FastQCFastQC Report
Sat 31 Dec 2022
SRR5846288.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846288.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68863
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT6270.9105034633983415RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC5290.7681919172850442No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1360.1974935741980454No Hit
AAATCGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1150.1669982428880531No Hit
AAGCCAAGGGTGGAGTGACATGCCCCAGATTATAAAATAGGTGGGCAGTGTG1110.16118960835281645No Hit
GCGTTTGGGCTGGCTTGCCTTCTCATTTTCTTAATAGTGTCCCTTGGAATAT1110.16118960835281645No Hit
GAACTGGGGGATCTAAAGCAGGGATTGGATGGATATGGGAGCTGTGGCCCTC1090.15828529108519815No Hit
AGAGGTGGGTTGGATGTTTCCTACAAAACTAAACACTTTTACCATAAGATCC1090.15828529108519815No Hit
CTCGCCGGGAGATGAGACATGTTGTAACCCTGTGGCCTGTTTTCTTATTTTG1070.15538097381757981No Hit
GGCACTGGGTAGACATCTTCATGCCCGTTAGTTCATCGTTTGCCTAGCATGT1060.15392881518377066No Hit
TCAAAAGGGGTTTGAAAATTATGTTCAGGGACTAGTAGGAGAGGGGAGCTAT1050.1524766565499615No Hit
ACGCAGGGGTTTATAAGAATTCTAACCATTGATATTCTTACATTCTTGCCTA1030.1495723392823432No Hit
GCGCGAAGGGAGTCAAAAGAACAGGAATTCGGAAAACTCTTCAGTAAATATC1030.1495723392823432No Hit
TTGTGCGGGAAGGGGACTGCTTTGAAGGGCCATTGTTCATTTGGAATGATAA1010.1466680220147249No Hit
AGCTCTGGGGATTTCCTGGTTTCCTCCTGCATTCTGCTTTACATTCTTGAAG1000.14521586338091574No Hit
GTTGTTGGGGTCACACTGGCCTTGCCCTTTCCTATCCTTAAGCTATGCCACC1000.14521586338091574No Hit
GGCTCTGGGCGCTGCCATAGTTTTTTACCACATTCCTAATGGTTTTCAACCT970.14085938747948826No Hit
GGTGCCGGGTCCTTATCAAAGAAGGGGCAAGCTCTTTTGCCTTTTAGGCCAG960.1394072288456791No Hit
CAAATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.1394072288456791No Hit
CTAACTGGGAGGCACTCACCAAATTCTTATTGAATGAATGAGTGAGTCAATG950.13795507021186992No Hit
TTCATAGGGGGACGCTGGGCCGGTGGGCGGGGGCCGGCAGGTGCTCCGCAGC930.13505075294425162No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA930.13505075294425162No Hit
CTGGCTGGGAAGAGGTATAGTTATAAAACAAGACTACAGAAAAAGTTGTGGC930.13505075294425162No Hit
TTAATGGGGATTCTGTGACGGAAAGCCTATGTTGACCTCGTCCGGCACTCAA920.13359859431044246No Hit
AGGCATGGGACATGTGTTTTTCTGGGGAAAATGGGTCCATGATACTCTAAGG920.13359859431044246No Hit
ATCATCGGGCGCTGCCTTTTTAAAATAGTGTGGTATTTCAAATATTGCTAGA910.1321464356766333No Hit
AAGATAGGGATGGGTTCAGTGGAAATGTTTGGAACTCTGAAGGATTTAGACA900.13069427704282416No Hit
GCAATTGGGGATCTTAATTGGGGTGATCTTTGTAGATTGGTTATTTCTGTAC900.13069427704282416No Hit
ACGCGGGAAAATATTAAACCTGGAAAATGTAGAAAGAAGAAATTATGAAGTA900.13069427704282416No Hit
TGCACTGGGAAGCAAGAGGAAAAAAACAACACAATCAAGGGACAAACTACAT890.129242118409015No Hit
TTTCGAGGGCGTGCCCTTGGGGCTCCGTGTCCTGCTGTCTTTCCGTCCGCTG890.129242118409015No Hit
TTCTTGGGCTGATGCTGTGGTCCTTTGTGATAGTAGAGCACCGGGGTTAACC890.129242118409015No Hit
ATGCAGGGGACCCATTTGAGTGATATTGCACAACAAACACAGATACCTACAG880.12778995977520585No Hit
CGTTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.12778995977520585No Hit
TTAAAGGGGGCCTCAAGCCCTGCCTCTTGTCACAGTGAGGGTTCTGAGAATA880.12778995977520585No Hit
GAGCATGGGGAAGATAGATCATAACTTATCCAATCTGAATAACAGAGATTGT870.1263378011413967No Hit
CGTTTGGGGATTTTAACAAAGTAATATTTTTGTATTGCATTTTTCTATTAAA870.1263378011413967No Hit
ACTTTTGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG870.1263378011413967No Hit
TTTAGGGGGCAGTTTCTTCGTTGGCACATAAATGTAGTAATACCTCCTCAGG870.1263378011413967No Hit
GCATCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG870.1263378011413967No Hit
CAAAAAGGGAGGTGGTTCTCCGTGTGTTTATATACACAGACGATCCCCGAAT870.1263378011413967No Hit
TTTTAAGGGCAGCTGGCATGTAATGAGATTTTTATTTGCATGGCTTTTTTTT870.1263378011413967No Hit
AATGAGGGGACGTTACAGATTCGAAGAGAGAGAGGACGACCGTTCTTCCCTC870.1263378011413967No Hit
GATTGGGATAGTGCCAACACGTGGCGCGCCTTTGATGGCCCGGGTGCACACC860.12488564250758753No Hit
TAACCAGGGCATCAAGTGCATAGCTTTTAGCTTTTAACTGAATTTTTCTGGG860.12488564250758753No Hit
CTGATCGGGAGGTGCTTCATATACATTTCCTTTAACTCTGTGACATTCCTCC850.12343348387377838No Hit
GTTTATGGGGACAGTATTAAAGAAATGTATGGAATACAAAGATGAGTTATTC850.12343348387377838No Hit
TAAGCTGGGTGTTGAGTGAATTAATGAATTAAGAGTGGCTGGCAGAGTAGGC850.12343348387377838No Hit
TTCATGGGAGAATTTCATATCTAGCCAAACTAAGCTTCATAAGCAAAGGAGA850.12343348387377838No Hit
ACTTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC850.12343348387377838No Hit
TTCCGGGGTCATTTCTAATTGTGTTTATTTGAATCTTCTCTCTTTTCTTTTT840.12198132523996923No Hit
TACAGAGGGGGACTCTCTCTGTAAGGAGGGAAGGGGGAGAAGGTAGAATTCT840.12198132523996923No Hit
CACTAGGGGAATGCTGCAAATTATCAGTATTTATAAAGTAACTGATTTTGCA840.12198132523996923No Hit
GCTGCGGGGAAAGGCAACAGGCTGTTATGCAGAGCCTGTTGCCTTTCCTCGC840.12198132523996923No Hit
AAAGAAGGGCATTCAAGCTGAATTTTGCTCCAGTCTCCTTCAGTTTCCATCT830.12052916660616006No Hit
GTTACTGGGACCGGGAAACCACTGATCAATCAAGGAATTGACGTGGTCCGTG830.12052916660616006No Hit
TACTAGGGGAGCTTTTGAAGAAAGGCCAGTGATCCAGCAAAGGGGGAAAAAT830.12052916660616006No Hit
CATTGTGGGAATCACTTTCTAAAGAATCTTTATAAGAGATAGTTTACACCAC830.12052916660616006No Hit
GTTTTAGGGCTTCTTTCCCATCTGGCGGCCGCGGCTCCTGTCCAGACCCTGA830.12052916660616006No Hit
TTGCTGGGGCGATCTTACTTGGGGGTTGTTTTTTTGAAAAGATCAACAAAAT830.12052916660616006No Hit
GACAGGGGTGCTAACACTGAGGTAGCTCTCTTCATCTTATTTGCAAGCGGTC820.11907700797235089No Hit
CTACGAGGGATATATTCTAATCAGAGCCTTTAAAATATTTAATTTTTGTTTT820.11907700797235089No Hit
ATTTATGGGTGTTCTGGCAATTCTTGTTTCTTGATTTTTCTATATAAATTTT820.11907700797235089No Hit
GACAGTGGGGTTAATTCATGAACAGGATATAACAAATGTAAATATATATGCA820.11907700797235089No Hit
ATAAATGGGTACAAAAGAAAACCTGTAGAAGTTTTTACAAATATAAGAAATT810.11762484933854174No Hit
ACCTTGGGACAACATGTTGGTAGATAAACTTAATACCAACACTGAAATGGAA810.11762484933854174No Hit
GAGAAAGGGCTGGTGATTCAAGTGAATTTTAAAGCATGTTTATGCTGTTGCA810.11762484933854174No Hit
CTTCATGGGAAGACATTATTTTGCTCATAAAAGTCATTGTGTATCTTACCAA810.11762484933854174No Hit
ACATTAGGGAACCTCAACAAAGCACCTAAAAACAGTATTTTCTTATGTAAGA800.11617269070473257No Hit
TGTCTGGGGTTAGAAACTTACTGGAAATGCTGACACGGATTTTATGCCTTTT800.11617269070473257No Hit
CTGCCGGGGAAAGTGAATGGATGACTGTTCATGAGAAAAAGCAGGGTAAAAA790.11472053207092342No Hit
CTGCTGGGGCATTGTGTACAGAAAGCATTTGTAGGGTGAGAAATGAAGGGCA790.11472053207092342No Hit
AGACTTGGGAGTAGAAACTGTGAACTCTGTGACTTTGACTCAGGACACAGAA790.11472053207092342No Hit
CGAATGGGGAACCCTCTGTAGCTCACACCGTTTTCTGAACTATAAAATGGAG790.11472053207092342No Hit
AGACTAGGGATAACGTGAATATTTATACAGTAGCCTCTTCAGGCTTTGTAAT790.11472053207092342No Hit
TGTTAAGGGATTGTGATTCTGAAGCTTTCTCTTTAGGTCAGATATTGTTATA790.11472053207092342No Hit
TCAAATGGGAACAGCAAATAACCATTGGTTAATCTGGACTTATTTTTGGACT790.11472053207092342No Hit
TACTATGGGCAAGAAGCTCGGTGCACAGGTTTTCTCTGGTTCTTACAGGAAC790.11472053207092342No Hit
AGATTAGGGCACGGTAAACTTCATTCATAGATCCTCCCTTATTTAACATCAG780.11326837343711427No Hit
GCATGGGCACAAATATATGGTATACATAGCATATAGAAAGAAAACTAGACCA780.11326837343711427No Hit
GTAGGAACGGGCTCTAAAAAAATGCACTAAACCTCTAACTAATAAAATATTT780.11326837343711427No Hit
TTCGCTGGGGACCAGCATACAAGTATAGAGACCTAGGAGGTGGTCTTGTGGT780.11326837343711427No Hit
CTCGCTTCGGGAAGTGCTGGGATTACAGGTGTGATCCACTGCACCCGGCCGG780.11326837343711427No Hit
TGCCGGGGCTCTGCCTTTGGTGTTTGTACATGTTAAGAATTGACCAGTGAAG770.11181621480330511No Hit
GGCGATGGGGAAACACAAAGAGCATTCTAAAAGAAACACAATTATCTATAAA770.11181621480330511No Hit
AGAACAGGGAGTTTCTTAAAACGTATTTTCTGATGTGGCTTTACATTTCAAA770.11181621480330511No Hit
TAGATGGGGGGAATTACAATTATGCCAAACAACAGTGAAAGCACATCTAGTA770.11181621480330511No Hit
AGCCATGGGCTGTGTCTGGGCAATGACAGTCGCATAAGGATTAGGCTCACTC770.11181621480330511No Hit
CGGCCGGAGGGTGTGTCTTTGTACTTGGTTCATTGCCCTTCACCTGGTAGAG770.11181621480330511No Hit
TAAATGGGGTGTCCCTGACAAAACCTACAAAAAGTGACTTTAACACTTCAGG760.11036405616949596No Hit
GTCAACGGGGTAACCTTGGTGTGATGTAATAATGACATTATCTGCTGGTCTT760.11036405616949596No Hit
GTTTCAGGGTGGTGCAGATATGAGAAGGTGATTACAGGTAGACCAATGGGAG760.11036405616949596No Hit
CATCAAGGGAAGCAGTAGATAGTTCATCAATGAGAGAAGACCTTTTCAAACA760.11036405616949596No Hit
CTGCTTGGGACTAATGCAAAGATTACAGGGCCTTATTCCAAAGCAGTAATCC760.11036405616949596No Hit
GGTAGTGGGCCAATGGGAAAGGCAACAAGTAACATAAAGGCATTTGTCTTCA760.11036405616949596No Hit
CTTTTGGGGGCTCTGAGAGATTCCTCTGCGAATCTGTTTTTAGCTATCAAGT750.1089118975356868No Hit
GTATCAGGGAGTGCTGAAGACAAGTTTTCTAAAGTGTATTATGGTGATAAAT750.1089118975356868No Hit
CTCACCGGGGACGCTCTTCCGATCTTGGGCTGGGGGAGGGATAGCATTAGGA750.1089118975356868No Hit
TATTAAGGGAAAGAAAAGAATTTTCAACCCGGCGTTTCATATCCAGCCAAAC750.1089118975356868No Hit
GAACATGGGAATGTAAAGGGTTATAGCAGGTTTATAAGAATCTTATGGTTAA750.1089118975356868No Hit
GAAGTCGGGGGGCAGACCAGGGCCAGGTAGACGGGGCTGTTTGGTGCCTGAA740.10745973890187764No Hit
CTTCATCGGGCGATCTGTATTAGGGATGACTTTCCGTTGTGTACACTCACAC740.10745973890187764No Hit
GTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG740.10745973890187764No Hit
TCTAATGGGGAACACACATTGGAGAGAAACACTATGAATGTAAGGAATGCGG740.10745973890187764No Hit
AAATCAGGCTTTCTCAGTGTAACTGCATTTCTAATAAAAATTTATTGAGTGA740.10745973890187764No Hit
TATAAAGGGAAGGCCTTTTCTTTCACAAAAAGCTGGGCCTACTGAACAATTC740.10745973890187764No Hit
TAATCCGGGAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACA740.10745973890187764No Hit
TAAGCAGGGGGGCGCGCTTGCGCGCCAGCGGCTGCGGACACCAGCCTGCGTC740.10745973890187764No Hit
ACCGGGGATTCATTAAAGGTAAAGCCAATGGCCACCATTTCGTGTCATCTTC740.10745973890187764No Hit
ACTTTCGGGTAAGATGAAAAGGGTTGAGATGATGAGATGAAATGAGATGAAA740.10745973890187764No Hit
ATCGCAGGGAGATTAAGTCACAAAAATACAACTGTTTAGATAAATATTCAAG740.10745973890187764No Hit
TTGAATGGGACATTTTAGTTTAGTTTTTAGATTTTTTTTTAAACGATATATT740.10745973890187764No Hit
AATGCTGGGGAGTCAGTCTGACGGTCAGTGGATCGGTGGGTTTATCTCAAGG730.10600758026806849No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAAC730.10600758026806849No Hit
AGTAACGGGCTTCCGATCTCAGTCTGGGACAACATTAATCCATTAAAGCTAA730.10600758026806849No Hit
TCACTAGGGATGTCAGTTTGCAGTTATGCAGAATCCAAAGTAAATGTCCTGC730.10600758026806849No Hit
AGCTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC730.10600758026806849No Hit
AGCCATGGGACCATCTAGGGTTGTGTAAGTGCACACTGTGGAGCGCACAAAA730.10600758026806849No Hit
AACTCAGGGGTATTCAATTTCTCTACTGAAAAATTAATATAAAAGCAATCAC720.10455542163425934No Hit
GTTTTAGGGCCTGTTCTTACTCTTCATTTAAAGAGATTTCAGCAGGCTGGTT720.10455542163425934No Hit
ATTATTGGGGAGGCTCGCTGCGCTCCCTGTTGCCCAGCGCGGGCCCGTTGAG720.10455542163425934No Hit
TGCTTTGGGATGTTTGTGCATGTGTGACTGTGCATGTTATGTTTCTCCATGT720.10455542163425934No Hit
GTGTCGGGGCTCGGACAACAGCAGCCTCATGTTTCCCATCCTGGTGGCCTTC720.10455542163425934No Hit
AAATAGAGGGTATCATCAGCAAACAGTGACAGTTTGACTTCCTCTATACCGA720.10455542163425934No Hit
GACGTGGGATGACTTTAGATTGTTCTGTTTTCTTCCGTAGTGTATAATCAAT720.10455542163425934No Hit
AGACCTGGGATCTCAAAATGAGATTTGGGCAGGGACAAATATCCAAACTATA710.10310326300045018No Hit
CATTAAGGGAATGCATGCTGGATTGCAGTATTTTTTTTAGAAGTTTATAAAT710.10310326300045018No Hit
GGGTTCCGGGTTATTTGAAAATCCAGTCCTTTATTAATAGAATGGAGGAAAG710.10310326300045018No Hit
CGCTTAGGGGAGTGATAGAACTCCTAGAAAATTTGCAAAGAAATGTTACATA710.10310326300045018No Hit
GAGCTGGGGACGGAGGCCACAGGATCCCAGCCCGGCCTTTGTGGGACTGAGG710.10310326300045018No Hit
GTTTCAGGGGCTCACTGTAGCCTCAATTTCCTAGGCTCAAGGGATCCTCCTG710.10310326300045018No Hit
AATACTGGGAGCTATTGTTTCTTCAGTCCAAATGGTCATACTTTGGGTGATG710.10310326300045018No Hit
TTTCTAGGGTTTCTGGAACATTTATCAAGGACAATTTGCTAGAAGAAATGGA710.10310326300045018No Hit
GTCATGGGGTGGTTAGTGAGAAGTAAAATAATGGAAAGTAAAATAATAAAAG700.10165110436664102No Hit
TAACAGGGGATAATTGGCCATTGGTGGTCAACTCAGCCTGCAGCCCCTCTTT700.10165110436664102No Hit
ACGCTTGGGAGGAGGGTACTTGTGTGATGTGGTTTATTTATTTTTTTGTTTC700.10165110436664102No Hit
AAGTAAGGGAGGGCTCTGCGGTTTCTGGCATCTCCAAGCTTCCAGGTGCCAC700.10165110436664102No Hit
TAAGTGGGGCCGTTAATTTGTTGAATTAAATGCCTTTATAAAAATATTTACA700.10165110436664102No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG700.10165110436664102No Hit
CTGCAGGGGGGCCCTTTTCTCCAGCAACTGCAGAGGCATTTCAGGAGTTGGA700.10165110436664102No Hit
AATTCAGGGTGAAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAAT700.10165110436664102No Hit
TGCATGGGGACAAGGGTCTTCAGTCTAATGTCCCTCATCTCTGTAACTCCAT690.10019894573283185No Hit
CACTTAGGGAAATATGTGAAATCTCTGACCAATACTGTGACAGCTCTATTAT690.10019894573283185No Hit
CTTGCAGGGCTCCGATGCTCATTCTAATTCAAGGATGAGTCACCTTAATTTT690.10019894573283185No Hit
CATCCAGGGGCTTTGGCAGGGAAATATTACCAATGATAAGTAACGATAGATA690.10019894573283185No Hit
TCCGCGGGCCCTACCTTGCACCCTCTGAGTTCTCTTTGGACTTGTGGAAGAG690.10019894573283185No Hit
AATTAAGGGCATTACTGTAACCTTATAGTGTCATTTGAAGTAGGTAATGTGA690.10019894573283185No Hit
GTTAGAGGGGGACACTAGGGAATAGATGTACATTTTGTATTGGGGGTTCCAA690.10019894573283185No Hit
TGCTGCGGATGGCTCTAACTTCTGTGTTGCTGTTTATCTTGTTATTTTTCGG690.10019894573283185No Hit
GCGTAGCGGGGAATGTCAAGGTTCTTAAGAGTTTAAGAAAGATGAAAAATTT690.10019894573283185No Hit
ATAATGGGGAGAAATGTCTGGGTGTTTTATTTGCAGAGAACAAGATGTGTCT690.10019894573283185No Hit
GACAAAGGGAGCTCTAATAATTCTGTAGCAAAGCCAATACAAAAATCAGCTA690.10019894573283185No Hit
CGTAAAGGGATGTTGAAACAAGCTTTGAATATTAATTTCGTTGAAGGTTTAT690.10019894573283185No Hit
ACGCTAGGGGGCTCAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTC690.10019894573283185No Hit
ACGTACGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC690.10019894573283185No Hit
AGCTGGGGGGTTCTCTACACTTACTGTAATACTGATTATTCTTACATATCAA690.10019894573283185No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)