FastQCFastQC Report
Sat 31 Dec 2022
SRR5846301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121616
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC4860.39961847125378236No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT4620.3798842257597685RNA PCR Primer, Index 24 (95% over 21bp)
TTTTATGGGCAGCTACATGTGCTCCCAGGTTACGGGTTAATAATGGATACAA1580.12991711616892515No Hit
GTTAATGGGCCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.12498355479542167No Hit
AAGTATGGGGGCTCAACTCAAGGAAATTTTTCTTTGGATAAAAGAGGTTTTT1470.12087225365083543No Hit
AAGCAAGGGGGAATTAAGTTGATCTGCCCTTATTATAACTTCAGAAAAACAT1460.12004999342191816No Hit
CAAGTGGGGATCTTGCCTCGGGATATGTGGAAAGGCCTTCATTCATAGTTCG1450.11922773319300091No Hit
AGAACTGGGGTGAAATGCAAATACACAGTCACAAATCATCAGACTTTTTAGG1410.11593869227733192No Hit
TATGAAGGGGGAGTGCAGTGACGCGATCTCCACTCACTGCAGGCTCCGCCTC1400.1151164320484147No Hit
TACCAGGGAGCACATTAGCAAACAACAAACACTATTGCACGGTGCAGAGTTT1390.11429417181949744No Hit
TATAAAGGGATACTTACCCGAGGTGTCTGCCTTTGTGCTTAATTGAATTGTC1390.11429417181949744No Hit
CAAGAAGGGTTTCCATGCCATGATAAACTCTTCCGTGCATGTCATAATGTAC1380.11347191159058019No Hit
AGCGTAGGGCAGTATATCTCAGATCTCATGATGAAGTTTCCTAATCTAGAGG1380.11347191159058019No Hit
GGATACGGGAGAGGGCGGAAGCGCTATCCGAGCAGGATGCGGTTCGTGGTTG1360.1118273911327457No Hit
AAGAAAGGGTATGTCCTACATTTGAGTATTAGCATGAGAATAGGAGAATTAC1340.1101828706749112No Hit
AATACAGGGGTATGAATACAACGGGAAGAGTGGCTACAGATTGAAGCCAGAG1320.10853835021707671No Hit
ATGACTGGGGGGACTGGCCGTGGAGGAGAGTCTATCTTTGGCCAACTGTATG1310.10771608998815946No Hit
GGACCAGGGGTAATGATTGTCACTTATGTCTAATATCACAAAATATTCAAGA1300.1068938297592422No Hit
AGTTTTGGGTTAACATAACCTCTACATGTATATAAGATAGCTGGTGAAAATG1290.10607156953032496No Hit
AGTTCAGGGACAGATTCAACGCTTTTCCTACCAAACTACCAATGTTATTTTT1270.10442704907249047No Hit
GCAACTGGGATCTTGACGCGGGCTACCCAGTTCTTATGTAACACTAAGGATA1270.10442704907249047No Hit
GTACAAGGGGTGGTCCTGGGAGTCACAAACCCTTTCTTTATCAAAACACTCC1270.10442704907249047No Hit
GAATATGGGATACGAACTTTAGTGTCAATTAGTCTAACTTCATAAAATATCA1250.10278252861465596No Hit
AATCAAGGGGAACTTTTGGGGTGGAGACTAGAGGCAGGAGCTGAGGGACTGA1250.10278252861465596No Hit
AGCTTAGGGGCCTCTCAAAGTGCTGGAATTACAGGCATGAGCCACCTCGCCT1230.10113800815682147No Hit
TTGCAGGGCTTGCAGGCTGTACCTCTGCCCTTCCTTTTCTCATCAATCACTG1220.10031574792790422No Hit
GATGGAGGGCAAGGCTATAGTAAGCAAAACGACATGGTATTGGTATAAAGGT1220.10031574792790422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)