FastQCFastQC Report
Sat 31 Dec 2022
SRR5846340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41710
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC21045.044353871973148No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT15703.764085351234716No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC7951.9060177415487891No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC4601.1028530328458404No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA4120.9877727163749701No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA3800.9110525053943898No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT1800.4315511867657636No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC1430.3428434428194677No Hit
GAGATAGGGCAAGAGGACAGTGATGGTCACTTAAGTGTGAATTTGGAGTATG1270.30448333732917765No Hit
TTATGAGGGACCTGGGTGTCTGGGCTCAAGCTTTCCCGCCCAGCCTCACTTC1240.2972908175497483No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGT1220.292495804363462No Hit
GCGCCCGGGTCTATTGGAGGCGCCCCATCTCAGACTTCCTAACACAGCCTGT1210.29009829777031887No Hit
GTACCAGGGATGTGGTCATAGTTGACTGCTGAGAAAAGGACAATGAAATGAT1170.28050827139774637No Hit
AACTGTGGGGATATTAATCATCTTTGAGTAATTCTTCTAATCAAACATGCTC1160.2781107648046032No Hit
CTAAGTGGGATGCCTATATTCCCAATTATATTTTTCTAACTACTTCAGGACA1140.27331575161831695No Hit
TGCAGCGGGCAACATGATGCAGTTATCTTGCATACAACTGAAGACAACTAAC1120.26852073843203067No Hit
TTGTAGGGGGCGCACGCGCAGTGACGCGCCGGCCATGCCGGCGGCTGTTGTC1100.26372572524574445No Hit
CGGGCTGGGGTGGTTGAGATGGAAACCTTTCTCGCAAGCTTATTGAATATTT1090.2613282186526013No Hit
TAACAGGGGTAGGTAAATAATGGATTCCTATAATGGGAGCATCACCACTTAT1090.2613282186526013No Hit
AATGCTGGGATATAATGTATTTCTAGGTTCTGTGTTATGTTTTTTGTTGAAT1080.25893071205945817No Hit
AGTTGCGGGAGCAAAAGCACAGAGACTTAAGACTACTGAGTTGTGAAAATTA1070.25653320546631503No Hit
CGAATTGGGGAGTAACATAGGAGACCAAGAGGAAGTCAAAAGCTTGCTCCAA1050.25173819228002875No Hit
TAGCTTGGGCATTTAAATATTAATCAACTTTCCTTAAAATAAATAGCCTCTT1050.25173819228002875No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1040.24934068568688564No Hit
TACTGGGGGAGCAAATCCAAAGCAGAACTTAATGTCTCATATTAGAAGAATA1040.24934068568688564No Hit
CAAACAGAGGGACCGGCTGGAGCCACGGCAGAGAAAACATAAATTGTGAAGA1030.24694317909374253No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCC1020.2445456725005994No Hit
AAGCAGGGGGCCTCCTCCAGCCCCTCTCATGGCACCAATGGAAGTGTCCTCC1020.2445456725005994No Hit
ATATATGGGATAATAATCATTTCAGAATTTTACTCTGCATCACAATGTATTT1020.2445456725005994No Hit
AACTCCGGGAACTGGGTAAAAAGAACACAGGATCTCTGTATTATTTCTTACA1020.2445456725005994No Hit
TTTGATGGGCACCACAAAGAAGTACCTTGTCCCAAGTGTGTCCGGGCCTGAT1000.2397506593143131No Hit
CTACGGGGGGTCGCGGGGCAGACTAATCCCCTGCTCCTGGCCAGGGGAGGCT980.23495564612802683No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCT980.23495564612802683No Hit
AGCTGCGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTGT980.23495564612802683No Hit
ACTTTCGGGCTAAGAATGCTTTGCCATAACCACATTTAATGGGAAAAACGGC980.23495564612802683No Hit
TAATTGGGGGCTTTTCATTTGTCCAAGATGAGTACATAGCAGAATTTGGAAA970.23255813953488372No Hit
TGCTTTGGGAGTGCCCCAGGGGCCTCCTGGGCCACGGGCTGGAGGGGCTGCC950.22776312634859744No Hit
CTCAGTGGGGGCAGGAAGTTAACCTATTTAACAATTAGAGCTAGCATTTCAT930.2229681131623112No Hit
ATATCCGGGTCCATAAACCAAGTTAAAGTATGGCCCGACCATTTAAGAAAAC930.2229681131623112No Hit
AGTACCGGGAGGAGAAGCACCGGGGCCTCACCGCCCCCTTTCTGCAGCCCTT920.22057060656916808No Hit
TCACTAGGGATCTCTGGACTGCAATGGTCTTCTTGTGCTAGAACTCAGGCTC910.2181730999760249No Hit
CGTTGCGGGGCTCTGCGTGTGCAGCTCTCTGCGTCCCTCCTGGGCCCTCCAG910.2181730999760249No Hit
TCACGCGGGCAGTGATGAGCACTGCACCTAACAACAGCAGAATGTTCTTCTC910.2181730999760249No Hit
GTAATTGGGGAGTTCAGAGCCTTTAAAAACATGATGTCTTCAGCAGAAAACA890.21337808678973869No Hit
AAACGCGGGAGACAGGACCAACTTCAGATTCCCAAAGAAGCCAGCTACAGAG890.21337808678973869No Hit
GCAACAGGAAGGGAAAGGACAGGAAATGGAATTCTTACAGGGAAGTAAGTAA880.21098058019659552No Hit
GTTTACGGGAGTTATAGTTACGAGAAAGATATGGTACAGGGAGGAAAATACT860.2061855670103093No Hit
TTGAGCGGGGAGCCCTGCTGCTGGGGTTGATTTTGAACTGGAGTCATTTTCT860.2061855670103093No Hit
ACTTTTGGGGTCCATGCCTGCCTTTTGTTGTTTACATGCTCTTCTGCCCAGA860.2061855670103093No Hit
GCCAGCGGGAGTCTATTTCATGGGATGAGAATGGAACTTGCATAGTGATTAA850.20378806041716616No Hit
GATGTAGGGATGACCAAGCAAAAAGACTTTAAAAAATGGTAATGAAAATGGA840.201390553824023No Hit
ATACAGGGAGACAAGCCAGTGAGCTCTCCCCTTGGGATCACTGGGGTGATCA830.19899304723087988No Hit
AACGATGGGGCAAATATTAAGATATGAAAGGATGATAAAATAAGTATTTCTG830.19899304723087988No Hit
TTATCTGGGATTTATTTTAAAAAAAAGAGAGAGTAACTACAGAAGAACTTTA830.19899304723087988No Hit
CATGGCGGGACCGCGCGGCGGCCGCTGCGGCGCGATGGCGGCGGGGCTGGCG800.1918005274514505No Hit
TGAACAGGGCGCGCTTTGTCCTTAGCGCCTGTCTGCTCTCCTCTAACTAGGA800.1918005274514505No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT790.18940302085830737No Hit
CACTGCGGGCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGTGCCCGCC790.18940302085830737No Hit
GCGACTGGGTTAATAGAACGTGATTAGTCATCGCCGAGAAGAAAGCATATTA780.18700551426516424No Hit
AAGTATGGGGTGCTCCTAGGGTTCTCCAGGTGGTTCTTCAGACCTTCACCTG770.1846080076720211No Hit
CTACAAGGGCGATCTTTTGAAGGGCAATGATGAAAAGCCCGACTGTACAAAC760.18221050107887796No Hit
TATGTGGGGATGCAGCAAAGGTGCTGTGAGCCCAGTTCCAACGGCTGTGTTC760.18221050107887796No Hit
GGGAACGGGGCTCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCA750.17981299448573482No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTC750.17981299448573482No Hit
CCATCACGGGGGGCTCACTGCTATCTCCGCCTCCTGGGTTCCAGTGATTCTC750.17981299448573482No Hit
GCAATAGGGGAAATACCACTTGTGTCCCTCCTCAAGTTCTCTGACCACCTTC750.17981299448573482No Hit
TGGCTGGGGAGTGTCTATTTCCTGAGGAACCCAACTTATAACACGTAGAAGA740.1774154878925917No Hit
TCGCCGGGGTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGG730.17501798129944857No Hit
CGTAGTGGGCTGCTAGAAAACACATTGTACTGTGCTTTTTCTGGAATTCAGT730.17501798129944857No Hit
CGTCACGGGCCAGCCTTACTGGGTCCTTACCCTGGGCCAAACAGGGAGGGCT710.17022296811316232No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAAT710.17022296811316232No Hit
TGCGAACGGGAATTATTTCTTGCTAAATTCCAAATATTTGAAATTTGAAGAA710.17022296811316232No Hit
TATGCAGGGATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAG710.17022296811316232No Hit
CTGATTGGGGGGTTAAAAATATCTTTTCTATGTTGGTGTAAACTTTTTCGAA700.1678254615200192No Hit
TAAAATGGGGGTCCCTCCTTAAATGTCCGGCGGGGAGGGGCCTGTGACCTGA690.16542795492687606No Hit
TCAGCTGGGAAGCATGGCCCTGCTGCCCCTCCCTGCCTCCACCCACAGTGGA690.16542795492687606No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTC680.1630304483337329No Hit
GCAACAGGGAGGAGCGCAGGGACGGCAGGGCCAAGCCCGAGGAGGTGCACCG660.15823543514744665No Hit
CTAAAAGGGAGGCATTAAAAGAACAAGTTTCCAATGCAGACTGGTGTTTGGG660.15823543514744665No Hit
GATTTAGGGGCGGCCAGTAGAGGGATTCTGGGTAACGGCCCGGACCCCCGGC660.15823543514744665No Hit
CTTTACACGGGGAGACTGGGGTGGAGACTTCGGAGACTGCAGTTGCAGTTGT640.1534404219611604No Hit
GCTAATTTTTTTGTATTTTTAGTAGAGACAGAGTTTCACTGTGTTAGCCAGG640.1534404219611604No Hit
ATGATAGGGCAGTCACCGGCTTCGTCCTTAGTTTTCCCAACTTGGGACGTGA640.1534404219611604No Hit
ATAACTGGGAGAGCCTTCTCCACCGCCGGGGAATATTCATGTGGGAAATGGA640.1534404219611604No Hit
ATGCGGGCTAGACCTTGATCAACTCACTTTAAACTGGTCTCTGATTCCTCAT640.1534404219611604No Hit
AAGCATGGGGTGCCCAGTGCTTAGCTCATAAAAGATGCTAAATTAATGTTAT640.1534404219611604No Hit
GCTAACGGGTGCTGCTTCGGGGCTTAGGCCACCGCTCCCTGTCCCCAGAGGA640.1534404219611604No Hit
ATAGTGGGGTACTCACCGAAGCTGTGGCCCAGAATGGAGAATCGATTCCATT630.15104291536801726No Hit
TTCCGTGGGGGACGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGC620.14864540877487414No Hit
CTTATTGGGGCTCTTTCTCGACTCCATCTTCGCGGTAGCTGGGACCGCCGTT620.14864540877487414No Hit
TGTAAGGGGATGGCAGCCCGGCTGCTGCTTGGATGCGAGCTTGGCCTTGGGC610.146247902181731No Hit
AGCGCTGGGAACATTAATGTACTGTGAATCGTTCCTGATAAGTGAATAAAAC610.146247902181731No Hit
GTACTAGGGCTAGGATGTAACAGTGAAGCTGAGTAAACTATAACTGTTAAAC600.14385039558858786No Hit
TGACGCGGGTAATTGTCAGATTCCACTACCCTTTCTTCCTCATAGATAGTAA600.14385039558858786No Hit
ATCATGTGGGTCTGGAAAAGGGATGTCAGTAGAATCAAGGCAGTTTAAGGTT600.14385039558858786No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCG590.14145288899544473No Hit
TTAACACGGGATGTCTGTTGTATTTTCAGTAATGTGATTTCAGATGGTCATC590.14145288899544473No Hit
AACTAGGGGGCTGGTGAATAATTTTTTTGTCTTGCTTAGTGGCAAAGTGGAT590.14145288899544473No Hit
TCTGTGTGGGCAAATCTGTCAAGGTTATCAGAAGCAAGCAAAGTCTGAGAAA580.1390553824023016No Hit
TTGACCGGGGTTCCCCCAGTACCTCCAAGCAAGTAGCTTTCCACATTTGTCA580.1390553824023016No Hit
TACAAGGGGGTTAGGGTGTTAGTGATAAGTATGCATAAATGTCAAAGATGGG570.13665787580915847No Hit
TTTTATGGGAACACATCTGCTTCCAAAACTTTTTAAAAAGCTTTTAGTGTGG570.13665787580915847No Hit
ATCACCGGGTAGGGAGCCCTCCCATTGCCTTCCTGGCCCGGGATAGAAGAGG560.13426036921601534No Hit
AGTCCAGGGGAGGACGGGGCGGAGCTTCCGCCTGGGCTGGGGTTGGCGAGTT560.13426036921601534No Hit
GTTTCTGGGTACTGAACTTGGAATCACAGGACTGGGGAATATTCATTGGGGA550.13186286262287222No Hit
TTCACTGGGCTCATAGATAGTAATTACCAATGTAACTAAGTATTTGTGTTCT540.12946535602972908No Hit
CTCACTTGGGGGGGGTGGGCTTGGCCGAGCTGGCCTCCGGGGCACCGACCGC540.12946535602972908No Hit
TAGCTGGGGCCATTCTGTTATTCTTATGTATAACCACCTTTTAAAAATATTA540.12946535602972908No Hit
TATGTTGGGGTATATCTGGTGAAAATATCCTTCAAACATGAAGAAGTAGTAA530.12706784943658594No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACCTCGTATGC520.12467034284344282No Hit
GAGCAAGGGAAGCCAGGCTGGGCCTGGGCATAACTTCGCTGGGCTCTCCCAA510.1222728362502997No Hit
TTAGTGGGGCAACACCAGACAGCCATATTATGAAGAAATTAAGTGGAAATTA500.11987532965715655No Hit
TAGCAAGGGCAGTGATCATTAAGACAGGTAATTTAGGTGAAAGCATTTTAGA500.11987532965715655No Hit
CATCACGGGTCTAAGGAAGGGGAGAGGTGAAGTAAAACCGATACCTGTCCTG490.11747782306401341No Hit
TGCGTTGGGGGACCACCAAGGGGTTGTTGAAAACTAATTGTATTAAATGTCT490.11747782306401341No Hit
GCGCATGGGCGCTATAATGCTCTGCTGGCCGTGCAGAAGCTCATGGTGCACA490.11747782306401341No Hit
TGCTCCTGGGAGGTGCTATTTAAGAGTAAACCAAAGGATAAGCAGAGGGAGT490.11747782306401341No Hit
GGTAACGGGGACAGCTCTTTGGGCGTTGATTCTAGGGTCAGGGCCCTCACTC480.1150803164708703No Hit
TTCTACGGGACTGTTTTTTTGTGAGAATTGTCAGTGTAACTATTACCTACCA480.1150803164708703No Hit
ATTATTGGGCAAGCTGGGAAGGCCAAGAGCCTTCCTTCAGCCTTTCTGGTTA480.1150803164708703No Hit
AGTAGCGGGATGGGAGATTTCTAAATCTATTTGTCTCAGTGAAATAAGAGGG480.1150803164708703No Hit
AGAATCGGGGTCCATGAGGAGAAAAAAAGCATGTGTCATGCATGAAGGAAAC480.1150803164708703No Hit
CTTAGTTGGGAGATTTCAGTTATTGAATACTTATTCTGTGGCAGTCACTCTT460.11028530328458404No Hit
TAATGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC460.11028530328458404No Hit
AAGTCGGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGGTGAGTG460.11028530328458404No Hit
ATTCAAGGGTGAAAGAAGCTGTGCTGTACGTGGATTCCCAGGAGGCTGCCCT450.1078877966914409No Hit
GAAATTGGGGGAGGAAGGAAAGGTCGCAGCCTTCAAGGAGGAGCACATCTAC440.10549029009829776No Hit
TGTAAGGGGACGCTCTTCCGATCTTATCATGGGTTGTATGTTAAAGTGACTG440.10549029009829776No Hit
GTACTAGGGATGACACATTCTGAAATGAGAAAAGAACAGAGCCATGAGGACC430.10309278350515465No Hit
GTTAATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGA420.1006952769120115No Hit
ACTTGAGGGACGTGTTTCTGAAGTGGTTTAATCGTATTTGTTTAGCTTCTTT420.1006952769120115No Hit
TAATAAGGGCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACA420.1006952769120115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)