FastQCFastQC Report
Sat 31 Dec 2022
SRR5846339.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846339.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41250
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC20244.906666666666667No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT15503.7575757575757573No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC6881.667878787878788No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC4181.0133333333333332No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA4110.9963636363636363No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA3780.9163636363636364No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT1910.46303030303030307No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC1640.3975757575757576No Hit
GTACCAGGGATGTGGTCATAGTTGACTGCTGAGAAAAGGACAATGAAATGAT1240.3006060606060606No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1170.28363636363636363No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGT1160.2812121212121212No Hit
GAGATAGGGCAAGAGGACAGTGATGGTCACTTAAGTGTGAATTTGGAGTATG1150.2787878787878788No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCT1150.2787878787878788No Hit
AACTGTGGGGATATTAATCATCTTTGAGTAATTCTTCTAATCAAACATGCTC1130.2739393939393939No Hit
CTCAGTGGGGGCAGGAAGTTAACCTATTTAACAATTAGAGCTAGCATTTCAT1070.2593939393939394No Hit
TTGTAGGGGGCGCACGCGCAGTGACGCGCCGGCCATGCCGGCGGCTGTTGTC1060.25696969696969696No Hit
GCGCCCGGGTCTATTGGAGGCGCCCCATCTCAGACTTCCTAACACAGCCTGT1060.25696969696969696No Hit
ATGATAGGGCAGTCACCGGCTTCGTCCTTAGTTTTCCCAACTTGGGACGTGA1050.2545454545454546No Hit
TCACGCGGGCAGTGATGAGCACTGCACCTAACAACAGCAGAATGTTCTTCTC1050.2545454545454546No Hit
TACTGGGGGAGCAAATCCAAAGCAGAACTTAATGTCTCATATTAGAAGAATA1040.25212121212121213No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCC1010.24484848484848487No Hit
TTATGAGGGACCTGGGTGTCTGGGCTCAAGCTTTCCCGCCCAGCCTCACTTC1010.24484848484848487No Hit
AATGCTGGGATATAATGTATTTCTAGGTTCTGTGTTATGTTTTTTGTTGAAT1000.24242424242424243No Hit
TAACAGGGGTAGGTAAATAATGGATTCCTATAATGGGAGCATCACCACTTAT1000.24242424242424243No Hit
TTTGATGGGCACCACAAAGAAGTACCTTGTCCCAAGTGTGTCCGGGCCTGAT990.24No Hit
CTACAAGGGCGATCTTTTGAAGGGCAATGATGAAAAGCCCGACTGTACAAAC970.23515151515151514No Hit
ACTTTTGGGGTCCATGCCTGCCTTTTGTTGTTTACATGCTCTTCTGCCCAGA970.23515151515151514No Hit
CAAACAGAGGGACCGGCTGGAGCCACGGCAGAGAAAACATAAATTGTGAAGA960.23272727272727273No Hit
CTAAGTGGGATGCCTATATTCCCAATTATATTTTTCTAACTACTTCAGGACA950.2303030303030303No Hit
CTACGGGGGGTCGCGGGGCAGACTAATCCCCTGCTCCTGGCCAGGGGAGGCT940.22787878787878788No Hit
GCAACAGGAAGGGAAAGGACAGGAAATGGAATTCTTACAGGGAAGTAAGTAA940.22787878787878788No Hit
CGTAGTGGGCTGCTAGAAAACACATTGTACTGTGCTTTTTCTGGAATTCAGT940.22787878787878788No Hit
AACTCCGGGAACTGGGTAAAAAGAACACAGGATCTCTGTATTATTTCTTACA940.22787878787878788No Hit
TCACTAGGGATCTCTGGACTGCAATGGTCTTCTTGTGCTAGAACTCAGGCTC920.22303030303030302No Hit
ACTTTCGGGCTAAGAATGCTTTGCCATAACCACATTTAATGGGAAAAACGGC920.22303030303030302No Hit
AAACGCGGGAGACAGGACCAACTTCAGATTCCCAAAGAAGCCAGCTACAGAG910.22060606060606058No Hit
TAGCTTGGGCATTTAAATATTAATCAACTTTCCTTAAAATAAATAGCCTCTT910.22060606060606058No Hit
ATACAGGGAGACAAGCCAGTGAGCTCTCCCCTTGGGATCACTGGGGTGATCA900.2181818181818182No Hit
CGGGCTGGGGTGGTTGAGATGGAAACCTTTCTCGCAAGCTTATTGAATATTT900.2181818181818182No Hit
GTAATTGGGGAGTTCAGAGCCTTTAAAAACATGATGTCTTCAGCAGAAAACA880.21333333333333335No Hit
GTTTACGGGAGTTATAGTTACGAGAAAGATATGGTACAGGGAGGAAAATACT870.2109090909090909No Hit
ATATATGGGATAATAATCATTTCAGAATTTTACTCTGCATCACAATGTATTT870.2109090909090909No Hit
CGTTGCGGGGCTCTGCGTGTGCAGCTCTCTGCGTCCCTCCTGGGCCCTCCAG860.2084848484848485No Hit
AGTTGCGGGAGCAAAAGCACAGAGACTTAAGACTACTGAGTTGTGAAAATTA860.2084848484848485No Hit
GGGAACGGGGCTCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCA850.20606060606060606No Hit
AGTACCGGGAGGAGAAGCACCGGGGCCTCACCGCCCCCTTTCTGCAGCCCTT850.20606060606060606No Hit
AAGCAGGGGGCCTCCTCCAGCCCCTCTCATGGCACCAATGGAAGTGTCCTCC830.2012121212121212No Hit
ATGCGGGCTAGACCTTGATCAACTCACTTTAAACTGGTCTCTGATTCCTCAT830.2012121212121212No Hit
TATGCAGGGATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAG830.2012121212121212No Hit
TAATTGGGGGCTTTTCATTTGTCCAAGATGAGTACATAGCAGAATTTGGAAA830.2012121212121212No Hit
CGAATTGGGGAGTAACATAGGAGACCAAGAGGAAGTCAAAAGCTTGCTCCAA820.1987878787878788No Hit
GATGTAGGGATGACCAAGCAAAAAGACTTTAAAAAATGGTAATGAAAATGGA820.1987878787878788No Hit
TGAACAGGGCGCGCTTTGTCCTTAGCGCCTGTCTGCTCTCCTCTAACTAGGA810.19636363636363635No Hit
TTCTACGGGACTGTTTTTTTGTGAGAATTGTCAGTGTAACTATTACCTACCA800.19393939393939394No Hit
CGTCACGGGCCAGCCTTACTGGGTCCTTACCCTGGGCCAAACAGGGAGGGCT780.1890909090909091No Hit
TTAACACGGGATGTCTGTTGTATTTTCAGTAATGTGATTTCAGATGGTCATC780.1890909090909091No Hit
AAGTATGGGGTGCTCCTAGGGTTCTCCAGGTGGTTCTTCAGACCTTCACCTG780.1890909090909091No Hit
TAGCTGGGGCCATTCTGTTATTCTTATGTATAACCACCTTTTAAAAATATTA770.18666666666666668No Hit
GCGACTGGGTTAATAGAACGTGATTAGTCATCGCCGAGAAGAAAGCATATTA770.18666666666666668No Hit
AACGATGGGGCAAATATTAAGATATGAAAGGATGATAAAATAAGTATTTCTG740.17939393939393938No Hit
TGTAAGGGGATGGCAGCCCGGCTGCTGCTTGGATGCGAGCTTGGCCTTGGGC740.17939393939393938No Hit
TTGAGCGGGGAGCCCTGCTGCTGGGGTTGATTTTGAACTGGAGTCATTTTCT740.17939393939393938No Hit
AGCTGCGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTGT740.17939393939393938No Hit
TATGTGGGGATGCAGCAAAGGTGCTGTGAGCCCAGTTCCAACGGCTGTGTTC740.17939393939393938No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTC730.17696969696969697No Hit
ATAACTGGGAGAGCCTTCTCCACCGCCGGGGAATATTCATGTGGGAAATGGA730.17696969696969697No Hit
GCTAATTTTTTTGTATTTTTAGTAGAGACAGAGTTTCACTGTGTTAGCCAGG720.17454545454545456No Hit
CATGGCGGGACCGCGCGGCGGCCGCTGCGGCGCGATGGCGGCGGGGCTGGCG720.17454545454545456No Hit
AACTAGGGGGCTGGTGAATAATTTTTTTGTCTTGCTTAGTGGCAAAGTGGAT700.1696969696969697No Hit
GCCAGCGGGAGTCTATTTCATGGGATGAGAATGGAACTTGCATAGTGATTAA690.16727272727272727No Hit
CCATCACGGGGGGCTCACTGCTATCTCCGCCTCCTGGGTTCCAGTGATTCTC690.16727272727272727No Hit
GCAATAGGGGAAATACCACTTGTGTCCCTCCTCAAGTTCTCTGACCACCTTC690.16727272727272727No Hit
TTTTATGGGAACACATCTGCTTCCAAAACTTTTTAAAAAGCTTTTAGTGTGG690.16727272727272727No Hit
AAGCATGGGGTGCCCAGTGCTTAGCTCATAAAAGATGCTAAATTAATGTTAT690.16727272727272727No Hit
TGGCTGGGGAGTGTCTATTTCCTGAGGAACCCAACTTATAACACGTAGAAGA680.16484848484848486No Hit
TCTGTGTGGGCAAATCTGTCAAGGTTATCAGAAGCAAGCAAAGTCTGAGAAA680.16484848484848486No Hit
TGCTTTGGGAGTGCCCCAGGGGCCTCCTGGGCCACGGGCTGGAGGGGCTGCC680.16484848484848486No Hit
TGTAAGGGGACGCTCTTCCGATCTTATCATGGGTTGTATGTTAAAGTGACTG680.16484848484848486No Hit
ATATCCGGGTCCATAAACCAAGTTAAAGTATGGCCCGACCATTTAAGAAAAC680.16484848484848486No Hit
AGCGCTGGGAACATTAATGTACTGTGAATCGTTCCTGATAAGTGAATAAAAC680.16484848484848486No Hit
TTCCGTGGGGGACGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGC670.16242424242424244No Hit
TCAGCTGGGAAGCATGGCCCTGCTGCCCCTCCCTGCCTCCACCCACAGTGGA670.16242424242424244No Hit
GCAACAGGGAGGAGCGCAGGGACGGCAGGGCCAAGCCCGAGGAGGTGCACCG660.16No Hit
TAAAATGGGGGTCCCTCCTTAAATGTCCGGCGGGGAGGGGCCTGTGACCTGA650.1575757575757576No Hit
CTGATTGGGGGGTTAAAAATATCTTTTCTATGTTGGTGTAAACTTTTTCGAA650.1575757575757576No Hit
TGCGAACGGGAATTATTTCTTGCTAAATTCCAAATATTTGAAATTTGAAGAA650.1575757575757576No Hit
TGCAGCGGGCAACATGATGCAGTTATCTTGCATACAACTGAAGACAACTAAC650.1575757575757576No Hit
ATTCAAGGGTGAAAGAAGCTGTGCTGTACGTGGATTCCCAGGAGGCTGCCCT640.15515151515151515No Hit
CTAAAAGGGAGGCATTAAAAGAACAAGTTTCCAATGCAGACTGGTGTTTGGG640.15515151515151515No Hit
CTTTACACGGGGAGACTGGGGTGGAGACTTCGGAGACTGCAGTTGCAGTTGT630.15272727272727274No Hit
CACTGCGGGCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGTGCCCGCC630.15272727272727274No Hit
CTTATTGGGGCTCTTTCTCGACTCCATCTTCGCGGTAGCTGGGACCGCCGTT630.15272727272727274No Hit
TCGCCGGGGTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGG620.1503030303030303No Hit
GAAATTGGGGGAGGAAGGAAAGGTCGCAGCCTTCAAGGAGGAGCACATCTAC610.14787878787878786No Hit
AGTCCAGGGGAGGACGGGGCGGAGCTTCCGCCTGGGCTGGGGTTGGCGAGTT610.14787878787878786No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT600.14545454545454545No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATC600.14545454545454545No Hit
GTTTCTGGGTACTGAACTTGGAATCACAGGACTGGGGAATATTCATTGGGGA590.14303030303030304No Hit
ATTGCCGGGGGTGTTCCTCATATTATGGTACAGGGCCAAAGACTTGAGACGT580.1406060606060606No Hit
TTATCTGGGATTTATTTTAAAAAAAAGAGAGAGTAACTACAGAAGAACTTTA580.1406060606060606No Hit
ATCATGTGGGTCTGGAAAAGGGATGTCAGTAGAATCAAGGCAGTTTAAGGTT580.1406060606060606No Hit
GATTTAGGGGCGGCCAGTAGAGGGATTCTGGGTAACGGCCCGGACCCCCGGC550.13333333333333333No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACCTCGTATGC540.13090909090909092No Hit
ATTAATGGGGGTTGAGGACATAAGACATTGTTATAGAAACTAGGTCAACTTC540.13090909090909092No Hit
TATGTTGGGGTATATCTGGTGAAAATATCCTTCAAACATGAAGAAGTAGTAA520.12606060606060607No Hit
CTCACTTGGGGGGGGTGGGCTTGGCCGAGCTGGCCTCCGGGGCACCGACCGC520.12606060606060607No Hit
ACTTGAGGGACGTGTTTCTGAAGTGGTTTAATCGTATTTGTTTAGCTTCTTT500.12121212121212122No Hit
TTCACTGGGCTCATAGATAGTAATTACCAATGTAACTAAGTATTTGTGTTCT500.12121212121212122No Hit
AAGTCGGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGGTGAGTG500.12121212121212122No Hit
TGCGTTGGGGGACCACCAAGGGGTTGTTGAAAACTAATTGTATTAAATGTCT490.11878787878787879No Hit
GAATCGGGGAGTTCATATTAGAATAAATTGCCCAATCTATAAGAGAGTGTTT490.11878787878787879No Hit
TAGCAAGGGCAGTGATCATTAAGACAGGTAATTTAGGTGAAAGCATTTTAGA490.11878787878787879No Hit
TACGCAGGGTGAGTTTCTTCTTCCCCTTGAGCTTCAGAGAGGAGAGTTGGCA480.11636363636363636No Hit
GTTGCCCTGTTAGATCTTTACTGAGTGAATTATAAATGTGTGTTAAATACTT480.11636363636363636No Hit
GCTAACGGGTGCTGCTTCGGGGCTTAGGCCACCGCTCCCTGTCCCCAGAGGA480.11636363636363636No Hit
ATCACCGGGTAGGGAGCCCTCCCATTGCCTTCCTGGCCCGGGATAGAAGAGG470.11393939393939394No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCG470.11393939393939394No Hit
GGTAACGGGGACAGCTCTTTGGGCGTTGATTCTAGGGTCAGGGCCCTCACTC460.11151515151515151No Hit
CTCGCTGGGGTTTGCTTTTGACCATCACCTCCCAGTAGCAATTTGCTTTCAT460.11151515151515151No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAAT460.11151515151515151No Hit
GTTAATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGA460.11151515151515151No Hit
ATTATTGGGCAAGCTGGGAAGGCCAAGAGCCTTCCTTCAGCCTTTCTGGTTA460.11151515151515151No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTC460.11151515151515151No Hit
CGTCATGGGAGCTGTGTACTTCTATGTATTTAATAAACTACTGCCTCATTTC460.11151515151515151No Hit
GTACTAGGGCTAGGATGTAACAGTGAAGCTGAGTAAACTATAACTGTTAAAC450.1090909090909091No Hit
GTTGTAGGGCAGTGCCCCAGCTGGGAAGAGGGCGGGATCGGGCACTGGTTCC450.1090909090909091No Hit
AAGCCTGGGCAACAAGAGCAAAACTCCATCTCAAAAAAAAAAAAAAAAAAAA450.1090909090909091No Hit
TGACGCGGGTAATTGTCAGATTCCACTACCCTTTCTTCCTCATAGATAGTAA450.1090909090909091No Hit
TTAGTGGGGCAACACCAGACAGCCATATTATGAAGAAATTAAGTGGAAATTA450.1090909090909091No Hit
GCGTGAGGGATGGAAATAAGTGTTAAGAAAAGGATTGCTTATTGGTAGCATA440.10666666666666667No Hit
AGTAGCGGGATGGGAGATTTCTAAATCTATTTGTCTCAGTGAAATAAGAGGG440.10666666666666667No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATG430.10424242424242425No Hit
TGCTCCTGGGAGGTGCTATTTAAGAGTAAACCAAAGGATAAGCAGAGGGAGT430.10424242424242425No Hit
GCTAAGGGATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTG430.10424242424242425No Hit
TACAAGGGGGTTAGGGTGTTAGTGATAAGTATGCATAAATGTCAAAGATGGG420.10181818181818182No Hit
ATAGTGGGGTACTCACCGAAGCTGTGGCCCAGAATGGAGAATCGATTCCATT420.10181818181818182No Hit
TTACTAGGGATAGCAAGTGTACAGGAAATTTATTCACTCTTATTTATTGATT420.10181818181818182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)