FastQCFastQC Report
Sat 31 Dec 2022
SRR5846305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168659
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC8710.5164266359933356No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT8700.5158337236672813RNA PCR Primer, Index 24 (95% over 21bp)
CAAGGTGGGAGCCAAAGACCTGCCGTGGAAGAGCTGGAGAGAAGAAATATCT1870.11087460497216277No Hit
ATAAAAGGGGCTCTTCCGATCTTGTGGTGGGGAAAAATCGAAGAGCAACAAA1860.11028169264610842No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1850.10968878032005408No Hit
TATTGCGGGACAGGAGGCTTTTAACTTTGTGAAAATCCCATGTTTGCTTTAT1730.10257383240740191No Hit
GATTGAGGGGCACCGTGGAACTTGTATTCGACAAGCATTTGGAAAACTGTTG1700.10079509542923887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)