FastQCFastQC Report
Sat 31 Dec 2022
SRR5846292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173989
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT6530.3753110828845502RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC6370.3661150992304111No Hit
ACTCATGGGTCCTCAGGGATTTCATTTTGCAGGCATGTCTCTGAGCTTCTAT2170.12472052830926093No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC2130.12242153239572617No Hit
TCTCCTGGGACCTTCAAGAAATATGAGATTATGTAAAGCAACTAAACCTATG1920.11035180384966865No Hit
ACTAATGGGGCTTCCCGAGGGCGGCACGAGGGCTGGGCGGTGGGGTGCGGGT1890.10862755691451759No Hit
GGGCAGGGAAAATGGAGAAAATATAAAAAGATGTGAAAAAGGAAAAAGGAAA1890.10862755691451759No Hit
TTTCGCGGGAGCTATTCGAGGGAGTGAAATACAGCTGGTTTGCCTTCGAGAA1830.10517906304421544No Hit
TGTTAAGGGGACTTTAAAAATTTCCAAAAGGTCAGGAATTTTTGGAGACTTT1820.10460431406583175No Hit
ATTATGGGGTGTGTTAAAATCCAGAAAACTATACACCAATTAAAAAAACTCA1800.10345481610906436No Hit
TTGATTGGGAACATCATGGGCTGTTAAGGAAATGCAAATCCAAATCATAAAG1780.102305318152297No Hit
TTTGTGGGGCATATGTGAAATTGTATGGTTCTATTTATGGCAAACAGAAGGA1780.102305318152297No Hit
ACACCCGGCCTGGAAGATTTTTTTTTCTTCATTCTGTTTTCCTATTTTTCAA1770.10173056917391331No Hit
TGTATGGGGAGCCACAAGATTACTCTCCACTTGCTTAATAATATATCTTAAC1750.10058107121714592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)