FastQCFastQC Report
Sat 31 Dec 2022
SRR5846293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175239
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC6930.39545991474500536No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT6390.3646448564531868RNA PCR Primer, Index 24 (95% over 21bp)
TCTCCTGGGACCTTCAAGAAATATGAGATTATGTAAAGCAACTAAACCTATG2070.11812439011863797No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC2020.11527114398050661No Hit
ACTCATGGGTCCTCAGGGATTTCATTTTGCAGGCATGTCTCTGAGCTTCTAT1990.11355919629762781No Hit
ACTAATGGGGCTTCCCGAGGGCGGCACGAGGGCTGGGCGGTGGGGTGCGGGT1920.10956465170424393No Hit
GGACGTGGGATTCTGTGCTGTTATAAACAAACCCAATATGATGCATTTTAAG1910.10899400247661765No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1840.10499945788323375No Hit
TGGGGAGGGATGGTTTTTCCTTTTCTTAGCATGAAACCTCTCACTTCCTCAC1820.10385815942798123No Hit
CAAGATGGGCCCCCCAAAAAATGACCCAGCTTAGAAAGAAATCATAGATGAC1780.10157556251747613No Hit
ATCCGTGGGGGAAGACAAAAATGGAATGTTGTAAGTTTTTAAATGTCCTGAA1780.10157556251747613No Hit
GTCGGGGTTATAGATTGCCTTACACATCTTAAAAACAAATACTTTGGGGGGT1770.10100491328984987No Hit
TCACGTTGGGAAGAAAGACTAAGAACGAATGAAAGAGAGACAGAACCCAGAA1770.10100491328984987No Hit
TTACATGGGGACGCAGAGGCGGACAAGATGGCGGCGGCAGCTGTACAGGGCG1760.10043426406222358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)