FastQCFastQC Report
Sat 31 Dec 2022
SRR5846327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163252
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT31041.901354960429275No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC29831.827236419768211No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC7920.4851395388724181No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC7570.46370029157376325No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA5650.346090706392571No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA4630.28361061426506257No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC2720.16661357900668905No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT2160.1323107833288413No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1810.11087153603018647No Hit
GGATGGGGGAGAATTCGTAGCACTTTCATGTGAAAGCAGACCCAGTCATTAT1760.10780878641609291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)