FastQCFastQC Report
Sat 31 Dec 2022
SRR5846326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99356
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT37723.796449132412738No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC35323.554893514231652No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC9490.9551511735577117No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC8250.8303474374974837No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA7610.7659326059825275No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA6040.6079149724224002No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC2720.2737630339385644No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT2400.2415556181810862No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1920.19324449454486894No Hit
GCACGAGGGCCTCTTCCCAGAGGTGTCGGGGCTTGGCCCCAGCCTCCATCTT1910.19223801280244776No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCT1850.18619912234792063No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGT1680.16908893272676034No Hit
TAATCAGGGGATCCGGGTCTCTCGCGCGAGCGATTTAGTCTGAGGCGAAGCT1650.16606948749949677No Hit
TACGGGAGGGAACATTTCTGTTGCTCCACATCCTTGCTGGCACTTGGTATTG1550.15600467007528485No Hit
TCTCCTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1540.15499818833286363No Hit
AGTTAAGGGGAAATTCATTTTAGTCAACTTGATTAAAATGCTCAAAGGATTA1480.14895929787833648No Hit
CGCTCGGGCCGATCTGAAGCAGGGAGGGGCTTAAGCGGAGGGAGTCGAGCCA1430.14392688916623053No Hit
CGCCGAGGGGGATGATAGAATAATTTCCTTAGAAAACAGATTACAATAGTAT1410.14191392568138814No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCC1400.14090744393896695No Hit
CATACTGGGATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCG1390.13990096219654574No Hit
GGATTTGGGATCAAATCATATTGCCCAATTAATAGCATAGAATTGTATCTAC1380.13889448045412456No Hit
AATTGTGGGGATCAAAGCCAATCTTTGAACATCCACATTGCTGAGCCTAACT1370.13788799871170337No Hit
CGTATCGGGATATGTAGCTGATTTAATAAGCTAGTTAGTGAATGGAAAATAA1360.1368815169692822No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT1350.13587503522686098No Hit
TGATATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1340.1348685534844398No Hit
TTATGCGGGGAAAAGAGAGATTTTTGGAAAGCACCCAAATTTAAGAGAGTGC1330.13386207174201858No Hit
AAAGCAGGGAGGAGTTGTAGACTACCTAAATTTTTAAGTTATGGTATTTGTT1330.13386207174201858No Hit
CGCAACGGGCTGGTGGAGCTGGAGGGCCGCAGGGCCGCCCGGTGGGGTCAGG1330.13386207174201858No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACCTCGTATGC1310.13184910825717622No Hit
GTTAGTGGGGGGCATCCTGTGGTATAGGGGAAGCGCTCCGGGCCTGGAATCC1300.13084262651475503No Hit
AGTTCAGGGTCTGTCGTTGGGGGGGACAGAAAGTTGTGTAAAGCTTGAAGGT1290.12983614477233382No Hit
ACGTCCCGGGTGGACAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGTC1280.12882966302991264No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACCTCGTATGCCGTCT1280.12882966302991264No Hit
CATCTTGGGGGAGAAGGGTTTTTTTCAGGCTGCGGGTCGGAGGGCAGAAGTG1270.12782318128749143No Hit
GTTACCGGGAATGACTGGGAAACAACTATAGAAAACTTTCATGTGGTGGAAA1260.12681669954507027No Hit
ATATGTGGGATCTAATGTACTTTTACATATTTGTAAAAACAATTTTTGGTAA1250.12581021780264906No Hit
CGCTCCGGGAGTAACAGGTGGGTACCACTCCTTTTCCCCTCATGGTGCCCAT1250.12581021780264906No Hit
AGCTTAGGGAAGTCCACCTGAGTCTGAGATTCTCCATCTGTAAAAAAGGAAT1240.12480373606022788No Hit
TTAGCGGGGAGTATCTAATTATTATGTGATAGCCTCTTTTGGCTTTTTTAGT1240.12480373606022788No Hit
GCCCCTGGGGAATACAGGCAGAAGGATACAGCTTGATGCTAACACTGCATGA1210.12178429083296428No Hit
CGCAGGGGGACCTTAGTGGAAGATGCTCATCTTTTATTTATTTATTTTTCTA1190.1197713273481219No Hit
AGGCACGGGAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAATGCAG1170.11775836386327951No Hit
ATCTTAGGGCCCAGGCTCAGGGACTCTGGTGCCAAATCCAGAAAGATCTGCT1170.11775836386327951No Hit
GGATGCGGGAAGAACAGTAATGATAACCTAAGGCAGTTTAACAGTATGGAAT1170.11775836386327951No Hit
ATGCGCGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1160.11675188212085834No Hit
GGAAATGGGGTATTACTGCTGGGATTTGTTTTATTATTCAGACTTTAAGATC1130.11373243689359475No Hit
AGCAATGGGATAGAAGGGGAAGAAATTGAAATAGTCTTAGCCAAGCCACCAG1130.11373243689359475No Hit
CATTGTGGGATTACATGTAATTATGGCATGTAATCAGAATTATGGCATCTAA1110.11171947340875235No Hit
GCCAATGGGGGAAATAATTTACCAAAATAACTGAGTGTTGCTGCTAAAGAAC1110.11171947340875235No Hit
CGCAATGGGCAGCCCTACCCTGCATAATTTGAAGACACAGCAGAAAGGGGGC1110.11171947340875235No Hit
TTAATAGGGGTGCTGCAGGCTTGGAATCTTAATGAGGCCTGAGCCCTGACCG1100.11071299166633118No Hit
AAAATAGGGGTATATTGATATATACAAATATATATCAGTAGAATATTGACAA1100.11071299166633118No Hit
ATACGAGGGCACTTATACTTGGATTTGCTCTCTTCCCATTTCTGATTGTTGT1100.11071299166633118No Hit
GCATGAAGGGAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCC1090.10970650992390997No Hit
TAATTGGGATAATTTTATCATTTATGCAGAAAGGAAGAACATGTCACTGTCA1080.10870002818148879No Hit
TCATTTGGGATTGGTAGTTTATAGGAATAGCATTGAATCTGTAGATTGCTTT1080.10870002818148879No Hit
AGCCTAGGGGTGGATTTGTTGCTCTTTTTGTCCTTTTTTGAAGCTGTTATTA1070.10769354643906759No Hit
AATAGTGGGAAGACTTTTAAAATCAACTGATAATCACCATTATTGTAATAAA1070.10769354643906759No Hit
TGAATGGGGGAAGCTAGGTTTCAAGATAAACAATATAGCTCCCATCACACAG1060.1066870646966464No Hit
TGTTGTGGGATTATCACAGGGGAAACATAGAATTTTGCTTACCAAACGTTAA1050.10568058295422521No Hit
CGACTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1050.10568058295422521No Hit
GATTACGGGATGTTGAGGCAGGAAGTTTAGTAGAATTCCAAGGAAGTAAGAG1050.10568058295422521No Hit
GCTGTCGGGCTACAAAGATGCAAAGACTGCTGCTTCTGGAATTCCCTACCAC1050.10568058295422521No Hit
CAACCGGGACGGCATGACCTAGTAAGATTTATCCTAGGAATGCAAGGTTGTT1050.10568058295422521No Hit
GTTTGCGGGCATTCACACTCCCAAAGGCGAGGATTTACCCTAGAAATCATAA1050.10568058295422521No Hit
ATGCCGGGGCTGAGAGAACAATTTGATTGATTAATTGATCTCATGTTAGCAA1050.10568058295422521No Hit
TATAATGGGGCTGTTCACATTAAGCAGTTTATTAGGGGGTTATTTTGAAATC1030.10366761946938281No Hit
TCTAGGGATGTTGGCCAGGCTGGTTTTTAACTCCTGGCCTTACGTGATCCGC1020.10266113772696163No Hit
ATAGCTGGGACCAAAAATGATAGTCACTGAAGGAAACCAAATCATAATTTTG1020.10266113772696163No Hit
GCAATGGGGGGATTATATTTGAGAGTAAATGAAGTGTGTATCAGTAATGTCA1020.10266113772696163No Hit
TTGGAAGGGAACATACATGTGCAGAAATGTATTCAAATGAAAGGAAGCATAC1020.10266113772696163No Hit
TGCTTGGGGATTCCTGTTTTTATCCAGCAAGATGTAAGATGATTTAATATTT1020.10266113772696163No Hit
GTGCTGGGGATGCCAGATTGAACTTTGATTTTTACTACCTTTTCAAAGATAT1010.10165465598454043No Hit
ATCATGGGGGGCCCCTGCCACTGGGCCATGGAGACTGTGGCACAGTAGACTG1000.10064817424211925No Hit
TCGGTTGGGGGGACTTCTAGTACAGCCCAGAATGGATTTGGCATTATTTATG1000.10064817424211925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)