTGCCTCCACCCACAGTGGA600.14169992678837118No Hit
GTTTCTGGGTACTGAACTTGGAATCACAGGACTGGGGAATATTCATTGGGGA580.13697659589542546No Hit
CACTGCGGGCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGTGCCCGCC570.13461493044895262No Hit
TGCGTTGGGGGACCACCAAGGGGTTGTTGAAAACTAATTGTATTAAATGTCT560.13225326500247975No Hit
AGCGCTGGGAACATTAATGTACTGTGAATCGTTCCTGATAAGTGAATAAAAC560.13225326500247975No Hit
GTACTAGGGATGACACATTCTGAAATGAGAAAAGAACAGAGCCATGAGGACC550.1298915995560069No Hit
GTACTAGGGCTAGGATGTAACAGTGAAGCTGAGTAAACTATAACTGTTAAAC550.1298915995560069No Hit
ATCACCGGGTAGGGAGCCCTCCCATTGCCTTCCTGGCCCGGGATAGAAGAGG540.12752993410953406No Hit
CTCACTTGGGGGGGGTGGGCTTGGCCGAGCTGGCCTCCGGGGCACCGACCGC540.12752993410953406No Hit
CTCTAAAGGGCCAAAGACTCGGGAGAACTGGAAAGGCTGTCTGGGGTCTTCT530.1251682686630612No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAAT530.1251682686630612No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTC530.1251682686630612No Hit
TGCTCCTGGGAGGTGCTATTTAAGAGTAAACCAAAGGATAAGCAGAGGGAGT520.12280660321658833No Hit
CTTGTTGGGGAGTATCAGGATTTAGTGACCTAATTGAAGATGTCAAGATGGC520.12280660321658833No Hit
TTGACCGGGGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCA510.12044493777011549No Hit
TTAACACGGGATGTCTGTTGTATTTTCAGTAATGTGATTTCAGATGGTCATC510.12044493777011549No Hit
ATTGCCGGGGGTGTTCCTCATATTATGGTACAGGGCCAAAGACTTGAGACGT500.11808327232364264No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCG500.11808327232364264No Hit
AAGCCTGGGCAACAAGAGCAAAACTCCATCTCAAAAAAAAAAAAAAAAAAAA500.11808327232364264No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATC500.11808327232364264No Hit
TTTTATGGGAACACATCTGCTTCCAAAACTTTTTAAAAAGCTTTTAGTGTGG490.11572160687716977No Hit
TAGCAAGGGCAGTGATCATTAAGACAGGTAATTTAGGTGAAAGCATTTTAGA490.11572160687716977No Hit
ATTCAAGGGTGAAAGAAGCTGTGCTGTACGTGGATTCCCAGGAGGCTGCCCT480.11335994143069693No Hit
TTCTACGGGACTGTTTTTTTGTGAGAATTGTCAGTGTAACTATTACCTACCA480.11335994143069693No Hit
CTTAGTTGGGAGATTTCAGTTATTGAATACTTATTCTGTGGCAGTCACTCTT470.11099827598422408No Hit
TAATGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC470.11099827598422408No Hit
CGTTTGGGGGGCATCTGGTGAGTGTAATTCCATGCCAGAAGGGCAAAAGGTG470.11099827598422408No Hit
AGTCCAGGGGAGGACGGGGCGGAGCTTCCGCCTGGGCTGGGGTTGGCGAGTT470.11099827598422408No Hit
AGTAGCGGGATGGGAGATTTCTAAATCTATTTGTCTCAGTGAAATAAGAGGG470.11099827598422408No Hit
GTTAATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGA460.10863661053775121No Hit
ACCCTGGGGAAATTTAGATTTTTAAAAAAATGAATTTCACAGTGATTCTTCA460.10863661053775121No Hit
AAGTCGGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGGTGAGTG460.10863661053775121No Hit
GGTAACGGGGACAGCTCTTTGGGCGTTGATTCTAGGGTCAGGGCCCTCACTC450.10627494509127837No Hit
TACAAGGGGGTTAGGGTGTTAGTGATAAGTATGCATAAATGTCAAAGATGGG450.10627494509127837No Hit
GAAATTGGGGGAGGAAGGAAAGGTCGCAGCCTTCAAGGAGGAGCACATCTAC450.10627494509127837No Hit
AGAATCGGGGTCCATGAGGAGAAAAAAAGCATGTGTCATGCATGAAGGAAAC450.10627494509127837No Hit
CATCACGGGTCTAAGGAAGGGGAGAGGTGAAGTAAAACCGATACCTGTCCTG440.10391327964480553No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATG440.10391327964480553No Hit
ACTTGAGGGACGTGTTTCTGAAGTGGTTTAATCGTATTTGTTTAGCTTCTTT440.10391327964480553No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACCTCGTATGCCGTCT440.10391327964480553No Hit
AAGAACCGGGGGGCTGCCCTTTCGAGAACACCCTGTGGCAGTTCAGAGACAC430.10155161419833267No Hit
CTTATTGGGGCTCTTTCTCGACTCCATCTTCGCGGTAGCTGGGACCGCCGTT430.10155161419833267No Hit
CTTTAGGCGGGAAGTGTTGGGAACTCTCTGCCAGCTTCCTGAGTTCTGCCTA430.10155161419833267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)