FastQCFastQC Report
Sat 31 Dec 2022
SRR5846300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121615
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC4740.3897545533034576No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT4540.373309213501624RNA PCR Primer, Index 24 (95% over 21bp)
AGAACTGGGGTGAAATGCAAATACACAGTCACAAATCATCAGACTTTTTAGG1780.14636352423631954No Hit
AAGTATGGGGGCTCAACTCAAGGAAATTTTTCTTTGGATAAAAGAGGTTTTT1570.1290959174443942No Hit
GTTAATGGGCCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.12662911647411915No Hit
TATGAAGGGGGAGTGCAGTGACGCGATCTCCACTCACTGCAGGCTCCGCCTC1470.12087324754347736No Hit
GGACTGGGGTAACATCCAAATAACAGAAGTCCCAAAAGGAGGGGAAAAGTGA1420.11676191259301896No Hit
AAGCAAGGGGGAATTAAGTTGATCTGCCCTTATTATAACTTCAGAAAAACAT1390.1142951116227439No Hit
GCCAGTGATGATGTTTTGATATTTATTTCCTGCTACTTAAATTTGAATCTGA1350.11100604366237718No Hit
ACTCATGGGTAAAAGCACACTCTGGCCAACTGTTTTGTCATATAAATATAAC1340.11018377667228549No Hit
TTGTAGGGGGCATGAACTCAAGCAGTGGTACAAAGGATTTCTCAAGGACTGT1330.10936150968219381No Hit
CTACTTGGGGCCTGCTGAGTGAGGCCAGTTCCTCATCAGGCTCAGGGCAGGT1330.10936150968219381No Hit
GTTAGGGGGAGAAATTAACAAACAAAAGACAGCCCCACACGGACCAGTGTGC1330.10936150968219381No Hit
CAAGAAGGGTTTCCATGCCATGATAAACTCTTCCGTGCATGTCATAATGTAC1310.10771697570201044No Hit
AGGGCAGGGTAAGCACTTAATAAAAACTGCAGCCAGAGTACCAGCCAGTGTG1290.10607244172182709No Hit
GATCAGGGATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCGAATAAAC1290.10607244172182709No Hit
GAAACTGGATCCCTTCCTTACGCCTTATACAAAAATCAATTCAAGATGGATT1280.1052501747317354No Hit
TTTTATGGGCAGCTACATGTGCTCCCAGGTTACGGGTTAATAATGGATACAA1270.10442790774164372No Hit
TCAAAGGGGCTACCTTTAGATCTTTAGATGATTTTATAGAAGGATGGGTTTT1260.10360564075155203No Hit
TATAAAGGGATACTTACCCGAGGTGTCTGCCTTTGTGCTTAATTGAATTGTC1260.10360564075155203No Hit
TTCCGCGGGTGTGAGCAATTGTATCAATTCTTGTTCTCTGTTGGAAAGAGCA1250.10278337376146034No Hit
ACCGGCGGGATGGATAGCTTGCAGAAGCAATCACAGTTTCTTTCTAAAGAAA1250.10278337376146034No Hit
TACCAGGGAGCACATTAGCAAACAACAAACACTATTGCACGGTGCAGAGTTT1250.10278337376146034No Hit
AGTTTTGGGTTAACATAACCTCTACATGTATATAAGATAGCTGGTGAAAATG1250.10278337376146034No Hit
CAATGCGGGGACATTTAAAAAGAATTAAGTACCTGTCAAATCTAGCATTGAG1240.10196110677136866No Hit
GGGAAAGGGAGAAGAACGAGATGAGGTCCTCCACTGCCAATTTTCTGATAAC1220.1003165727911853No Hit
GGCCAGGGGTAGACTTTGTGATCATAGTATGTCTGAAAAATGAAACAGTCTG1220.1003165727911853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)