FastQCFastQC Report
Sat 31 Dec 2022
SRR5846291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171454
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT6080.35461406558027225RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC5910.344698869667666No Hit
CGCAAGGGGGGCATTCTGTCAAAGCACTGGTAGATTGTACCTAACAAGTTTT1900.11081689549383507No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1810.10556767412833762No Hit
AGCTTAGGGCACAAGATGCTCGACAAATACATGTTGAAAAAACAAGTACATA1790.10440118049156043No Hit
AATTATGGGATAAGTCACCTTGGTAAACATAACATTTGGCTACCTTCTCATG1770.10323468685478321No Hit
CACGTAGGGGAGGCGGAGCCGCCGAGCTCGCTGTGGCCCGGATGTTCGGTGC1760.1026514400363946No Hit
ACACCCGGCCTGGAAGATTTTTTTTTCTTCATTCTGTTTTCCTATTTTTCAA1760.1026514400363946No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1740.10148494639961739No Hit
GGGCAGGGAAAATGGAGAAAATATAAAAAGATGTGAAAAAGGAAAAAGGAAA1740.10148494639961739No Hit
ACTCATGGGTCCTCAGGGATTTCATTTTGCAGGCATGTCTCTGAGCTTCTAT1740.10148494639961739No Hit
TTTGTGGGGCATATGTGAAATTGTATGGTTCTATTTATGGCAAACAGAAGGA1730.10090169958122878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)