FastQCFastQC Report
Sat 31 Dec 2022
SRR5846290.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846290.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69722
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT5660.8117954160810074RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC5070.7271736324259201No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1440.20653452281919624No Hit
AAATCGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1290.18502051002552997No Hit
AGGCATGGGACATGTGTTTTTCTGGGGAAAATGGGTCCATGATACTCTAAGG1180.1692435673101747No Hit
GCTGCGGGGAAAGGCAACAGGCTGTTATGCAGAGCCTGTTGCCTTTCCTCGC1150.16494076475144143No Hit
AGAGGTGGGTTGGATGTTTCCTACAAAACTAAACACTTTTACCATAAGATCC1140.1635064972318637No Hit
GCGTTTGGGCTGGCTTGCCTTCTCATTTTCTTAATAGTGTCCCTTGGAATAT1120.16063796219270818No Hit
TTAATGGGGATTCTGTGACGGAAAGCCTATGTTGACCTCGTCCGGCACTCAA1080.15490089211439717No Hit
GAACTGGGGGATCTAAAGCAGGGATTGGATGGATATGGGAGCTGTGGCCCTC1070.1534666245948194No Hit
TTGCTGGGGCGATCTTACTTGGGGGTTGTTTTTTTGAAAAGATCAACAAAAT1060.15203235707524168No Hit
GGCACTGGGTAGACATCTTCATGCCCGTTAGTTCATCGTTTGCCTAGCATGT1050.15059808955566392No Hit
GTTTTAGGGCTTCTTTCCCATCTGGCGGCCGCGGCTCCTGTCCAGACCCTGA1050.15059808955566392No Hit
ATGCAGGGGACCCATTTGAGTGATATTGCACAACAAACACAGATACCTACAG1040.14916382203608616No Hit
GCAATTGGGGATCTTAATTGGGGTGATCTTTGTAGATTGGTTATTTCTGTAC1040.14916382203608616No Hit
GCGCGAAGGGAGTCAAAAGAACAGGAATTCGGAAAACTCTTCAGTAAATATC1040.14916382203608616No Hit
TAACCAGGGCATCAAGTGCATAGCTTTTAGCTTTTAACTGAATTTTTCTGGG1030.14772955451650843No Hit
TAGTCTGGGAGTTTTAAGGAGATTCATAAACAGGTGTAATAAGATGTGATTA1010.14486101947735291No Hit
CAAAAAGGGAGGTGGTTCTCCGTGTGTTTATATACACAGACGATCCCCGAAT990.14199248443819742No Hit
ACGCTAGGGGGCTCAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTC980.14055821691861967No Hit
CTAACTGGGAGGCACTCACCAAATTCTTATTGAATGAATGAGTGAGTCAATG970.1391239493990419No Hit
TCAAAAGGGGTTTGAAAATTATGTTCAGGGACTAGTAGGAGAGGGGAGCTAT970.1391239493990419No Hit
GCATGGGCACAAATATATGGTATACATAGCATATAGAAAGAAAACTAGACCA970.1391239493990419No Hit
TTAAAGGGGGCCTCAAGCCCTGCCTCTTGTCACAGTGAGGGTTCTGAGAATA970.1391239493990419No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA970.1391239493990419No Hit
ACGCAGGGGTTTATAAGAATTCTAACCATTGATATTCTTACATTCTTGCCTA960.13768968187946415No Hit
GAGAAAGGGCTGGTGATTCAAGTGAATTTTAAAGCATGTTTATGCTGTTGCA960.13768968187946415No Hit
TGCACTGGGAAGCAAGAGGAAAAAAACAACACAATCAAGGGACAAACTACAT950.13625541435988642No Hit
AGATTAGGGCACGGTAAACTTCATTCATAGATCCTCCCTTATTTAACATCAG950.13625541435988642No Hit
TATGTTGGGCAAAATTGGAGAAATTACCATTAAGGGTCTCTTCCTGACAATT950.13625541435988642No Hit
CAAATGGGGACAAGAATGTTGTCTTCTAACTCTGACATTATACCTTGTTTGT950.13625541435988642No Hit
ATCGTCGGGTTGCTAGACCCAGCACCACTTATAAATTTAGTACACTGTTCAG950.13625541435988642No Hit
TTGTGCGGGAAGGGGACTGCTTTGAAGGGCCATTGTTCATTTGGAATGATAA940.13482114684030866No Hit
AGACTTGGGAGTAGAAACTGTGAACTCTGTGACTTTGACTCAGGACACAGAA940.13482114684030866No Hit
ACGTACGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC940.13482114684030866No Hit
AAGCCAAGGGTGGAGTGACATGCCCCAGATTATAAAATAGGTGGGCAGTGTG930.1333868793207309No Hit
GTTTCAGGGGCTCACTGTAGCCTCAATTTCCTAGGCTCAAGGGATCCTCCTG920.13195261180115314No Hit
CAAATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA910.1305183442815754No Hit
TCACTAGGGATGTCAGTTTGCAGTTATGCAGAATCCAAAGTAAATGTCCTGC910.1305183442815754No Hit
TACAGAGGGGGACTCTCTCTGTAAGGAGGGAAGGGGGAGAAGGTAGAATTCT910.1305183442815754No Hit
AGACTAGGGATAACAGGAGTGCCACTTCATGGTGCGAAGTGAACACTGTAGT910.1305183442815754No Hit
ATAAATGGGTACAAAAGAAAACCTGTAGAAGTTTTTACAAATATAAGAAATT900.12908407676199765No Hit
CGTTTGGGGATTTTAACAAAGTAATATTTTTGTATTGCATTTTTCTATTAAA900.12908407676199765No Hit
AAGATAGGGATGGGTTCAGTGGAAATGTTTGGAACTCTGAAGGATTTAGACA890.1276498092424199No Hit
GACAGTGGGGTTAATTCATGAACAGGATATAACAAATGTAAATATATATGCA890.1276498092424199No Hit
AGCTCTGGGGATTTCCTGGTTTCCTCCTGCATTCTGCTTTACATTCTTGAAG880.12621554172284213No Hit
GATTGGGATAGTGCCAACACGTGGCGCGCCTTTGATGGCCCGGGTGCACACC880.12621554172284213No Hit
GACAAAGGGGAGAGAAGCCGCCACTCGCAACATGGAGAAGATCCAGGTCATC880.12621554172284213No Hit
TTGTCTGGGAGGAAGCGGAAGCGGAGAGGCGGTCGGGATCCGCTGCGCGAGC870.12478127420326439No Hit
ATTATTGGGGAGGCTCGCTGCGCTCCCTGTTGCCCAGCGCGGGCCCGTTGAG870.12478127420326439No Hit
TTGCAGGGATTTAAGGATGGTAAATTTGGGCATTTTAGATGTCTTTATTTTA870.12478127420326439No Hit
CGGCCGGAGGGTGTGTCTTTGTACTTGGTTCATTGCCCTTCACCTGGTAGAG870.12478127420326439No Hit
GTCAACGGGGTAACCTTGGTGTGATGTAATAATGACATTATCTGCTGGTCTT860.12334700668368666No Hit
TAGTCAGGGCTGGATTAGATGCATGAATTTTGAAAATACAACTTACTAGCAC860.12334700668368666No Hit
TAAGCAGGGATACCCTGCTGGATTCATGGATGTCATCAGCATCGAGAAGACA850.12191273916410889No Hit
ACTTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC850.12191273916410889No Hit
TCTAATGGGGAACACACATTGGAGAGAAACACTATGAATGTAAGGAATGCGG840.12047847164453115No Hit
CGAATGGGGAACCCTCTGTAGCTCACACCGTTTTCTGAACTATAAAATGGAG840.12047847164453115No Hit
CTCGCTTCGGGAAGTGCTGGGATTACAGGTGTGATCCACTGCACCCGGCCGG840.12047847164453115No Hit
GGCTCTGGGCGCTGCCATAGTTTTTTACCACATTCCTAATGGTTTTCAACCT840.12047847164453115No Hit
GTCATGGGGTGGTTAGTGAGAAGTAAAATAATGGAAAGTAAAATAATAAAAG830.11904420412495338No Hit
CACATTGGGATTGGTCCTTCCATTATTTCTTTAGGAAAGTTGTACTTTTAGA830.11904420412495338No Hit
GTTGTTGGGGTCACACTGGCCTTGCCCTTTCCTATCCTTAAGCTATGCCACC820.11760993660537564No Hit
GTTCCTGGGATTTTATTAATGAGTGGAGTGAGGTGTGGTAGTAGAAAGATCA820.11760993660537564No Hit
TAAGCTGGGTGTTGAGTGAATTAATGAATTAAGAGTGGCTGGCAGAGTAGGC820.11760993660537564No Hit
TAAACATGGGTGAGGGATTTCCTGGGACATGAGACTTTCAGAGATAATACCG820.11760993660537564No Hit
TAAGTGGGGCCGTTAATTTGTTGAATTAAATGCCTTTATAAAAATATTTACA820.11760993660537564No Hit
GGTAGTGGGCCAATGGGAAAGGCAACAAGTAACATAAAGGCATTTGTCTTCA820.11760993660537564No Hit
CCGAGAAGGGGATGGAGAGGGATTCCTAACCCATCTGGATATGACATCTGGG820.11760993660537564No Hit
TGTCTGGGGTTAGAAACTTACTGGAAATGCTGACACGGATTTTATGCCTTTT820.11760993660537564No Hit
TCAGTTGGGTGGCATAGCTTTAGTAATGTATGGGAGGATGGAAGAGGCTGAT810.11617566908579788No Hit
CTGCCGGGGAAAGTGAATGGATGACTGTTCATGAGAAAAAGCAGGGTAAAAA810.11617566908579788No Hit
AGACCTGGGATCTCAAAATGAGATTTGGGCAGGGACAAATATCCAAACTATA810.11617566908579788No Hit
AATGCTGGGGAGTCAGTCTGACGGTCAGTGGATCGGTGGGTTTATCTCAAGG810.11617566908579788No Hit
GGGTTCCGGGTTATTTGAAAATCCAGTCCTTTATTAATAGAATGGAGGAAAG810.11617566908579788No Hit
CTTTTGGGGGCTCTGAGAGATTCCTCTGCGAATCTGTTTTTAGCTATCAAGT810.11617566908579788No Hit
ACCTTGGGACAACATGTTGGTAGATAAACTTAATACCAACACTGAAATGGAA810.11617566908579788No Hit
TGTTATGGGATGGTCTCTGGGATTTACTCAACAAGTCTCACTTTCCCCATGT810.11617566908579788No Hit
ATTTATGGGTGTTCTGGCAATTCTTGTTTCTTGATTTTTCTATATAAATTTT800.11474140156622013No Hit
GTTACTGGGACCGGGAAACCACTGATCAATCAAGGAATTGACGTGGTCCGTG800.11474140156622013No Hit
AGCCATGGGCTGTGTCTGGGCAATGACAGTCGCATAAGGATTAGGCTCACTC800.11474140156622013No Hit
CTCGCCGGGAGATGAGACATGTTGTAACCCTGTGGCCTGTTTTCTTATTTTG790.11330713404664237No Hit
GGTAAAGGGGGATATGACACGCGATATCGCAGTAGAGATGGGGTTTCACCAT790.11330713404664237No Hit
TAATTGGGGCCACCTGGGATGGCCCTGGCCTGAATCCAGCCTCCGCTGTGGC790.11330713404664237No Hit
CTGATCGGGAGGTGCTTCATATACATTTCCTTTAACTCTGTGACATTCCTCC790.11330713404664237No Hit
GTTTTAGGGGAGTGATGAAATAATCTGTACAACACACCTCCAGGACACAAGT790.11330713404664237No Hit
CATTTAGGGAGAATTTTGGATATTTACATTTTTATTTTTTAACTCTTGGATC790.11330713404664237No Hit
CATCAAGGGAAGCAGTAGATAGTTCATCAATGAGAGAAGACCTTTTCAAACA790.11330713404664237No Hit
GACGTGGGATGACTTTAGATTGTTCTGTTTTCTTCCGTAGTGTATAATCAAT790.11330713404664237No Hit
TTTCGAGGGCGTGCCCTTGGGGCTCCGTGTCCTGCTGTCTTTCCGTCCGCTG780.11187286652706463No Hit
ATCGTTGGGGGACTAGAAAGCAGGTTGTTTTGTTTTGTTTTGTTTCCAAAGC780.11187286652706463No Hit
TTCATGGGAGAATTTCATATCTAGCCAAACTAAGCTTCATAAGCAAAGGAGA780.11187286652706463No Hit
TAAGCAGGGGGGCGCGCTTGCGCGCCAGCGGCTGCGGACACCAGCCTGCGTC780.11187286652706463No Hit
AACCTTGGGAGACACACAGGTTAATTATACATATTTTTAAATTTTGCAAAGG780.11187286652706463No Hit
GACAGGGGTGCTAACACTGAGGTAGCTCTCTTCATCTTATTTGCAAGCGGTC770.11043859900748687No Hit
CTGCTGGGGCATTGTGTACAGAAAGCATTTGTAGGGTGAGAAATGAAGGGCA770.11043859900748687No Hit
ATTACAGGGTATCTGTTTTTCCTGTTGTCAAATAAAATAAAATTCCAAACCA770.11043859900748687No Hit
TAACAGGGGATAATTGGCCATTGGTGGTCAACTCAGCCTGCAGCCCCTCTTT770.11043859900748687No Hit
GCATCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG770.11043859900748687No Hit
TTGCTAGGGCATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCA770.11043859900748687No Hit
CTCACCGGGGACGCTCTTCCGATCTTGGGCTGGGGGAGGGATAGCATTAGGA760.10900433148790913No Hit
TGTTAAGGGATTGTGATTCTGAAGCTTTCTCTTTAGGTCAGATATTGTTATA760.10900433148790913No Hit
GTTCCAGGGATGTCCACCGAAGTGGTCGAACTGCTCGAGCTACCAATGAAGG750.10757006396833137No Hit
GTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG740.10613579644875362No Hit
GAGCATGGGGAAGATAGATCATAACTTATCCAATCTGAATAACAGAGATTGT740.10613579644875362No Hit
GTAGGAACGGGCTCTAAAAAAATGCACTAAACCTCTAACTAATAAAATATTT740.10613579644875362No Hit
TCAAATGGGAACAGCAAATAACCATTGGTTAATCTGGACTTATTTTTGGACT740.10613579644875362No Hit
CTGGCTGGGAAGAGGTATAGTTATAAAACAAGACTACAGAAAAAGTTGTGGC740.10613579644875362No Hit
GTTGCAGGGACATGGATGAGGCTAGAAACCATCATTCTTAGCAAACTAACAA740.10613579644875362No Hit
AGAACAGGGAGTTTCTTAAAACGTATTTTCTGATGTGGCTTTACATTTCAAA730.10470152892917586No Hit
AAAGAAGGGCATTCAAGCTGAATTTTGCTCCAGTCTCCTTCAGTTTCCATCT730.10470152892917586No Hit
TTCCATGGGGAAGGTTAACATTATGTTTATTAAGTATCAAAATGGAATTATC730.10470152892917586No Hit
AGTATGGGGAATGGATAGAATTTTTTCATTCTATATATGATTACGGTTCACT730.10470152892917586No Hit
ATCGCAGGGAGATTAAGTCACAAAAATACAACTGTTTAGATAAATATTCAAG730.10470152892917586No Hit
TGACAAGGGATGAGAATTCAGCGAACTTGAAAGAAAAATCATCTGTGAGTTC730.10470152892917586No Hit
CATGAGGGGCAGAGAATTCTCAAGAGCAAGGGCTGCTCCCAGAATTCTTCCA730.10470152892917586No Hit
CGTTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA720.10326726140959812No Hit
CGATGGGGGCTGCACATCCCAACCCTTCACATTTTATAAATTATTGTAGATC720.10326726140959812No Hit
AACAATGGGTAGACATCCAAAACTAATCAATGCTGAGGTGGCTAAATACCTA720.10326726140959812No Hit
CATCCAGGGGCTTTGGCAGGGAAATATTACCAATGATAAGTAACGATAGATA720.10326726140959812No Hit
CCATTTCTCAAGATTCTGCGAAGGTGCTTTTCCTACCTCTTCTGACACCCTG720.10326726140959812No Hit
TTTAGGGGGCAGTTTCTTCGTTGGCACATAAATGTAGTAATACCTCCTCAGG720.10326726140959812No Hit
ACGCTTGGGAGGAGGGTACTTGTGTGATGTGGTTTATTTATTTTTTTGTTTC720.10326726140959812No Hit
ACTGGTGGGGATATTTTTTCAGAACAAGACCACTATTATTAACTAACTTGAA720.10326726140959812No Hit
CGTAAAGGGATGTTGAAACAAGCTTTGAATATTAATTTCGTTGAAGGTTTAT720.10326726140959812No Hit
AGCCATGGGACCATCTAGGGTTGTGTAAGTGCACACTGTGGAGCGCACAAAA720.10326726140959812No Hit
TATTAAGGGAAAGAAAAGAATTTTCAACCCGGCGTTTCATATCCAGCCAAAC720.10326726140959812No Hit
ATCATCGGGCGCTGCCTTTTTAAAATAGTGTGGTATTTCAAATATTGCTAGA720.10326726140959812No Hit
TCTCTGGGACAGGAAGTAGGGATATTTTTATAAAACAATTGTGTCCTCCCCA720.10326726140959812No Hit
ATTAAGGGAAGGAGAAGCTTTTTCTTCTTCCTATTTGTTTATTCATTTTGTT720.10326726140959812No Hit
GAACATGGGAATGTAAAGGGTTATAGCAGGTTTATAAGAATCTTATGGTTAA720.10326726140959812No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCCTGTCTCTTATAC710.10183299389002036No Hit
CATTAAGGGAATGCATGCTGGATTGCAGTATTTTTTTTAGAAGTTTATAAAT710.10183299389002036No Hit
GTTTATGGGGACAGTATTAAAGAAATGTATGGAATACAAAGATGAGTTATTC710.10183299389002036No Hit
CTTGCAGGGCTCCGATGCTCATTCTAATTCAAGGATGAGTCACCTTAATTTT710.10183299389002036No Hit
ACTTTAGGGAGAGTCTCACTGCTGGGGAGGAGAGGACTGGGCCTGATGGAAG710.10183299389002036No Hit
CACCTAGGGATCAGGATCCGGGACAGCCCTTGTACATTCTCTGGAATGCACA710.10183299389002036No Hit
TATAAAGGGAAGGCCTTTTCTTTCACAAAAAGCTGGGCCTACTGAACAATTC710.10183299389002036No Hit
AGATTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA710.10183299389002036No Hit
CTGCTTGGGACTAATGCAAAGATTACAGGGCCTTATTCCAAAGCAGTAATCC710.10183299389002036No Hit
AGCTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC710.10183299389002036No Hit
TTGTACGGGCAAGCCAGTTTCCAGAAGGGCTGGGGTGAGATCCTGACCTGTG710.10183299389002036No Hit
ACGCCTGGGAAATGGCACTGGCTCTACTACTAATTCTTCACTAACTTATTCC710.10183299389002036No Hit
AATTCAGGGTGAAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAAT710.10183299389002036No Hit
GGTTTTGGGAGAGGATAAACCGTTAGCAAATGAATGTAATAATTACTCATTT710.10183299389002036No Hit
AGATGAGGGAAGTTGTAGAGATGGATTGTGAGCATGTGACTGGTTTTCTTGT710.10183299389002036No Hit
CTACGAGGGATATATTCTAATCAGAGCCTTTAAAATATTTAATTTTTGTTTT700.10039872637044261No Hit
ACCGGGGATTCATTAAAGGTAAAGCCAATGGCCACCATTTCGTGTCATCTTC700.10039872637044261No Hit
ATAATGGGGAGAAATGTCTGGGTGTTTTATTTGCAGAGAACAAGATGTGTCT700.10039872637044261No Hit
TGACTTGGGGCCATACGTGAAAACTTAATTCTGCATGGAATCAGCGTCAGCG700.10039872637044261No Hit
TGCTTTGGGATGTTTGTGCATGTGTGACTGTGCATGTTATGTTTCTCCATGT700.10039872637044261No Hit
AACCGGGATGGCATACTAAGAATTTAACTTGTATAAATTACCTCTGAATAGT700.10039872637044261No Hit
TACTATGGGCAAGAAGCTCGGTGCACAGGTTTTCTCTGGTTCTTACAGGAAC700.10039872637044261No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)