FastQCFastQC Report
Sat 31 Dec 2022
SRR5846303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168676
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT8120.48139628637150517RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC7880.4671678247053523No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1950.11560625103749199No Hit
GAAAAGGGGCAGTCCCAGGAGCCAGATTTTTTCCTCCATGAGAGAAAATAAA1760.10434205221845431No Hit
CAAGGTGGGAGCCAAAGACCTGCCGTGGAAGAGCTGGAGAGAAGAAATATCT1730.10256349451018522No Hit
TTATGCGGGCTCAGAGGAGTTTTGGAGGTGGTAATTGGTGTGGCAACTGGAT1710.10137778937133912No Hit
GTCGTAGGGTCATGGCCAGAAGAGAAAATGTCATTGGTTTGTGCCCAAGCAA1710.10137778937133912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)