FastQCFastQC Report
Sat 31 Dec 2022
SRR5846308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203359
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC7900.38847555308592197No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT4960.24390363839318646RNA PCR Primer, Index 24 (95% over 21bp)
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2930.14408017348629762No Hit
GGTCATGGGAATGATATTGGACAAAAAATTTACAGCAAAATTAAGTCAGTTC2310.11359221868714933No Hit
GCCTACGGGAAGCATTCATGCGGGAGAGAAGGTAGAGATTGAAGGAGCTCAT2230.10965828903564633No Hit
CGAATCGGGAAGATAATGCAATGTAAGTGTTTGCTACCAAAAATTATTATTC2100.10326565335195394No Hit
TAGGAGGGCAAGAGTGAGATGTAGAAAGTTGTAAAATAGAAAAAGTGGAGTT2070.10179042973264031No Hit
ATTCTGGGGCTTGCTGGGGACAAATGAATCAGTTATGATTTGGCAACAGATG2060.10129868852620243No Hit
CAAAATGGGGACAAAAAATCATGAAATAAATTCATTTTAGGTCAGTGTCCAT2050.10080694731976454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)