FastQCFastQC Report
Sat 31 Dec 2022
SRR5846317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184106
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT10650.5784710981717054RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC8650.46983802809251196No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC2900.1575179516148306No Hit
GCGCTTGGGTAGAGAAAAGTTGGCTGACAAGAATGTTCTTTATCCAAATTTT2710.14719780995730722No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA2300.12492803059107253No Hit
GCTCCTGGGCAAACACCTGGCCATTATTAGCTGAGCAAGACAGCAGTGTTTT2050.11134889683117334No Hit
TACGCCGGGTTAGTGTTCTGTCCTGTTCACCATCTGCTACTGCTTCCCACAC2050.11134889683117334No Hit
GTCTGTGGGTACCACTCCTATTCAACATAGTACTGGAAGTCCTAGCCAGAGC1890.10265825122483788No Hit
GAGAAAGGGAAGACATAGAATGGCTGAATGCATAAAAAGCAAGACTCATTTA1880.1021150858744419No Hit
CTGTTGCGGGATTATTTTAAAATGTTTTCTTAAAATGTTTTACCATATATTT1880.1021150858744419No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)