FastQCFastQC Report
Sat 31 Dec 2022
SRR5846333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159098
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT28021.7611786446089832No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC26751.6813536310952997No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC7360.4626079523312675No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC6360.39975361098191053No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA5120.3218142277087078No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA4500.2828445360721065No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC2930.184163220153616No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT1930.12130887880425902No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1840.11565198808281688No Hit
CTTAGCGGGGAAGAAAGGCATGGAGGCTAGAAATAAAGTAGATAAAGTTATT1620.10182403298595834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)