FastQCFastQC Report
Sat 31 Dec 2022
SRR5846302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120438
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC5060.4201331805576313No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT4760.39522409870638836RNA PCR Primer, Index 24 (95% over 21bp)
AAGTATGGGGGCTCAACTCAAGGAAATTTTTCTTTGGATAAAAGAGGTTTTT1590.1320181338115877No Hit
GTTAATGGGCCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.12454540925621481No Hit
ATGACTGGGGGGACTGGCCGTGGAGGAGAGTCTATCTTTGGCCAACTGTATG1440.11956359288596621No Hit
TACCAGGGAGCACATTAGCAAACAACAAACACTATTGCACGGTGCAGAGTTT1370.11375147378734286No Hit
TTTTATGGGCAGCTACATGTGCTCCCAGGTTACGGGTTAATAATGGATACAA1360.11292117105896811No Hit
GGACCAGGGGTAATGATTGTCACTTATGTCTAATATCACAAAATATTCAAGA1350.11209086833059334No Hit
GATCAGGGATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCGAATAAAC1350.11209086833059334No Hit
CAAGAAGGGTTTCCATGCCATGATAAACTCTTCCGTGCATGTCATAATGTAC1340.11126056560221856No Hit
CAAATGGGGGCAAGAACCTAACTTTTACTCAGGAGCTGAGAAGGAAGAAGCC1340.11126056560221856No Hit
ATCCTCGGGGTATAAAGCAAATTATCACAAAATCAGATGGGCAGTTACATGT1320.10959996014546904No Hit
GCAACTGGGATCTTGACGCGGGCTACCCAGTTCTTATGTAACACTAAGGATA1320.10959996014546904No Hit
TTGTAGGGGGCATGAACTCAAGCAGTGGTACAAAGGATTTCTCAAGGACTGT1310.10876965741709428No Hit
AGAACTGGGGTGAAATGCAAATACACAGTCACAAATCATCAGACTTTTTAGG1310.10876965741709428No Hit
GTGCACGGGAAGCATTTTGATCCATTTACTGAAGGCATCGTAGATGGGATAG1300.10793935468871951No Hit
CAAGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.10710905196034473No Hit
CTAAATGGGGGGCGGGAGAAGCGGTGGTAGCGGAGGCTGTAGTGGGGCTGGT1270.10544844650359521No Hit
TGTAATGGGATGTATGGAAAGGTCTTAGCATGGATTAGAGCAAACAAACTTA1270.10544844650359521No Hit
TAGCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1260.10461814377522044No Hit
CAAGTGGGGATCTTGCCTCGGGATATGTGGAAAGGCCTTCATTCATAGTTCG1260.10461814377522044No Hit
GCTGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1250.10378784104684569No Hit
TCAAAGGGGCTACCTTTAGATCTTTAGATGATTTTATAGAAGGATGGGTTTT1250.10378784104684569No Hit
GAAACTGGATCCCTTCCTTACGCCTTATACAAAAATCAATTCAAGATGGATT1240.10295753831847093No Hit
GCCAAGGGGCCGATCTTGACAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGC1230.10212723559009614No Hit
AGCTTAGGGGCCTCTCAAAGTGCTGGAATTACAGGCATGAGCCACCTCGCCT1220.10129693286172138No Hit
AGGGCCGGGCAAGAATGAAAACAGAAAATCCAATGTTGTTATGAAAATAGAA1220.10129693286172138No Hit
ACATATGGGATAGGACACAGGTAGCATCCCTCTAGTCATTGGCAATGGCTCT1210.10046663013334661No Hit
TGGTCAGGGATTGCTGAACAGCAAATGTTGCTGACTGACCGTTCCTCTGGAA1210.10046663013334661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)