FastQCFastQC Report
Sat 31 Dec 2022
SRR5846325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97717
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT38003.8887808671981334No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC35783.6615941954828743No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC8840.9046532333166183No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC8300.8493916104669607No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA7320.7491019986286931No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA6840.699980556095664No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT2730.27937820440660277No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC2500.255840846526193No Hit
GCACGAGGGCCTCTTCCCAGAGGTGTCGGGGCTTGGCCCCAGCCTCCATCTT2140.21899976462642118No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1930.19750913351822097No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCT1870.19136895320159236No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCC1530.15657459807403012No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGT1470.1504344177574015No Hit
AGTTAAGGGGAAATTCATTTTAGTCAACTTGATTAAAATGCTCAAAGGATTA1460.1494110543712967No Hit
AGCTTAGGGAAGTCCACCTGAGTCTGAGATTCTCCATCTGTAAAAAAGGAAT1440.14736432759908716No Hit
TCTCCTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1430.14634096421298237No Hit
GTTAGTGGGGGGCATCCTGTGGTATAGGGGAAGCGCTCCGGGCCTGGAATCC1430.14634096421298237No Hit
TGAATGGGGGAAGCTAGGTTTCAAGATAAACAATATAGCTCCCATCACACAG1410.14429423744077283No Hit
CGCAACGGGCTGGTGGAGCTGGAGGGCCGCAGGGCCGCCCGGTGGGGTCAGG1410.14429423744077283No Hit
TAATCAGGGGATCCGGGTCTCTCGCGCGAGCGATTTAGTCTGAGGCGAAGCT1380.14122414728245852No Hit
CGCCGAGGGGGATGATAGAATAATTTCCTTAGAAAACAGATTACAATAGTAT1370.14020078389635376No Hit
ATCTTAGGGCCCAGGCTCAGGGACTCTGGTGCCAAATCCAGAAAGATCTGCT1370.14020078389635376No Hit
ATATGTGGGATCTAATGTACTTTTACATATTTGTAAAAACAATTTTTGGTAA1350.13815405712414422No Hit
TTATGCGGGGAAAAGAGAGATTTTTGGAAAGCACCCAAATTTAAGAGAGTGC1340.13713069373803943No Hit
AATTGTGGGGATCAAAGCCAATCTTTGAACATCCACATTGCTGAGCCTAACT1340.13713069373803943No Hit
GGATGCGGGAAGAACAGTAATGATAACCTAAGGCAGTTTAACAGTATGGAAT1340.13713069373803943No Hit
CGCTCGGGCCGATCTGAAGCAGGGAGGGGCTTAAGCGGAGGGAGTCGAGCCA1320.13508396696582992No Hit
ACGTCCCGGGTGGACAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGTC1300.13303724019362034No Hit
CATACTGGGATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCG1290.13201387680751558No Hit
GGATTTGGGATCAAATCATATTGCCCAATTAATAGCATAGAATTGTATCTAC1270.12996715003530604No Hit
AAAGCAGGGAGGAGTTGTAGACTACCTAAATTTTTAAGTTATGGTATTTGTT1270.12996715003530604No Hit
ATTAGTGGGATATAACACAATTAACAAATAATAAGATACAAATACTTTATTG1260.12894378664920128No Hit
TACGGGAGGGAACATTTCTGTTGCTCCACATCCTTGCTGGCACTTGGTATTG1260.12894378664920128No Hit
CGCAAGGGGCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGC1240.12689705987699174No Hit
AGTTCAGGGTCTGTCGTTGGGGGGGACAGAAAGTTGTGTAAAGCTTGAAGGT1190.12178024294646787No Hit
AGGACGGGTACCTAGGGGTGATTCATAAGAATCTTGTTTTCTCCGGGCTTCT1190.12178024294646787No Hit
GTGCTGGGGATGCCAGATTGAACTTTGATTTTTACTACCTTTTCAAAGATAT1180.1207568795603631No Hit
TTAGCGGGGAGTATCTAATTATTATGTGATAGCCTCTTTTGGCTTTTTTAGT1180.1207568795603631No Hit
GCATGAAGGGAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCC1170.11973351617425831No Hit
TTGGAAGGGAACATACATGTGCAGAAATGTATTCAAATGAAAGGAAGCATAC1170.11973351617425831No Hit
CATCTTGGGGGAGAAGGGTTTTTTTCAGGCTGCGGGTCGGAGGGCAGAAGTG1160.11871015278815354No Hit
AGCCTAGGGGTGGATTTGTTGCTCTTTTTGTCCTTTTTTGAAGCTGTTATTA1140.116663426015944No Hit
GCTCTGGGGAGACATAATATAGCAGGAACAACACATTCAGGTGCTGAAAAAG1140.116663426015944No Hit
GTATGTGGGATACTTGATTCACACAACAATGATGCCACAAGCTCCATACCCA1140.116663426015944No Hit
GCCAATGGGGGAAATAATTTACCAAAATAACTGAGTGTTGCTGCTAAAGAAC1130.11564006262983922No Hit
AGTTACGGGATACGTATGGGACCTACACTTAATCTATATGCTTTACACTAGC1120.11461669924373447No Hit
CGCAATGGGCAGCCCTACCCTGCATAATTTGAAGACACAGCAGAAAGGGGGC1110.1135933358576297No Hit
CATTGTGGGATTACATGTAATTATGGCATGTAATCAGAATTATGGCATCTAA1100.11256997247152492No Hit
AGTTGAGGGCACCATTGGTAACTTGTGTTATGAGTCTCCAATTTGGGGTTTT1100.11256997247152492No Hit
TGTGGTGGGCATAAATCTCGACCTATAAAAGAAAGAAGCAGCAGAACTTGAC1100.11256997247152492No Hit
GTTACCGGGAATGACTGGGAAACAACTATAGAAAACTTTCATGTGGTGGAAA1090.11154660908542013No Hit
CGCTCCGGGAGTAACAGGTGGGTACCACTCCTTTTCCCCTCATGGTGCCCAT1090.11154660908542013No Hit
AATTTAGGGGTTCAAGCTTTAGAAAGATCAGAGCAATTTTTCTCTTTCAGTC1080.11052324569931536No Hit
TGTTGTGGGATTATCACAGGGGAAACATAGAATTTTGCTTACCAAACGTTAA1080.11052324569931536No Hit
CCTAGCGGGATGGGCATAGAGGTAATTCTCTATTTCACTTTGGTGGAAGGAC1050.10745315554100106No Hit
CAACCGGGACGGCATGACCTAGTAAGATTTATCCTAGGAATGCAAGGTTGTT1050.10745315554100106No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT1040.10642979215489629No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACCTCGTATGC1040.10642979215489629No Hit
GTTTGCGGGCATTCACACTCCCAAAGGCGAGGATTTACCCTAGAAATCATAA1040.10642979215489629No Hit
GCCCCTGGGGAATACAGGCAGAAGGATACAGCTTGATGCTAACACTGCATGA1030.10540642876879151No Hit
GCAATGGGGGGATTATATTTGAGAGTAAATGAAGTGTGTATCAGTAATGTCA1030.10540642876879151No Hit
TGATATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1030.10540642876879151No Hit
GTTCAAGGGGGAAAAAACAGAGTGATGTTTTACTCAAATGCTTTCTAAGTAC1020.10438306538268674No Hit
CTAATCAGGGATCCAGAATCTTTAAGGAACTTAAGCAACTGAACAAGCAAAA1010.10335970199658195No Hit
ATGCGCGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1010.10335970199658195No Hit
AAAATAGGGGTATATTGATATATACAAATATATATCAGTAGAATATTGACAA1000.10233633861047718No Hit
AATAGTGGGAAGACTTTTAAAATCAACTGATAATCACCATTATTGTAATAAA1000.10233633861047718No Hit
AGTATTGGGAGATTTCTAGATACTTTTTGCTGCTAGTTTTATGTAATATTTA1000.10233633861047718No Hit
AGCAATGGGATAGAAGGGGAAGAAATTGAAATAGTCTTAGCCAAGCCACCAG1000.10233633861047718No Hit
TACAGGGGGATTGCATGCAAGTTTGCTGAGCTGAAGGAAAAGATTGATCGCC990.10131297522437244No Hit
GACACTGGGGGCTGTGGCACGAGGAAGCAAAGATCACAACATTCGTGCCCAA990.10131297522437244No Hit
AGGCACGGGAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAATGCAG990.10131297522437244No Hit
CTATTGGGGCCAGCAGGGGCACACTGATACCTCACACTGCAGGGTTCTCCAA980.10028961183826765No Hit
TCACGGGAGACGGGCTCAGTGTTGAGGATATTTTAAATCTTTTAGGGATGGA980.10028961183826765No Hit
GTCACACTGGGGAAAAAATTAAGCATATATTAAGTTTCTCCAGCATGAACTA980.10028961183826765No Hit
AGCTTGACGGGATGGGCAGGAAGCAAATGACCTGAAAACTAAATAAATCTAA980.10028961183826765No Hit
AATGCAGGGCCATAGTACTTTTTCATGAAAGAAATTTGCTAAATAAGACATG980.10028961183826765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)