FastQCFastQC Report
Sat 31 Dec 2022
SRR5846306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202166
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC7430.3675197609884946No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT5130.2537518672773859RNA PCR Primer, Index 24 (95% over 21bp)
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG3000.14839290484057657No Hit
GGTCATGGGAATGATATTGGACAAAAAATTTACAGCAAAATTAAGTCAGTTC2380.11772503784019074No Hit
AAGCATGGGAAGTAGAAAAGAAACTAAAAATGGATTTAGTGTGAGGACAGGT2070.10239110433999782No Hit
AGCTGTGGGGCTTCAGAAGGCAATAGAAGAGAACAACAACTTCAGTAAAATG2050.10140181830772732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)