FastQCFastQC Report
Sat 31 Dec 2022
SRR5846304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169209
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC8940.5283406910979912No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT8820.5212488697409712RNA PCR Primer, Index 24 (95% over 21bp)
CCAGATGGGGATCTGTTTTCGGGTAGCTCTTTGTCTTCCTGTGTGACCTTGT2090.12351588863476529No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1940.11465111193849026No Hit
CAAGGTGGGAGCCAAAGACCTGCCGTGGAAGAGCTGGAGAGAAGAAATATCT1860.10992323103381026No Hit
ATAAAAGGGGCTCTTCCGATCTTGTGGTGGGGAAAAATCGAAGAGCAACAAA1850.10933224592072525No Hit
CTACGCGGGGACTGGGAAGGAAGCCTGTGATGAGGCTGAGATAAAGCACAGG1790.10578633524221524No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1780.10519535012913026No Hit
GTCGTAGGGTCATGGCCAGAAGAGAAAATGTCATTGGTTTGTGCCCAAGCAA1730.10224042456370523No Hit
TAAAAGGGGATGGCGACGGTGGGGGCTCCGCGGCACTTCTGCCGCTGCGCCT1720.10164943945062024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)