FastQCFastQC Report
Sat 31 Dec 2022
SRR5846324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97673
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT36543.7410543343605704No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC34143.49533647988697No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC9110.9327040226060426No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC8100.8292977588484024No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA7220.7392012122080821No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA6620.6777717485896819No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC3370.345028820656681No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT2470.25288462522908073No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1830.1873598640361205No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGT1750.1791692688870005No Hit
GCACGAGGGCCTCTTCCCAGAGGTGTCGGGGCTTGGCCCCAGCCTCCATCTT1680.17200249813152046No Hit
ATATGTGGGATCTAATGTACTTTTACATATTTGTAAAAACAATTTTTGGTAA1590.16278807858876046No Hit
GTTAGTGGGGGGCATCCTGTGGTATAGGGGAAGCGCTCCGGGCCTGGAATCC1560.15971660540784044No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCC1550.15869278101420045No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCT1550.15869278101420045No Hit
TCTCCTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1480.15152601025872042No Hit
TAATCAGGGGATCCGGGTCTCTCGCGCGAGCGATTTAGTCTGAGGCGAAGCT1470.1505021858650804No Hit
TTAGCGGGGAGTATCTAATTATTATGTGATAGCCTCTTTTGGCTTTTTTAGT1460.14947836147144042No Hit
TACGGGAGGGAACATTTCTGTTGCTCCACATCCTTGCTGGCACTTGGTATTG1460.14947836147144042No Hit
AGTTAAGGGGAAATTCATTTTAGTCAACTTGATTAAAATGCTCAAAGGATTA1430.1464068882905204No Hit
CATTGTGGGATTACATGTAATTATGGCATGTAATCAGAATTATGGCATCTAA1410.1443592395032404No Hit
AGCTTAGGGAAGTCCACCTGAGTCTGAGATTCTCCATCTGTAAAAAAGGAAT1400.1433354151096004No Hit
TGAATGGGGGAAGCTAGGTTTCAAGATAAACAATATAGCTCCCATCACACAG1380.1412877663223204No Hit
TTGGAAGGGAACATACATGTGCAGAAATGTATTCAAATGAAAGGAAGCATAC1380.1412877663223204No Hit
CGCTCGGGCCGATCTGAAGCAGGGAGGGGCTTAAGCGGAGGGAGTCGAGCCA1360.13924011753504037No Hit
GGATTTGGGATCAAATCATATTGCCCAATTAATAGCATAGAATTGTATCTAC1360.13924011753504037No Hit
TGATATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1340.13719246874776037No Hit
ATGCCGGGGCTGAGAGAACAATTTGATTGATTAATTGATCTCATGTTAGCAA1330.13616864435412038No Hit
CGCCGAGGGGGATGATAGAATAATTTCCTTAGAAAACAGATTACAATAGTAT1310.13412099556684037No Hit
AGCCTAGGGGTGGATTTGTTGCTCTTTTTGTCCTTTTTTGAAGCTGTTATTA1310.13412099556684037No Hit
AGTTCAGGGTCTGTCGTTGGGGGGGACAGAAAGTTGTGTAAAGCTTGAAGGT1250.12797804920500036No Hit
CATACTGGGATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCG1240.12695422481136034No Hit
AAAGCAGGGAGGAGTTGTAGACTACCTAAATTTTTAAGTTATGGTATTTGTT1230.12593040041772036No Hit
CGTATCGGGATATGTAGCTGATTTAATAAGCTAGTTAGTGAATGGAAAATAA1210.12388275163044034No Hit
GCAATGGGGGGATTATATTTGAGAGTAAATGAAGTGTGTATCAGTAATGTCA1200.12285892723680034No Hit
AATTGTGGGGATCAAAGCCAATCTTTGAACATCCACATTGCTGAGCCTAACT1190.12183510284316033No Hit
GCCCCTGGGGAATACAGGCAGAAGGATACAGCTTGATGCTAACACTGCATGA1180.12081127844952033No Hit
TGTTGTGGGATTATCACAGGGGAAACATAGAATTTTGCTTACCAAACGTTAA1180.12081127844952033No Hit
CGCAAGGGGCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGC1180.12081127844952033No Hit
ACGTCCCGGGTGGACAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGTC1180.12081127844952033No Hit
AGGCACGGGAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAATGCAG1160.11876362966224033No Hit
GTCACACTGGGGAAAAAATTAAGCATATATTAAGTTTCTCCAGCATGAACTA1150.11773980526860033No Hit
GGATGCGGGAAGAACAGTAATGATAACCTAAGGCAGTTTAACAGTATGGAAT1150.11773980526860033No Hit
TTATGCGGGGAAAAGAGAGATTTTTGGAAAGCACCCAAATTTAAGAGAGTGC1140.11671598087496032No Hit
CGACTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1140.11671598087496032No Hit
ATTTCAGGGTGGCAACCTTGATGATGATGTATGTGAAGATTGGAGATAAAAG1140.11671598087496032No Hit
GGAAATGGGGTATTACTGCTGGGATTTGTTTTATTATTCAGACTTTAAGATC1130.11569215648132031No Hit
GATTACGGGATGTTGAGGCAGGAAGTTTAGTAGAATTCCAAGGAAGTAAGAG1130.11569215648132031No Hit
GCCAATGGGGGAAATAATTTACCAAAATAACTGAGTGTTGCTGCTAAAGAAC1120.1146683320876803No Hit
AATGCAGGGCCATAGTACTTTTTCATGAAAGAAATTTGCTAAATAAGACATG1120.1146683320876803No Hit
TACAGGGGGATTGCATGCAAGTTTGCTGAGCTGAAGGAAAAGATTGATCGCC1100.1126206833004003No Hit
ATCATGGGGGGCCCCTGCCACTGGGCCATGGAGACTGTGGCACAGTAGACTG1100.1126206833004003No Hit
TATATAGGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTG1100.1126206833004003No Hit
TGAGTTGGGAAGCAAAAATTAGAATTTTGGAAAACTCATTTGCCACCGTGAT1090.1115968589067603No Hit
GTTCAAGGGGGAAAAAACAGAGTGATGTTTTACTCAAATGCTTTCTAAGTAC1090.1115968589067603No Hit
GACACTGGGGGCACATTGTTTTTTTGTGTTGTTCCTGTTTTACTAGCTTTTA1080.1105730345131203No Hit
ACCTAGGGGACATCATTGAGATTTAAGGCAAAAAAGTGGCTCTACCCAGTCT1080.1105730345131203No Hit
GCATGAAGGGAGGCCATGTGAGCCACCGCTGCCAGCACAGAGCGTCCTTCCC1070.1095492101194803No Hit
AACTTAGGGAATGCATTCTGCACAGACTCAATTTCACGAACTCTGTTGCGGA1070.1095492101194803No Hit
CCTAGCGGGATGGGCATAGAGGTAATTCTCTATTTCACTTTGGTGGAAGGAC1070.1095492101194803No Hit
CTAATCAGGGATCCAGAATCTTTAAGGAACTTAAGCAACTGAACAAGCAAAA1060.10852538572584029No Hit
ATCTTAGGGCCCAGGCTCAGGGACTCTGGTGCCAAATCCAGAAAGATCTGCT1060.10852538572584029No Hit
CATCTTGGGGGAGAAGGGTTTTTTTCAGGCTGCGGGTCGGAGGGCAGAAGTG1060.10852538572584029No Hit
AGTATTGGGAGATTTCTAGATACTTTTTGCTGCTAGTTTTATGTAATATTTA1050.10750156133220029No Hit
TCGGTTGGGGGGACTTCTAGTACAGCCCAGAATGGATTTGGCATTATTTATG1050.10750156133220029No Hit
GGGTTGGGGATTTGACTTTGACATTTTGTGGTTAAAATTATTCTGTACTCAT1050.10750156133220029No Hit
GTCACTGGGCGATCTAAAGAAGGGCATGCCTCCCTGATAGACTTCAAATTCC1040.10647773693856029No Hit
CGCAGGGGGACCTTAGTGGAAGATGCTCATCTTTTATTTATTTATTTTTCTA1040.10647773693856029No Hit
TATGCAGGGCGATCTCGTTGGGGCATCCATATTTGTTTCTGGAATTAAAACT1030.10545391254492029No Hit
TGATGTGGGGGAAAACTCCCGCTTATAAAACCATCAGATCTCATGAGACTTA1020.10443008815128028No Hit
GCTGAGGGGGAAAAAGTCACATGCATTTCTATACACCAATAGATAAGCAGAG1020.10443008815128028No Hit
ATAGCTGGGACCAAAAATGATAGTCACTGAAGGAAACCAAATCATAATTTTG1010.1034062637576403No Hit
TTACTAGGGCTGAGAACACGGTGTGTCTCGACACGTACCACGTACGTGGAAA1010.1034062637576403No Hit
TATAATGGGGCTGTTCACATTAAGCAGTTTATTAGGGGGTTATTTTGAAATC1000.1023824393640003No Hit
AAAATAGGGGTATATTGATATATACAAATATATATCAGTAGAATATTGACAA990.10135861497036029No Hit
TATGCAGGGAAGATTATTCAAAACGTATTTTAAAATAATTACCACCATTGAT990.10135861497036029No Hit
GTTACCGGGAATGACTGGGAAACAACTATAGAAAACTTTCATGTGGTGGAAA990.10135861497036029No Hit
CAGTTCGGGAACACATCCAATAGAAACATTGGCAGCATGGTCTTCCAAAACA990.10135861497036029No Hit
ATGCGCGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT990.10135861497036029No Hit
AAGATTGGGACTGGAGCTGTGTGTCCCATTATCTGCTGGTCATGCCTGCCTA990.10135861497036029No Hit
TTAATAGGGGTGCTGCAGGCTTGGAATCTTAATGAGGCCTGAGCCCTGACCG980.10033479057672029No Hit
TGCTTGGGGATTCCTGTTTTTATCCAGCAAGATGTAAGATGATTTAATATTT980.10033479057672029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)