FastQCFastQC Report
Sat 31 Dec 2022
SRR5846316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184049
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT9740.5292068959896549RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC8110.44064352427886055No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC2860.15539340067047364No Hit
GCGCTTGGGTAGAGAAAAGTTGGCTGACAAGAATGTTCTTTATCCAAATTTT2520.13692005933202572No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1980.1075800466180202No Hit
CTGTTGCGGGATTATTTTAAAATGTTTTCTTAAAATGTTTTACCATATATTT1930.1048633787741308No Hit
GTCTGTGGGTACCACTCCTATTCAACATAGTACTGGAAGTCCTAGCCAGAGC1920.10432004520535292No Hit
GTCTAAGGGAACCCAGAATCAAAAGACCACAGATTATGTGATTTCCTTCACC1860.10106004379268564No Hit
GAGAAAGGGAAGACATAGAATGGCTGAATGCATAAAAAGCAAGACTCATTTA1850.10051671022390776No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)